We narrowed to 7,609 results for: Pol
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Plasmid#204227PurposeEpisomal yeast plasmid expressing the Ercas12aDepositorInsertEubacterium rectale cas12a (Ercas12a)
ExpressionYeastMutationcodon optimized for S. cerevisiaePromoterSaccharomyces cerevisiae PGK1Available SinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-iCre-pA]-lox-2xHA-128-425-Akap9
Plasmid#196897PurposeNeuron-specific expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to 2xHA-tags.DepositorAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)
Plasmid#169163PurposeBaculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cellsDepositorInsert3xFlag-6His-nsp14 (ORF1ab Synthetic, SARS-CoV-2)
Tags3xFlag-6HisExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3486 (YCp LEU2 Rpb1 F1492A)
Plasmid#91808PurposeYeast expression of S. cerevisiae Rpb1 with a F1492A mutationDepositorInsertRPO21 (RPO21 Budding Yeast)
ExpressionYeastMutationchanged phenylalanine 1492 to alaninePromoterEndogenous Rpb1 promoterAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)
Plasmid#91818PurposeBacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutationsDepositorInsertSPT6 (SPT6 Budding Yeast)
Tags6xHisExpressionBacterialMutationchanged lysine 1355 and lysine 1435 to alanines; …PromoterT7Available SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgRNA targeting human SLC12A5 gene stop codon
Plasmid#158577PurposeFor generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.DepositorInsertsgRNA targeting human SLC12A5 gene stop codon
UseCRISPRTagsCas9 and GFPExpressionMammalianAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
JARID1BA-c539
Plasmid#79643PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertJARID1B (KDM5B Human)
UseBaculovirus expressionTags6x His and TEV cleavage sitePromoterpolyhedrinAvailable SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET23a-MmUBB(SnaBI)
Plasmid#69558PurposeIn vitro transcription/translation (T7)DepositorInsertUBB (Ubb Mouse)
ExpressionBacterialMutationThe two last codons (TATTAA; encoding the last ty…PromoterT7Available SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT-5th R
Plasmid#42572DepositorInsertmutated calmodulin gene
TagsPolyhistidine (6×His) region, TEV recognition sit…ExpressionBacterialMutationchanged Phenylalanine 92 to Glutamine acid, Isole…PromoterT7Available SinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
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Tf_b.45_h90_t.5_i4400_11_45_ICCG
Plasmid#257175PurposeExpression construct for Tf_b.45_h90_t.5_i4400_11_45_ICCG gene, codon optimized for expression in E. coliDepositorInsertTf_b.45_h90_t.5_i4400_11_45_ICCG
ExpressionBacterialMutationR18S, S19R, E27T, R46T, E47S, N48T, N49G, Y51F, A…Available SinceJuly 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
Tf_b.45_h90_t.5_i4400_14_88_ICCG
Plasmid#257172PurposeExpression construct for Tf_b.45_h90_t.5_i4400_14_88_ICCG gene, codon optimized for expression in E. coliDepositorInsertTf_b.45_h90_t.5_i4400_14_88_ICCG
ExpressionBacterialMutationR18T, S19R, E27T, N28T, R46T, E47S, N48T, N49G, E…Available SinceJuly 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
Tf_b.45_h90_t.33_i2200_12_1_ICCG
Plasmid#257162PurposeExpression construct for Tf_b.45_h90_t.33_i2200_12_1_ICCG gene, codon optimized for expression in E. coliDepositorInsertTf_b.45_h90_t.33_i2200_12_1_ICCG
ExpressionBacterialMutationR18T, S19R, S25G, R46T, E47D, N49G, Y51F, A65T, E…Available SinceJuly 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-GP-TFIIB-2xStrep
Plasmid#213919PurposeSleeping beauty vector for expression of C-terminally strep-tagged TFIIBDepositorInsertTFIIB-2xStrep (GTF2B Human)
Tags2xStrepExpressionMammalianMutationCoding region changed for resistance to Dharmaco…PromoterEF-1EF1a/RPBSAAvailable SinceJune 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pGS-BacA-21222-SNF5
Plasmid#109186PurposePlasmid for expression of C-terminally 3xFLAG-tagged SNF5 under the control of a polh promoter in baculovirus/insect cell systemsDepositorAvailable SinceJuly 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFB-D3-ASK1
Plasmid#226619PurposeCo-expression of D3 and ASK1 complex in insect cellsDepositorInsertsTagsHis tag followed with three copies of MsybExpressionInsectMutationThe flexible loop in D3 (Q478-D515) can be remove…PromoterPolyhedrinAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGI_Myr-YFP-MiniTurboID
Plasmid#222432PurposePotential plasma membrane control.DepositorInsertMiniTurboID (BirA mutant)
UseGolden gate / green gate compatible cloning vectorTagsLinker, Myr, and YFPMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Available SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only