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pGMEH-T38M-750-luxAB-DAS+4

Plasmid

#49520

Purpose

Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomally

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

T38M-P750-luxAB-DAS+4

Tags

DAS+4 (AANDENYSENYADAS)

Expression

Bacterial

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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promoter J23110 (upstream of RBS, mRFP1)

Plasmid

#117852

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

medium-high promoter, RBS, mRFP1

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pMM9-MAPKK2-KW71 [delta(48-55)/S222D/S226D]

Plasmid

#40806

Depositor

Natalie Ahn

Article

Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

Insert

MAP2K2 (MAP2K2 Human)

Tags

HA

Expression

Mammalian

Mutation

delta(48-55) + S222D + S226D

Promoter

MSV-LTR

Available Since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
pLVXT-mTcf3

Plasmid

#121935

Purpose

doxycycline-inducible mouse Tcf3

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Transcription Factor 3 (Tcf3 Mouse)

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-Atoh1wt-IRES-RFP

Plasmid

#33334

Depositor

Martine Roussel, Charles Sherr

Article

Ayrault et al Cancer Res. 2010 Jul 1;70(13):5618-27. E

Insert

Atoh1 (Atoh1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
HOXA11 (murine) HIS-tag pET

Plasmid

#8517

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA11 (Hoxa11 Mouse)

Tags

T7

Expression

Bacterial

Mutation

makes protein by Western

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
mCardinal-NUP50-N-10

Plasmid

#56170

Purpose

Localization: Nuclear Pore, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

NUP50 (NUP50 Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pTYF-2xSYN-tTA

Plasmid

#19982

Depositor

Sergey Kasparov

Article

Liu et al J Gene Med. 2008 May . 10(5):583-92.

Insert

2xSYN-tTA (SYN1 Budding Yeast, Human, E.coli)

Expression

Mammalian

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

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HOXB2 (human) HIS-tag pET

Plasmid

#8522

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB2 (HOXB2 Human)

Tags

His and T7

Expression

Bacterial

Mutation

Starts at AA #2; makes protein by Western

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
WFS1 pEGFP-N2 (1472)

Plasmid

#62051

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

WFS1 (WFS1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pGEX Txk-SH2

Plasmid

#46526

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Txk (Txk Mouse)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 138-258

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_14

Plasmid

#194675

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_14 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_14

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_15

Plasmid

#194676

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_15 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_15

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pFSW Doc2alpha WT

Plasmid

#128812

Purpose

To make virus expressing Doc2alpha WT

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2alpha (Doc2a Rat)

Use

Lentiviral

Tags

IRES-EGFP

Promoter

hSyn (human synapsin I promoter)

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pFSW Doc2beta WT

Plasmid

#128814

Purpose

To make virus expressing Doc2beta WT

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Use

Lentiviral

Tags

IRES-EGFP

Promoter

hSyn (human synapsin I promoter)

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pSC008_pLKO-U6-BsmBI-chRNA(+85)-EFS-Thy11

Plasmid

#192147

Purpose

T cell CRISPR lentiviral vector

Depositor

Sidi Chen

Article

Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

Type

Empty backbone

Expression

Mammalian

Mutation

NA

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pSLIK-Venus-TmiR-Luc

Plasmid

#25739

Purpose

Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Lentiviral and RNAi

Tags

Venus

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ef1a-mCh-Puro-GFPi

Plasmid

#31848

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

GFP RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX-4T2 SHP2 E76A

Plasmid

#8324

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

E76A (activated mutant)

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only
pGEX Sh3bp2-SH2

Plasmid

#46484

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Sh3bp2 (SH3BP2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 450-561

Promoter

Tac

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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