We narrowed to 1,701 results for: GAD
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Plasmid#110187PurposeExpression of mouse MeCP2 (R111G) in mammalian cellsDepositorInsertMeCP2_e2 FL [R111G] (mouse cDNA) (Mecp2 Mouse)
UseTagsEGFPExpressionMammalianMutationArg 111 GlyPromoterCMVAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1_MeCP2(deltaN)
Plasmid#110189PurposeExpression of mouse MeCP2 (deltaN) in mammalian cellsDepositorInsertMeCP2_e2 delta N (mouse cDNA) _EGFP (Mecp2 Mouse)
UseTagsEGFPExpressionMammalianMutationdelta N terminusPromoterCMVAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
6xHsa.NFKB:Kaede
Plasmid#239992PurposeKaede driven by NFkB activity can be switched from green to red fluorescence by UV light exposure, enabling tracking of NFkB positive cellsDepositorInsertphoto convertible Kaede
UseTagsNAExpressionMutationPromotercfos minimal promoterAvailable sinceJune 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCJ136
Plasmid#162665PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cre-IRES-PuroR
Plasmid#30205PurposeLentiviral coexpression of Cre and puromycin from the human EF1a promoterDepositorInsertCre recombinase (cre Enterobacteria phage P1)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceApril 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 AIF 1-90 mCherry FRB
Plasmid#67530PurposeFor expression of mCherry fused FRB to the mitochondrial innermembraneDepositorInsertsAIF a.a. 1 - 90
mCherry
FRB
UseTagsAIF 1 - 90, FRB, and mCherryExpressionMammalianMutationT->LPromoterCMVAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA
Plasmid#107270PurposeExpresses Cas9 upon induction with doxicycline.DepositorInsertshSpCas9
rtTA
bGH polyA-SV40polyA
UseTags3xFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterCAG and TREAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1_MeCP2(deltaNIC)
Plasmid#110191PurposeExpression of mouse MeCP2 (deltaNIC) in mammalian cellsDepositorInsertMeCP2_e2 delta NIC (mouse cDNA) - EGFP (Mecp2 Mouse)
UseTagsEGFPExpressionMammalianMutationdelta N and C termini. Intervening region replace…PromoterCMVAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
OPEN Beta2-microglobulin
Plasmid#215657PurposeE. coli expression of human beta-2-microglobulin containing a cysteine mutation for disulfide linkage to mutant HLA.DepositorInsertOPEN Beta2-microglobulin (B2M Human)
UseTagsExpressionBacterialMutationHistidine 31 to CysteinePromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRha-ABE8e-SpCas9-NG
Plasmid#201190PurposeExpresses the base editor ABE8e-SpCas9-NG in bacterial cellsDepositorInsertABE8e-SpCas9-NG
UseTags8xHIS and BP-NLSExpressionBacterialMutationPromoterpRhaBADAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX MLKL(wt)--2A-mCherry-puro
Plasmid#231974PurposeTet inducible expression of DmrB-Mlkl with mCherry to induce WT Mlkl expressionDepositorInsertMLKL WT construct with bi-cistronic mCherry (Mlkl Mouse)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRDA_122
Plasmid#208095PurposePerturb-Seq lentiviral gRNA expression vector with a 3' appended capture sequence to enable direct capture of CRISPR gRNAs for scRNA-seqDepositorInsertPuromycin resistance
UseCRISPR and LentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCJ207
Plasmid#162680PurposepET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.DepositorInsertPutative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL2del
Plasmid#216742PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 2 (OL2) by examining the variant lacking OL2 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 2 (OL2) with the sequence of [LEDSGYMA…PromoterAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL4del
Plasmid#216744PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 4 (OL4) by examining the variant lacking OL4 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL5del
Plasmid#216745PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 5 (OL5) by examining the variant lacking OL5 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 5 (OL5) with the sequence of [ATFAA] i…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL2/4sub
Plasmid#216746PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL2 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL3/4sub
Plasmid#216747PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL3 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL5/4sub
Plasmid#216748PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL5 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCDF Bravo-PotH-OL1del
Plasmid#216749PurposeStudy the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.DepositorInsertpotH (potH E. coli (Bacteria))
UseTags10x HisExpressionBacterialMutationOuter loop 1 (OL1) with the sequence of [FKISLAEM…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCDF Bravo-PotH-OL2del
Plasmid#216750PurposeStudy the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 2 (OL2) by examining the variant lacking OL1 in its interaction with exogenous peptides.DepositorInsertpotH (potH E. coli (Bacteria))
