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pCMV-wtTnsC

Plasmid

#234732

Purpose

Wild-type human-codon optimized PseTnsC in mammalian cells

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

PseTnsC

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-wtTnsAB

Plasmid

#234731

Purpose

Wild-type human-codon optimized PseTnsAB in mammalian cells

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

PseTnsAB

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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Nme2Cas9_pCDest

Plasmid

#119923

Purpose

Nme2Cas9 CMV-driven mammalian expression plasmid

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

Nme2Cas9

Tags

NLS-3xHA-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3b-8XGliBS:EGFP

Plasmid

#84602

Purpose

Gli protein-dependent EGFP reporter for analysis of Hedgehog signaling

Depositor

James Chen

Article

Hyman et al Proc Natl Acad Sci U S A. 2009 Aug 18;10

Insert

eGFP

Expression

Mammalian

Promoter

8X Gli-binding site

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO Puro DEST (670-1)

Plasmid

#17293

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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WT GLUT1-FLAG

Plasmid

#200094

Purpose

WT human GLUT1 with a C-terminal 3xFLAG tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Blast DEST (706-1)

Plasmid

#17451

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry_P2AT2A_GFP-nb

Plasmid

#238232

Purpose

For overexpression of mCherry_P2AT2A_GFP-nb

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pBT281

Plasmid

#122611

Purpose

express evoAPOBEC1-BE4max in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoAPOBEC1-BE4max

Expression

Mammalian

Mutation

E4K H109N H122L D124N R154H A165S P201S F205S

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Puro DEST (w529-2)

Plasmid

#19068

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pZR071_SFFV-dCas9-mCherry-KRAB

Plasmid

#180264

Purpose

Lentiviral expression vector for dCas9-KRAB in mammalian cells

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

KRAB and mCherry

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMK381 (AAVS1 CMV-OsTIR1F74G)

Plasmid

#140536

Purpose

AAVS1 CMV-OsTIR1(F74G)

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CMV-OsTIR1(F74G)

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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YFP B1 in pcDNA3.1

Plasmid

#36397

Depositor

Narasimhan Gautam

Article

Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

Insert

G protein B1 (GNB1 Bovine)

Tags

YFP

Expression

Mammalian

Promoter

cmv

Available Since

April 4, 2013

Availability

Academic Institutions and Nonprofits only
pC014 - LwCas13a-msfGFP

Plasmid

#91902

Purpose

Expresses active LwCas13a for mammalian RNA targeting

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a

Use

CRISPR

Tags

NLS and msfGFP-NLS-3xHA

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

Plasmid

#180280

Purpose

The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI sgRNA (backbone) and BFP

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-RiboL1-jGCaMP8m

Plasmid

#192617

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RiboL1-jGCaMP8m

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-mito-mTagBFP2

Plasmid

#216130

Purpose

Lentiviral vector for expressing mTagBFP2 localizing to mitochondria.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

EF1a-COX8AmitochondrialLocalizationSignal-mTagBFP2-WPRE

Use

Lentiviral

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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Nrxn3b-Nav1.6-MKII-f-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16

Plasmid

#163031

Purpose

FLiCRE TF with fast kinetics for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

Nrxn3b-MKII-f-hLOV1-TEVcs-tTA

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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Nrxn3b-Nav1.6-MKII-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16

Plasmid

#163029

Purpose

FLiCRE TF for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

Nrxn3b-MKII-hLOV1-TEVcs-tTA

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-enCas9(D10A)-PolI3M

Plasmid

#249061

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to PolI3M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9-PolI3M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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