We narrowed to 23,709 results for: CRISPR

Showing: 11221 - 11250 of 23709 results

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6xHis-MBP-huErCas12a

Plasmid

#174685

Purpose

Expresses 6xHis-MBP tagged ErCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huErCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV, NLS and NLS, 6xHis

Expression

Bacterial

Mutation

Q926R mutation is observed as indicated from the …

Promoter

T7

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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p206_LTJ_sgRNACD45.2_R3

Plasmid

#82674

Purpose

sgRNA targeting murine CD45.2 region 3. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting CD45.2 region 3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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M-NM-sgRNA

Plasmid

#48673

Purpose

Mammalian U6-driven sgRNA (NMm1) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with N. meningitidis Cas9, hU6 promoter

Use

CRISPR

Promoter

hU6

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-miniCMV-TdTomato

Plasmid

#192656

Purpose

A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

TdTomato

Expression

Mammalian

Promoter

U6, miniCMV

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pIKU801-CrPEPC1

Plasmid

#92342

Purpose

Expresses sgRNA for targeting CrPEPC1 gene, paromomycin resistance

Depositor

I-Son Ng

Article

Kao et al Bioresour Technol. 2017 May 4. pii: S096

Insert

sgRNA-CrPEPC1

Use

CRISPR

Expression

Plant

Promoter

AtU6-26

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFS_0235-pET30-Fusicatenibacter_saccharivorans_ARRAY2_FaqI

Plasmid

#116954

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pKM197

Plasmid

#132665

Purpose

pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.

Depositor

Joseph Sorg

Article

Bhattacharjee et al mSphere. 2020 Mar 4;5(2):e00941-19. doi:

Insert

Upstream & Downstream pyrE deletion region

Use

E. coli - c. difficile shuttle vector

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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M-ST1cas

Plasmid

#48669

Purpose

Mammalian S. thermophilus #1 Cas9 expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9

Use

CRISPR

Tags

NLS

Promoter

CMV

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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AAV-Stuffer

Plasmid

#106248

Purpose

Destination vector for U6::gRNA expression cassette

Depositor

Charles Gersbach

Article

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Type

Empty backbone

Use

AAV

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEX128·gRNA

Plasmid

#187600

Purpose

Template for amplification of gRNA with Cas6 recognition site

Depositor

Pablo Ivan Nikel

Article

Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

Insert

gRNA template

Promoter

no promoter

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMM704

Plasmid

#68891

Purpose

IPTG-inducible CRISPRi vector targeting BT1854, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pMB1610_pRR-Puro

Plasmid

#65853

Purpose

Puromycin-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9

Depositor

Marc Bühler

Article

Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:

Insert

split puromycin N-acetyltransferase coding sequence

Use

CRISPR and TALEN

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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U6-(BbsI)sgRNA_CAG-Venus-bpA

Plasmid

#86985

Purpose

For cloning and expression of sgRNA together with Venus expression

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Venus

Expression

Mammalian

Mutation

Venus GFP variant

Promoter

CAG

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pGL193

Plasmid

#180471

Purpose

For expression of Cas9 and gRNAs, multiple gRNAs cloning vector

Depositor

Peggy Lemaux

Article

Aregawi et al Plant Biotechnol J. 2021 Nov 26. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Cas9

Expression

Plant

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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px458_Conc2

Plasmid

#134449

Purpose

Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFP

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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M-NMcas

Plasmid

#48670

Purpose

Mammalian N. meningitidis Cas9 expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9

Use

CRISPR

Tags

NLS

Promoter

CMV

Available Since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only
pHK352.3

Plasmid

#235473

Purpose

Message phagemid carrying sgRNA3 (prom. J23110, backbone RSF1030)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA3

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pdCas9-NED

Plasmid

#109358

Purpose

Mammalian vector for expressing effector domains fused to dCas9.

Depositor

Antal Kiss

Article

Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

Type

Empty backbone

Use

CRISPR

Tags

dCas9, SV40 NLS, 3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pKM808

Plasmid

#134181

Purpose

Lentiviral sgRNA plasmid with blasticidin resistance

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCFD3-frame_selector_0

Plasmid

#127553

Purpose

Drosophila expression of frame selector sgRNA 0 that targets CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sgRNA frame 0

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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px458_Conc3

Plasmid

#134450

Purpose

Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + GFP

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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4 gRNA concatemer

Plasmid

#84881

Purpose

Empty concatmer vector in which 4 gRNAs can be inserted

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCFD3-frame_selector_1

Plasmid

#127554

Purpose

Drosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sgRNA frame 1

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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BB1_23_dCAS9(3xFLAG_SV40NLS)

Plasmid

#161478

Purpose

BB1 encoding a FLAG tagged dCAS9 with a SV40 NLS

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Insert

dCAS9

Use

CRISPR and Synthetic Biology

Tags

FLAG Tag, SV40 NLS

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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px459V2.0_Conc3

Plasmid

#134458

Purpose

Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + Puro

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

Plasmid

#128749

Purpose

Expresses control gRNA

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO1808_arC9_2xNLS_human

Plasmid

#74491

Purpose

Human codon optimized plasmid to express arC9 t2a mCherry w/2xNLS and a sgRNA

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

arC9

Tags

t2a mCherry

Expression

Mammalian

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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3xFLAG-dCas9/pTEF1p-CYC1t

Plasmid

#62190

Purpose

Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

Insert

3xFLAG-dCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Yeast

Mutation

human codon-optimized, D10A, H840A

Promoter

TEF1 promoter

Available Since

Feb. 24, 2015

Availability

Academic Institutions and Nonprofits only

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Cmv d0 dPspCas13b-GGS-Sun

Plasmid

#119858

Purpose

Cmv d0 dPspCas13b-GGS-Sun no-fusion construct

Depositor

Bryan Dickinson

Article

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Insert

dCas13b-GGS-Sun

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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DS-NMcas

Plasmid

#48646

Purpose

Bacterial N. meningitidis Cas9 (NM) + tracrRNA expression, cloDF13/spectinomycin

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
NM tracrRNA

Use

CRISPR

Promoter

NM tracrRNA promoter and proC

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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