UseTags10x HisExpressionBacterialMutationOuter loop 2 (OL2) with the sequence of [WMGILKNN…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCDF Bravo-PotH-OL3del
Plasmid#216751PurposeStudy the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 3 (OL3) by examining the variant lacking OL1 in its interaction with exogenous peptides.DepositorInsertpotH (potH E. coli (Bacteria))
UseTags10x HisExpressionBacterialMutationOuter loop 3 (OL3) with the sequence of [ELLGGPDS…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL1del
Plasmid#216741PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 1 (OL1) with the sequence of [INIGGALQ…PromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
B52_puro_gRNA_3418711_KCNB2
Plasmid#197558PurposegRNA targeting upstream region of KCNB2 (site 3418711, control for epigenetic targeting)DepositorInsertupstream KCNB2 promoter gRNA (Kcnb2 Rat)
UseGrna with puromycin selectionTagspuromycin resistance cassette under CMV promotionExpressionMammalianMutationPromoterU6Available sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-DmrB-DmrB-Ripk3-2A-mCherry-puro
Plasmid#231972PurposeTet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosisDepositorInsertRipk3-deltaC dimerizing construct with bi-cistronic mCherry (Ripk3 Mouse)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-DmrB-Casp8-2A-mCherry-puro
Plasmid#231971PurposeTet inducible expression of DmrB-Casp8 with mCherry to induce Casp8 apoptosisDepositorInsertCasp8 dimerizing construct with bi-cistronic mCherry (CASP8 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2
Plasmid#158653Purposemaking transgenics expressing a UAS driven mCherry and NTR 2.0DepositorInsert5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
UseTol2-based transgenic expression in zebrafish/oth…TagsExpressionMutationF70A/F108YPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-multi-CRISPR-sgCasp8_#2-puro
Plasmid#231978PurposeKnockout mouse Casp8DepositorInsertsgRNA with Cas9 with puromycin resistance (Casp8 Mouse)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-multi-CRISPR-sgCasp8_#1-puro
Plasmid#231977PurposeKnockout mouse Casp8DepositorInsertsgRNA with Cas9 with puromycin resistance (Casp8 Mouse)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
plx304-PHB-V5
Plasmid#122230PurposeExpresses human PHB attached to a V5 tagDepositorInsertProhibitin (PHB1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:ECFP
Plasmid#158654Purposemaking transgenics expressing a QUAS driven YFP and NTR 2.0DepositorInsert5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:eCFP
UseTol2-based transgenic expression in zebrafish/oth…TagsExpressionMutationF70A/F108YPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2
Plasmid#158652Purposemaking transgenics expressing a UAS driven eGFP and NTR 2.0DepositorInsert5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
UseTol2-based transgenic expression in zebrafish/oth…TagsExpressionMutationF70A/F108YPromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2
Plasmid#158651Purposemaking transgenics expressing a UAS driven YFP and NTR 2.0DepositorInsert5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
UseTol2-based transgenic expression in zebrafish/oth…TagsExpressionMutationF70A/F108YPromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCJ202
Plasmid#162675PurposepET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.DepositorInsertPETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
UseTagsHisExpressionBacterialMutationW159C, S238C; codon optimized for expression in E…PromoterT7/lacAvailable sinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ199
Plasmid#162672PurposepET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with C-terminal His tag, codon optimized for expression in E. coli K12.DepositorInsertPutative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with signal peptide
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ197
Plasmid#162670PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S131G mutation.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1
UseTagsHisExpressionBacterialMutationS131G; codon optimized for expression in E. coli …PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ203
Plasmid#162678PurposepET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)DepositorInsertPutative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
UseTagsHisExpressionBacterialMutationdeltaF2-A75; codon optimized for expression in E.…PromoterT7/lacAvailable sinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CRISPRv2-sgIl1r1_#2-hygro
Plasmid#231990PurposeKnockout mouse Il1r1DepositorInsertsgRNA with Cas9 and hygromycin resistance (Il1r1 Mouse)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-multi-CRISPR-sgRipk3_#2-puro
Plasmid#231982PurposeKnockout mouse Ripk3DepositorInsertsgRNA with Cas9 with puromycin resistance (Ripk3 Mouse)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-multi-CRISPR-sgRipk3_#1-puro
Plasmid#231981PurposeKnockout mouse Ripk3DepositorInsertsgRNA with Cas9 with puromycin resistance (Ripk3 Mouse)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only