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LLP738_L2pV1_1I_Act2_FRT_OCS_s35S_Flp_lacZ

Plasmid

#192381

Purpose

To test if the 35S promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZ

Plasmid

#192379

Purpose

To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output (despite the presence of the lacZ endlinker).

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP734_L2pV1_1I_OCS-rLUC_F-NOS-FlpO

Plasmid

#192377

Purpose

To test if the NOS promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP735_L2pV1_1I_OCS-rLUC_F-TCTP-FlpO

Plasmid

#192378

Purpose

To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pEY83

Plasmid

#191078

Purpose

flp-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-8 (flp-8 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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EK0544 CMV-TO-mCherry-SG(s70587-dblstop)SG-EGFP (FLP-IN)

Plasmid

#191176

Purpose

Mammalian expression of EK0209 with a second stop codon.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

CMV-TO:mCherry:s70587:EGFP (double-stop)

Expression

Mammalian

Mutation

additional stop codon

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0546 SFFV-mCherry-SGc(GAPDH.s1-dblstop)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#191178

Purpose

Mammalian expression of EK0493 with second stop codon.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:GAPDH.s1:EGFP (double-stop)

Expression

Mammalian

Mutation

additional stop codon

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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NSK025 CMV-TO-EGFP:c.637A>G(N213D; CCA>CCG)-stop-t70587 (PB)

Plasmid

#191166

Purpose

Mammalian expression of a EGFP mutant N213D derived from EK0379.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

EGFP(N213D)

Expression

Mammalian

Mutation

c.637A>G(N213D; CCA>CCG)

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0312 pAAV-EF1a-DIO-EYFP-WPRE-HGHpA

Plasmid

#191148

Purpose

AAV expression of a Cre reporter with EYFP output

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

DIO-EYFP

Use

AAV

Expression

Mammalian

Mutation

WT

Promoter

hEF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0362 SFFV-mCherry-SG(Bdnf.s1L36+54@202)SG-EGFP (FLP-IN)

Plasmid

#191153

Purpose

Mammalian expression of a 90 bp (36+54) long sensor for murine Bdnf 3' UTR where the trigger sequence is split

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:Bdnf.s1L36+54:EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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