We narrowed to 19,524 results for: ACE;

1. pMP89b CvTA V124N

   Plasmid

   #141207

   Purpose

   Chromobacterium violaceum transaminase with enhances affinity for PLP. TEV cleaving site after the N-terminal Histag.

   Depositor

   Francesca Paradisi

   Article

   Roura Padrosa et al Front Bioeng Biotechnol. 2019 Oct 18;7:2

   Insert

   CvTA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V124N

   Promoter

   Available since

   June 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcF-2

   Plasmid

   #131025

   Purpose

   Single spacer array with spacer #2 for FnCas12a.

   Depositor

   Chase Beisel

   Article

   Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

   Insert

   single spacer array for FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23119

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcF-3

   Plasmid

   #131024

   Purpose

   Single spacer array with spacer #3 for FnCas12a.

   Depositor

   Chase Beisel

   Article

   Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

   Insert

   single spacer array for FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23119

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. shCDK4(1)

   Plasmid

   #73550

   Purpose

   shRNA(1) against CDK4

   Depositor

   Gerardo Ferbeyre

   Article

   Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

   Insert

   shRNA against CDK4
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

5. shCDK4(2)

   Plasmid

   #73551

   Purpose

   shRNA(2) against CDK4

   Depositor

   Gerardo Ferbeyre

   Article

   Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

   Insert

   shRNA against CDK4
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pJexpress414_OmpA171_WTnoGirdles

   Plasmid

   #62653

   Purpose

   Wild-type OmpA with aromatic girdle residues replaced by leucine

   Depositor

   Timothy Whitehead

   Article

   Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

   Insert

   OmpA_noGirdles
   

   Use

   Synthetic Biology

   Tags

   6His and FlAsH

   Expression

   Bacterial

   Mutation

   Aromatic girdle residues mutated to Leu (W7, W15,…

   Promoter

   T7

   Available since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pAKW.114A-PKA

   Plasmid

   #154051

   Purpose

   Expresses PKA in E. coli

   Depositor

   Christopher Voigt

   Article

   Wallace et al Advanced Functional Materials 2020

   Insert

   Protein kinase cAMP-activated catalytic subunit alpha (PKA) (PRKACA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pCin

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. alpha7nACHR_Nacho_GCAMP7s

   Plasmid

   #182940

   Purpose

   alpha7 nACHR expression in mammalian cell lines with calcium reporter

   Depositor

   Robert Brenner

   Article

   Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

   Insert

   NACH7 (CHRNA7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-αTAT1[2-236]

   Plasmid

   #27101

   Depositor

   Maxence Nachury

   Article

   Shida et al Proc Natl Acad Sci U S A. 2010 Nov 10. (

   Insert

   αTubulin K40 acetyltransferase (ATAT1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pZac2.1-GfaABC1D-TurboID(full)-HA-GPI

    Plasmid

    #166055

    Purpose

    Expresses the full length TurboID on the surface of astrocytes

    Depositor

    Scott Soderling

    Article

    Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

    Insert

    GPI anchored TurboID
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Plasmid EUPp_09

    Plasmid

    #157745

    Purpose

    PgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)

    Depositor

    Kang Zhou

    Article

    Liang et al Metab Eng. 2020 Nov 25. pii: S1096-7176(

    Inserts

    acetaldehyde dehydrogenase
    alcohol dehydrogenase (ADH2 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PgyrA

    Available since

    June 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. HDAC5 Flag

    Plasmid

    #13822

    Purpose

    Mammalian expression of human histone deacetylase 5 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC5 (HDAC5 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV-hSynI-V5-TurboID(N)-GPI

    Plasmid

    #163696

    Purpose

    Expresses n-terminal half of TurboID on surface of neurons

    Depositor

    Scott Soderling

    Article

    Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

    Insert

    GPI anchored TurboID
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pZac2.1-GfaABC1D-TurboID(C )-HA-GPI

    Plasmid

    #163697

    Purpose

    Expresses c-terminal half of TurboID on surface of astrocytes

    Depositor

    Scott Soderling

    Article

    Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

    Insert

    GPI anchored TurboID
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mbYFPQS

    Plasmid

    #80742

    Purpose

    membrane-targeted biosensor for monitoring changes in intracellular chloride

    Depositor

    Susan Amara, Susan Ingram

    Article

    Watts et al PLoS One. 2012;7(4):e35373. doi: 10.1371

    Insert

    YFPQS
    

    Use

    Tags

    palmitoylation signal sequence of neuromodulin

    Expression

    Mammalian

    Mutation

    H148Q (CAC to CAG) and V163S (GTG to AGC).

