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pDONR223_ZBTB24_p.G568C

Plasmid

#82783

Purpose

Gateway Donor vector containing ZBTB24, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ZBTB24 (ZBTB24 Human)

Use

Gateway entry vector

Mutation

G568C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3R3_p.M295T

Plasmid

#82776

Purpose

Gateway Donor vector containing PIK3R3, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PIK3R3 (PIK3R3 Human)

Use

Gateway entry vector

Mutation

M295T; D338A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3R3_p.R245C

Plasmid

#82777

Purpose

Gateway Donor vector containing PIK3R3, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PIK3R3 (PIK3R3 Human)

Use

Gateway entry vector

Mutation

R245C; D338A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ZNF597_p.L283V

Plasmid

#82755

Purpose

Gateway Donor vector containing ZNF597, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ZNF597 (ZNF597 Human)

Use

Gateway entry vector

Mutation

L283V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3R3_p.F380L

Plasmid

#82764

Purpose

Gateway Donor vector containing PIK3R3, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PIK3R3 (PIK3R3 Human)

Use

Gateway entry vector

Mutation

D338A; F380L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3R3_p.P127L

Plasmid

#82767

Purpose

Gateway Donor vector containing PIK3R3, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PIK3R3 (PIK3R3 Human)

Use

Gateway entry vector

Mutation

P127L; D338A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ZNF597_p.V21L

Plasmid

#82753

Purpose

Gateway Donor vector containing ZNF597, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ZNF597 (ZNF597 Human)

Use

Gateway entry vector

Mutation

V21L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RIT1_p.R122L

Plasmid

#82737

Purpose

Gateway Donor vector containing RIT1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RIT1 (RIT1 Human)

Use

Gateway entry vector

Mutation

R122L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STC2_p.R5W

Plasmid

#82744

Purpose

Gateway Donor vector containing STC2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STC2 (STC2 Human)

Use

Gateway entry vector

Mutation

R5W

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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HRas (G12V, E37G)-pcw107

Plasmid

#64639

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

HRAS (transcript variant 3) (HRAS Human)

Use

Lentiviral

Mutation

G12V, E37G

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
HRas (G12V, E37G)-pcw107-V5

Plasmid

#64640

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

HRAS (transcript variant 3) (HRAS Human)

Use

Lentiviral

Tags

V5

Mutation

G12V, E37G

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-dCas9-p300 Core (C1204R)

Plasmid

#61361

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter

Depositor

Charles Gersbach

Article

Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

Insert

S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)

Use

CRISPR and Synthetic Biology

Tags

Flag, HA, and SV40 NLS

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9; C1204R mutation i…

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-B

Plasmid

#104448

Purpose

Expression of human VAP-B fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein B (VAPB Human)

Tags

EGFP and HA

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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CRISPRoff-v2.1

Plasmid

#167981

Purpose

Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter

Depositor

Luke Gilbert

Article

Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

Insert

CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)

Tags

2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP

Expression

Mammalian

Promoter

CAG

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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TETv4

Plasmid

#167983

Purpose

Expresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoter

Depositor

Luke Gilbert

Article

Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

Insert

TETv4 (TET1CD-XTEN80-dCas9)

Use

CRISPR and Synthetic Biology

Tags

3xNLS, TET1 catalytic domain, and tagBFP

Expression

Mammalian

Promoter

CAG

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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anxA2-GFP

Plasmid

#107196

Purpose

Expresses human annexin A2-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

annexin A2 (ANXA2 Human)

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

Ala residue 65 in human sequence replaced by Glu …

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-A

Plasmid

#104447

Purpose

Expression of human VAP-A fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_HyPer7_mito

Plasmid

#238968

Purpose

Mammalian neurons HyPer7 expression targeted to the mitochondrial matrix

Depositor

Dmitry Bilan

Article

Kelmanson et al Redox Biol. 2021 Nov 3;48:102178. doi: 1

Insert

HyPer7

Use

AAV

Tags

3x Cytochrome C Oxidase Subunit VIII presequense

Expression

Mammalian

Promoter

hSyn1

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO SF-HERC2 (ShB-R)

Plasmid

#55613

Purpose

Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cells

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (WT) (HERC2 Human)

Tags

3xFLAG and STREP

Expression

Mammalian

Mutation

Silent mutations have been incorporated into the …

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro

Plasmid

#83306

Purpose

Lentivirus vector to express guideRNA and dCas9 with puro resistant gene

Depositor

Tohru Kimura

Article

Murakami et al Nat Immunol. 2021 Mar;22(3):301-311. doi

Insert

sgRNA and dCas9 from pX330

Use

Lentiviral

Tags

FLAG and PA tags

Expression

Mammalian

Promoter

U6 for sgRNA and CBh for dCas9

Available Since

Dec. 13, 2016

Availability

Academic Institutions and Nonprofits only

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