We narrowed to 10,134 results for: ada

1. hU6-pegRNA-EFS-mScarlet Template

   Plasmid

   #196037

   Purpose

   The template UPEmS vector designed for cloning of a single pegRNA and subsequent co-expression with mScarlet.

   Depositor

   Tyler Jacks

   Article

   Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

   Insert

   mScarlet
   

   Expression

   Mammalian

   Promoter

   hU6 and EFS

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. kiCAP-AAV-MyoAAV1C

   Plasmid

   #224438

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 1C, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Mutation

   RGDLSTP insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-PE2-P2A-BSD

   Plasmid

   #174819

   Purpose

   Mammalian expression of SpCas9 prime editor 2 with P2A-BSD marker

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PE2-P2A-BSD
   

   Tags

   P2A-BSD and SV40 bpNLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTwist-SARS-CoV-2 Spike-deltaC18 XBB.1.16

   Plasmid

   #212532

   Purpose

   Encodes SARS-CoV-2 variant XBB.1.16 Spike for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike XBB.1.16 (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG416-Gal-PR50

   Plasmid

   #84901

   Purpose

   induce expression of dipeptide repeat PR in yeast

   Depositor

   Aaron Gitler

   Article

   Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

   Insert

   PR50 (C9orf72 Synthetic)
   

   Tags

   FLAG and Myc

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLKO.1-puro-Nono-sh5

   Plasmid

   #127651

   Purpose

   Knock-down of human NONO

   Depositor

   Nicolas Manel

   Article

   Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

   Insert

   NONO shRNA (NONO Human)
   

   Use

   Lentiviral

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLKO.1-puro-Nono-sh1

   Plasmid

   #127650

   Purpose

   Knock-down of human NONO

   Depositor

   Nicolas Manel

   Article

   Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

   Insert

   NONO shRNA (NONO Human)
   

   Use

   Lentiviral

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCG_SARS-CoV2 ORF8 C-V5-tag

   Plasmid

   #179990

   Purpose

   Mammalian expression vector for SARS-CoV-2 ORF8, V5-tagged. Sequence codon-optimized.

   Depositor

   Konstantin Sparrer

   Article

   Hayn et al Cell Rep. 2021 May 18;35(7):109126.

   Insert

   SARS-CoV-2 ORF8 (ORF8 Synthetic)
   

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLVE-hTGFB3-IRES-Red

   Plasmid

   #52580

   Purpose

   2nd generation lentiviral transfer vector driving expression of human TGF-beta3 and RFP in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

   Insert

   human transforming growth factor-beta3 (TGFB3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1alpha

   Available Since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAG416-Gal-PA50

    Plasmid

    #84902

    Purpose

    induce expression of dipeptide repeat PA in yeast

    Depositor

    Aaron Gitler

    Article

    Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

    Insert

    PA50 (C9orf72 Synthetic)
    

    Tags

    FLAG and Myc

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-HNF1A-WT-Flag-UTR

    Plasmid

    #183237

    Purpose

    Expression of FLAG tagged HNF1A

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    HNF1 homeobox A (HNF1A Human)
    

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYX233-b2-DHFR

    Plasmid

    #163759

    Purpose

    Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)-DHFR

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Insert

    b2-DHFR (Dhfr Budding Yeast, Mouse)
    

    Expression

    Yeast

    Mutation

    Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

    Promoter

    GAL1

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSPL3-ABCC8-9-10

    Plasmid

    #135918

    Purpose

    Encodes ABCC8 exons 9 and 10 wild type for the analysis of splicing variants

    Depositor

    Diana Valverde Pérez

    Article

    Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

    Insert

    ATP-binding Cassette subfamily C member 8 (ABCC8 Human)
    

    Use

    Exon trapping

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAG416-Gal-GA50

    Plasmid

    #84903

    Purpose

    induce expression of dipeptide repeat GA in yeast

    Depositor

    Aaron Gitler

    Article

    Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

    Insert

    GA50 (C9orf72 Synthetic)
    

    Tags

    FLAG and Myc

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPB-cT3G-cERP2-MYC

    Plasmid

    #192899

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of MYC

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    MYC (MYC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNOC_dfnCas12a-KRAB_crRNA NC

    Plasmid

    #176262

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleas

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    Kruppel associated box domain and luciferase

    Mutation

    D917A and E1006A mutations to inactivate the endo…

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RRL.sin.cPPT.SFFV/Ace2.IRES-neo.WPRE (MT129)

    Plasmid

    #145840

    Purpose

    Lentiviral vector genome to ectopically express human Ace2

    Depositor

    Caroline Goujon

    Article

    Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

    Insert

    human Ace2 (ACE2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA

    Plasmid

    #134922

    Purpose

    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCG_SARS-CoV2 ORF7a C-V5-tag

    Plasmid

    #179981

    Purpose

    Mammalian expression vector for SARS-CoV-2 ORF7a, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    SARS-CoV-2 ORF7a (ORF7a Synthetic)
    

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPB-cT3G-cERP2-ZBTB16

    Plasmid

    #192903

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of ZBTB16

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    ZBTB16 (ZBTB16 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge