We narrowed to 60,203 results for: cloning

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pQTEV-PSMD11

Plasmid

#31333

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 (PSMD11 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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LentiV2-mU6-evopreQ1

Plasmid

#210190

Purpose

pegRNA/ngRNA library cloning

Depositor

Yin Shen

Article

Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

Type

Empty backbone

Use

Lentiviral

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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mouse Clock delta 19

Plasmid

#177312

Purpose

expression of mutant mouse clock

Depositor

Joseph Takahashi

Article

King et al Cell. 1997 May 16;89(4):641-53. doi: 10.

Insert

mouse clock delta 19

Expression

Mammalian

Mutation

deletion from original mClock gene (514-564aa)

Promoter

CMV

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcU6_3 sgRNA

Plasmid

#92359

Purpose

Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

chick U6.3 promoter and gRNA cloning cassette

Use

CRISPR

Expression

Mammalian

Promoter

chick U6.3

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAJB2a

Plasmid

#19523

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB2a (DNAJB2 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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PB-CT3G-CERP2-MG-IRES2-mNeonGreen

Plasmid

#175508

Purpose

All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G-mCMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBAtC

Plasmid

#78097

Purpose

Plant expression of Cas9 and gRNA empty backbone; Basta resistance

Depositor

Jin-Soo Kim

Article

Kim et al J Integr Plant Biol. 2016 Mar 4. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

NLS-HA

Expression

Plant

Promoter

U6

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pPV-EF1a-EGxxFP-iP-A

Plasmid

#204624

Purpose

Cloning vector for SSA vector (EGxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

Depositor

Akitsu Hotta

Article

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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hGip-R

Plasmid

#14942

Depositor

Bernard Thorens

Article

Gremlich et al Diabetes. 1995 Oct . 44(10):1202-8.

Insert

Gip-R (GIPR Human)

Expression

Mammalian

Mutation

Short form of the receptor

Available Since

June 7, 2007

Availability

Academic Institutions and Nonprofits only
pHAtC

Plasmid

#78098

Purpose

Plant expression of Cas9 and gRNA empty backbone; Hygro Resistance

Depositor

Jin-Soo Kim, Sang-Gyu Kim

Article

Kim et al J Integr Plant Biol. 2016 Mar 4. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

U6

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-B/c

Plasmid

#227962

Purpose

Entry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

B/c

Promoter

None

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19/N-eCFP

Plasmid

#183155

Purpose

Plant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eCFP

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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bdSUMO-TAT-GFP-HiBit

Plasmid

#170991

Purpose

For bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-TAT-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAJB2b

Plasmid

#19524

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB2b (DNAJB2 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PA-3x-FLAG-B/BRISBANE/60/2008

Plasmid

#187922

Purpose

Expresses C terminal 3xFLAG tagged PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase acidic protein

Tags

3xFLAG

Promoter

CMV Promoter

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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BPK1520_puroR

Plasmid

#173901

Purpose

SpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selection

Depositor

Ronald Cohn

Article

Erwood et al Genome Res. 2019 Dec;29(12):2010-2019. d

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV/U6

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PB1-3x-FLAG-B/BRISBANE/60/2008

Plasmid

#187918

Purpose

Expresses C terminal 3xFLAG tagged PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase basic protein 1

Tags

3xFLAG

Promoter

CMV Promoter

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19/N-mChe

Plasmid

#183159

Purpose

Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of mCherry.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP DNAJB9

Plasmid

#19505

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB9 (DNAJB9 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pPT-TAR C1

Plasmid

#141246

Purpose

Transformation Associated Recombination (TAR) cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1

Depositor

Bogumil Karas

Article

Cochrane et al Algal Research, 2020. 50, 102014

Insert

Mitochondrial Genome of Phaeodactylum tricornutum

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Mutation

Minor additional mutations described in manuscript

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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