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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 19,524 results for: ACE;

Showing: 11251 - 11280 of 19524 results

  1. pT7-AsCas12a_crRNA-site4 (MSP3511)

    Plasmid
    #160139
    Purpose
    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Insert
    AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
    Use
    In vitro transcription of sgrna from t7 promoter
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    160139-plasmid-map-sequence-id-333510

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  4. pCH6 - pBait-ChiX

    Plasmid
    #174662
    Purpose
    arabinose-induced synthesis of a hybrid RNA containing a single MS2 hairpin followed by E coli ChiX inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.
    Depositor
    Katherine Berry
    Article
    Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507
    Insert
    ChiX
    Use
    Tags
    1xMS2 hairpin
    Expression
    Bacterial
    Mutation
    Promoter
    pBad
    Available since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    174662-plasmid-map-sequence-id-361921

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  6. shNTC

    Plasmid
    #73549
    Purpose
    Non-targeting control shRNA cloned in retroviral vector pMLP (MSCV-based vector expressing shRNA in a mir30 context).
    Depositor
    Gerardo Ferbeyre
    Article
    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d
    Insert
    shNTC
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    LTR
    Available since
    Aug. 8, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. pONSY

    Plasmid
    #111873
    Purpose
    "Capsaspora owczarzaki" expression vector (backbone) modified from the pCR2.1. It bears the promoter and terminator regions from the endogenous Elongation Factor 1 alpha (EF1a) gene (CAOG_07807).
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Type
    Empty backbone
    Use
    Capsaspora owczarzaki
    Tags
    Expression
    Mutation
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    111873-plasmid-map-sequence-id-217304

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  18. pWL502-PAM

    Plasmid
    #174382
    Purpose
    Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei
    Depositor
    Jing Han
    Article
    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:
    Insert
    PAM-spacer
    Use
    CRISPR; Archaeal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    174382-plasmid-map-sequence-id-344136

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  19. pT7-AsCas12a_crRNA-site3 (RTW549)

    Plasmid
    #160138
    Purpose
    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Insert
    AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)
    Use
    In vitro transcription of sgrna from t7 promoter
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    160138-plasmid-map-sequence-id-333508

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  25. pro::EX1-2(intΔNACs):GFP

    Plasmid
    #218570
    Purpose
    Transcriptional reporter for ARF7 promoter
    Depositor
    Anthony Bishopp
    Insert
    AT5G20730 (NPH4 Mustard Weed)
    Use
    Tags
    Expression
    Plant
    Mutation
    Mutation genomic sequence 85nt, 86nt from TA to C…
    Promoter
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    218570-plasmid-map-sequence-id-438438

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  26. pMM4_14

    Plasmid
    #183062
    Purpose
    Plasmid expressing a biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under RET2p and mCherry under HREsYGP1p with flanks for genome integration into HO locus
    Depositor
    Yvonne Nygård
    Article
    Mormino et al Microb Cell Fact. 2022 Oct 15;21(1):214.
    Inserts
    mCherry
    BM3R1-HAA1-mTurquoise2
    Use
    Synthetic Biology
    Tags
    mCherry and mTurquoise2
    Expression
    Yeast
    Mutation
    Promoter
    HREsYGP1p and RET2
    Available since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183062-plasmid-map-sequence-id-363407

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  27. pIALD2S

    Plasmid
    #185865
    Purpose
    Knocking out ALD6 and expressing anacetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP ) in Saccharomyces cerevisiae
    Depositor
    Claudia Vickers
    Article
    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2
    Insert
    ALD6 (-125, 40)> PADH1> Lr.pduP> TPDC1-ALD6 (1054, 1749)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185865-plasmid-map-sequence-id-370809

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  29. pCm N-term MBP SMT3 NikJC199U

    Plasmid
    #174363
    Purpose
    Expression plasmid with a ULP1 removable N-term MBP SUMO tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.
    Depositor
    Brandon Greene
    Article
    Caceres et al Methods Enzymol. 2022;662:119-141. doi:
    Insert
    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    Use
    Tags
    8xHis, MBP, SMT3, and TEV
    Expression
    Bacterial
    Mutation
    C199U
    Promoter
    T7
    Available since
    Dec. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174363-plasmid-map-sequence-id-342843

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Showing: 11251 - 11280 of 19524 results