We narrowed to 17,014 results for: EMB

1. pMS32: pTwinStrep-SUMO-PmcTnsC

   Plasmid

   #168165

   Purpose

   PmcTnsC expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Insert

   PmcCAST TnsC
   

   Tags

   6X His, Twin Strep and SUMO

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. T7-RelA(K218R)

   Plasmid

   #23247

   Depositor

   Warner Greene

   Article

   Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

   Insert

   NFkB RelA(K218R) (RELA Human)
   

   Tags

   T7 gp10

   Expression

   Mammalian

   Mutation

   K218R

   Available Since

   March 15, 2010

   Availability

   Academic Institutions and Nonprofits only

3. MSCV Puro-SV40:GFP

   Plasmid

   #68483

   Purpose

   Retrovirus expressing GFP driven by SV40 promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Puromycin Resistance
   GFP
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR and SV40

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Phat2-hs-cPla2-C2-EGFP-GH

   Plasmid

   #187088

   Purpose

   Protein Expression

   Depositor

   Philipp Niethammer

   Article

   Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

   Insert

   ENSG00000116711 (PLA2G4A Human)
   

   Tags

   EGFP

   Expression

   Bacterial

   Available Since

   Aug. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag TSC2 2A(S939A + S981A)

   Plasmid

   #12131

   Depositor

   Cheryl Walker

   Article

   Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

   Insert

   TSC2 S939A S981A (TSC2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S939A. S981A.

   Available Since

   July 6, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pDGB3_alpha1

   Plasmid

   #68228

   Purpose

   GoldenBraid level 1 Alpha plant expression vector.

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   empty vector
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Col1A-5'probe

   Plasmid

   #20732

   Depositor

   Rudolf Jaenisch

   Article

   Beard et al Genesis. 2006 Jan . 44(1):23-8.

   Insert

   Col1A-5'probe (Col1a1 Mouse)
   

   Expression

   Bacterial

   Available Since

   April 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRU52

   Plasmid

   #167678

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   PcUB4-2::spCas9
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEG BacMam C term 3C eGFP myc

   Plasmid

   #160685

   Purpose

   Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

   Depositor

   Eric Gouaux

   Article

   Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

   Type

   Empty backbone

   Tags

   His8 eGFP 3C

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. gRNA-mCtnnb1

    Plasmid

    #84542

    Purpose

    mCtnnb1 gRNA expressing vector

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

    Insert

    mCtnnb1 gRNA
    

    Use

    CRISPR

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5 FRT/TO MycLAP-STIL 5A

    Plasmid

    #80267

    Purpose

    mammalian expression plasmid for MycLAP-STIL 5A

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    STIL (STIL Human)
    

    Tags

    Myc-GFP

    Expression

    Mammalian

    Mutation

    S1103A, S1108A, S1111A, S1116A, T1119A

    Promoter

    CMV

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3 Flag DLC2

    Plasmid

    #24269

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    DLC2 (Dynll2 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pDsRed (GB0100)

    Plasmid

    #68202

    Purpose

    Provides the CDS of DsRed as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    DsRed coding region
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. MIS12-FRB-tagRFP

    Plasmid

    #114039

    Purpose

    Kinetochore localised FRB tagged with FLAG and tagRFP

    Depositor

    Geert Kops

    Article

    Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

    Insert

    MIS12 (MIS12 Human)
    

    Tags

    FRB-FLAG-tagRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-EGFP-C1-HsLSm1_J

    Plasmid

    #146676

    Purpose

    Mammalian Expression of HsLSm1

    Depositor

    Elisa Izaurralde

    Article

    Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

    Insert

    HsLSm1 (LSM1 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEVOL-MmAcKRS1-PylTUUA

    Plasmid

    #182537

    Purpose

    N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion with ochre suppression

    Depositor

    Wenshe Liu

    Article

    Wu et al Chembiochem. 2012 Jul 9;13(10):1405-8. d

    Insert

    PylTUUA
    

    Expression

    Bacterial

    Promoter

    ProK

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGSTag Flag Jnk3a2

    Plasmid

    #15748

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk3a2 (MAPK10 Human)
    

    Expression

    Bacterial

    Available Since

    March 6, 2008

    Availability

    Academic Institutions and Nonprofits only

18. GL2 shRNA

    Plasmid

    #86083

    Purpose

    A lentiviral construct for building stable cell lines expressing GL2 shRNA via puromycin selection.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    control shRNA
    

    Promoter

    U6

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA 3.1 (+) Tacδ

    Plasmid

    #81177

    Purpose

    Mammalian expression of human Tacδ (GFFKR cytoplasmic tail)

    Depositor

    Chinten James Lim

    Article

    Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

    Insert

    Tacδ
    

    Tags

    KLGFFKR

    Expression

    Mammalian

    Mutation

    CD25 amino acids 1 to 263 fused to KLGFFKR cytopl…

    Promoter

    CMV

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMaCTag-Z09

    Plasmid

    #120052

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mVenus

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mVenus
    

    Use

    Pcr template

    Tags

    mVenus

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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