    Promoter

    CMV

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pNICKclos1.0

    Plasmid

    #73639

    Purpose

    Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

    Inserts

    Cas9 nickase
    sGRNA to pyrE
    

    Use

    E.coli-clostridium shuttle vector

    Tags

    Expression

    Mutation

    D10A

    Promoter

    j23119 and ptb

    Available since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAEMXT-eLACCO1.1

    Plasmid

    #167946

    Purpose

    Fluorescent biosensor for extracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

    Insert

    CD59-eLACCO1.1-GPI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CAG-eLACCO1.1

    Plasmid

    #168789

    Purpose

    Fluorescent sensor for extracellular lactate

    Depositor

    Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

    Insert

    eLACCO1.1
    

    Use

    AAV

    Tags

    ER export and GPI

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SQT1665

    Plasmid

    #78744

    Purpose

    Human expression plasmid for LbCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pdCASclos

    Plasmid

    #73640

    Purpose

    Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

    Inserts

    dCas9
    sgRNA to spo0A
    

    Use

    E.coli-clostridium shuttle vector

    Tags

    Expression

    Mutation

    D10A, H840A

    Promoter

    Pj23119 and ptb

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-hSyn-eLACCO1.1

    Plasmid

    #168788

    Purpose

    Neuronal expression of fluorescent sensor for extracellular lactate

    Depositor

    Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

    Insert

    eLACCO1.1
    

    Use

    AAV

    Tags

    ER export and GPI

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pATLIC

    Plasmid

    #110287

    Purpose

    A backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)

    Depositor

    In-Geol Choi

    Article

    Ko et al Appl Environ Microbiol. 2012 May;78(9):3

    Insert

    yfaL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pT7-SpCas9_sgRNA-site1 (RTW443)

    Plasmid

    #160136

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. IMS-HyPer2

    Plasmid

    #60248

    Purpose

    Genetically-encoded fluorescent hydrogen peroxide indicator HyPer2 targeted to mitochondrial intermembrane spase.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    IMS-HyPer2
    

    Use

    Tags

    Mitochondrial intermembrane space targeting signa…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. His-NavMs

    Plasmid

    #100004

    Purpose

    Electrophysiology in mammalian cells: Mammalian expression of voltage gated sodium channel and GFP

    Depositor

    Paul DeCaen

    Article

    Sula et al Nat Commun. 2017 Feb 16;8:14205. doi: 10

    Insert

    His-NavMs, Voltage gated sodium channel
    

    Use

    Tags

    Hexa-His tag followed by a thrombin cleavage site

    Expression

    Mammalian

    Mutation

    C-terminus from a codon-optimized synthetic gene,…

    Promoter

    CMV

    Available since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYpet-PBD

    Plasmid

    #22781

    Depositor

    Klaus Hahn

    Article

    Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

    Insert

    PAK binding domain (PAK1 Human)
    

    Use

    Tags

    Ypet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCyPet-Rac1(WT)

    Plasmid

    #22782

    Depositor

    Klaus Hahn

    Article

    Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

    Insert

    Rac1 (wt) (RAC1 Human)
    

    Use

    Tags

    CyPet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

28. pAAV-GFAP-eLACCO1.1

    Plasmid

    #168790

    Purpose

    Astrocyte expression of fluorescent sensor for extracellular lactate

    Depositor

    Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

    Insert

    eLacco1.1
    

    Use

    AAV

    Tags

    ER export and GPI

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. shCDK4/6(1)

    Plasmid

    #73554

    Purpose

    vector encoding both shRNA against CDK4(1) and shRNA against CDK6(1)

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shRNA against CDK4 + shRNA against CDK6
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

30. shCDK4/6(2)

    Plasmid

    #73555

    Purpose

    vector encoding both shRNA against CDK4(2) and shRNA against CDK6(2)

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shRNA against CDK4 + shRNA against CDK6
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only