We narrowed to 4,705 results for: golden gate
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Plasmid#231035PurposeCloning vector to clone promoter fragment in front of minimal p35S:Luciferase:tNOSDepositorInsertMinimal p35S:Luciferase:tNOS
UseLuciferaseExpressionBacterialPromoterminimal p35SAvailable SinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1643
Plasmid#229876Purposegene trap RMCE plasmid with Tet-On[rTetR-linker-QFAD]-act-4 3' UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCEDepositorInsertTet-On[rTetR-linker-QFAD]-act-4 3' UTR::SEC
ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1221
Plasmid#229873Purposegene trap RMCE plasmid with QF-act-4 3' UTR::SEC for RMCE cloned in the destination vector pHW1133 to swap driver via RMCEDepositorInsertQF-act-4 3' UTR::SEC
ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1177
Plasmid#229872Purposegene trap RMCE plasmid with Tet-Off[TetR-linker-QFAD]-act-4 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCEDepositorInsertTet-Off[TetR-QFAD]-act-4 3'UTR::SEC
ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
dSpyCas9-E566-GS-LOV2-VPR
Plasmid#231576PurposePotent light-deactivated CRISPRaDepositorInsertdSpyCas9-VPR with an AsLOV2 insertion behind E566
UseCRISPRExpressionMammalianAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-2xMS2
Plasmid#212627PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorInsertEF1a-PuroR-2xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-12xMS2
Plasmid#212628PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorInsertEF1a-PuroR-12xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB53
Plasmid#226309PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with V(T/S)G to AAA CsoS2 MR1-3
ExpressionBacterialMutationVTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…PromoterpTRCAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1559
Plasmid#218590PurposeExpress peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol with cytosolic Erg20 homodimerDepositorInsertGES
TagsePTS1ExpressionYeastMutationErg20(F96W, N127W), Erg20-Erg20 synthetic homodim…Available SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
TL1 alphaDdCBE T2 (DddA6-N)
Plasmid#221995PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233A | DddA: Q1310R, S1330I,…PromoterCMVAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only