We narrowed to 9,182 results for: Pol

1. 4MYJ

   Plasmid

   #60104

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PF14_0346:MAC04G-A11:C228843
   

   Use

   Baculovirus expression

   Tags

   6xHis-TEV and MHHHHHHSSGRENLYFQG

   Mutation

   See comments

   Promoter

   Polyhedrin

   Available Since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

2. p404GALL

   Plasmid

   #17418

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   GALL promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   GALL promoter is between SacI and XbaI restrictio…

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. PLXNB1

   Plasmid

   #25352

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PLXNB1:Y1511-L2135 (PLXNB1 Human)
   

   Use

   Baculovirus expression

   Tags

   His

   Promoter

   polyhedrin

   Available Since

   Aug. 19, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

4. PB-Puro-U6-gRNA-pA plasmid

   Plasmid

   #247671

   Purpose

   spCas9 gRNA is driven by human U6 promoter, with the gRNA cassette between PuroR and pA, making it detectable in polyA-based RNA-seq.

   Depositor

   Tae Hyun Kim

   Insert

   Puromycin resistant gene
   

   Expression

   Mammalian

   Promoter

   TK promoter

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. NSP12:4393-5324_Nbio_Chis_BV

   Plasmid

   #234377

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   Nsp12_SARS2
   

   Use

   Baculovirus expresssion

   Tags

   GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

   Promoter

   Polyhedrin

   Available Since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. NSP13:5325-5925_BVnAvi

   Plasmid

   #234375

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   Nsp13_SARS2
   

   Use

   Baculovirus expresssion

   Tags

   GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

   Promoter

   Polyhedrin

   Available Since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. SARS1_NSP13_BV_NBIO-TEV_CTHROM-his

   Plasmid

   #234382

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   Nsp13_SARS
   

   Use

   Baculovirus expresssion

   Tags

   LVPRGSGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

   Promoter

   Polyhedrin

   Available Since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pD(P+T)_CMV

   Plasmid

   #223860

   Purpose

   4G-cloning donor for mammalian promoter and terminator, no tag added

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   CMV promoter and bGH polyA
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac HTB His-TEV-BN-rNLRP1-FLAG

   Plasmid

   #217323

   Purpose

   Purification of Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cells

   Depositor

   Yifan Wang

   Insert

   NLRP1
   

   Tags

   FLAG and His-TEV

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac HTB His-TEV-LEW-rNLRP1-FLAG

    Plasmid

    #217320

    Purpose

    Purification of Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells

    Depositor

    Yifan Wang

    Insert

    NLRP1
    

    Tags

    FLAG and His-TEV

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW57.1-GFP_mmu-miR-16-5p_sponge

    Plasmid

    #132624

    Purpose

    Expresses a TurboGFP transcript with microRNA sponge for mmu-miR-16-5p as 3'UTR

    Depositor

    Constance Ciaudo

    Insert

    stop codon, mmu-miR-16-5p sponge sequence and polyA signal
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. UTP15:PBC028-C01:C245785

    Plasmid

    #183973

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    UTP15 (UTP15 Human)
    

    Use

    Baculovirus expresssion

    Tags

    6xHis-TEV

    Promoter

    Polyhedrin

    Available Since

    May 10, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. LIPS:SDC101a-A08:C34411

    Plasmid

    #183978

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    LIPS
    

    Use

    Baculovirus expresssion

    Tags

    C-terminal 8xHis tag:EFVEHHHHHHHH and N-termina…

    Promoter

    Polyhedrin

    Available Since

    May 10, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCDZ2FE_Rev3_1873-2025

    Plasmid

    #105672

    Purpose

    Expresses Flag-tagged fragment (amino acids 1873-2025) of human Rev3 protein

    Depositor

    Thanos Halazonetis

    Insert

    Rev3L (REV3L Human)
    

    Tags

    2X Flag tag

    Expression

    Mammalian

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOpen-ECOligA

    Plasmid

    #165564

    Purpose

    Connects preferentially cohesive double-stranded DNA ends, active on blunt end DNA in the presence of Ficoll or polyethylene glycol. Requires Mg2+ and NAD+. Ligation when blunt end or RNA/ DNA ligation needs to be avoided.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    E. coli DNA Ligase
    

    Use

    Synthetic Biology

    Available Since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pTU1-D-spsCD

    Plasmid

    #112788

    Purpose

    Downstream homology arm, SpsC is involved in spore coat polysaccharide synthesis

    Depositor

    Chris French

    Article

    Wicke et al bioRxiv 185108

    Insert

    spsCD
    

    Expression

    Bacterial

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTU1-A-spsCU

    Plasmid

    #112787

    Purpose

    Upstream homology arm, SpsC is involved in spore coat polysaccharide synthesis

    Depositor

    Chris French

    Article

    Wicke et al bioRxiv 185108

    Insert

    spsCU
    

    Expression

    Bacterial

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. p404GALS

    Plasmid

    #17435

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    GALS promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    GALS promoter is between SacI and XbaI restrictio…

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac1_FLAG-BPTF

    Plasmid

    #102242

    Purpose

    Expression of human Flag-BPTF using baculovirus from pFastBac1

    Depositor

    Joe Landry

    Insert

    Flag-BPTF (BPTF Human)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    Polyhedron

    Available Since

    Dec. 11, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. 53 pLIB_MBP

    Plasmid

    #160301

    Purpose

    N-term 3C cleavable MBP tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    6xHis and MBP

    Expression

    Insect

    Promoter

    polyhydrin

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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