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Showing: 7461 - 7480 of 25548 results
  1. pT7-agrBD-IV

    Plasmid
    #53440
    Purpose
    Derivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IV
    Depositor
    Insert
    agrBD locus (with D29Y mutation in agrD)
    Use
    Synthetic Biology
    Tags
    V5 epitope, 6x HIS (not expressed on agrB or agrD…
    Expression
    Bacterial
    Mutation
    D to Y mutation of residue 29 of the agrD gene (c…
    Promoter
    T7-lac
    Available Since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
  2. PatoM-LOG241

    Plasmid
    #72847
    Purpose
    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. LOG241 backbone.
    Depositor
    Insert
    PatoM
    Expression
    Bacterial
    Promoter
    PatoM
    Available Since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
  3. pBB0323-TEV-His12

    Plasmid
    #137039
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12
    Depositor
    Insert
    AAC66700.1
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pEGFP-PH-SWAP70(R223E, R224E)

    Plasmid
    #84583
    Purpose
    Expresses GFP-tagged PH-domain of SWAP70 in mammalian cells, impaired phosphoinositide binding
    Insert
    SWAP70 residues 209-306 with mutations R223E and R224E (SWAP70 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Pleckstrin-homology domain, residues 209-306, mut…
    Promoter
    CMV
    Available Since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
  5. pDEST-EGFP-Cx43ML-N1

    Plasmid
    #49860
    Purpose
    Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal EGFP fusion tag
    Depositor
    Insert
    Connexin 43 (GJA1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    All internal Methionines mutated to Leucine. No s…
    Promoter
    CMV
    Available Since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 7461 - 7480 of 25548 results