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Showing: 1 - 20 of 1677 results

  2. Anti-GAD65/GAD67 [L127/12-rat IgG2a] - Chimeric

    Plasmid
    #227046
    Purpose
    Mammalian expression plasmid of anti-GAD65/GAD67 (Homo sapiens) rat IgG2a R-mAb. Derived from hybridoma L127/12.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-GAD65/GAD67 (Homo sapiens) recombinant mouse monoclonal antibody (GAD1, GAD2 Chimera: M.musculus (mouse)/R.norvegicus (rat))
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    227046-plasmid-map-sequence-id-459983

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  5. pLN484 (FuGW-S(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe)

    Plasmid
    #105192
    Purpose
    Module 1 - synthetic promoter cMYC drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information)
    Depositor
    Timothy Lu
    Article
    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.
    Insert
    S(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    105192-plasmid-map-sequence-id-201266

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  6. pLN552 (FuGW-S(USF1)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe)

    Plasmid
    #105202
    Purpose
    Module 1 - synthetic promoter USF1 drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information)
    Depositor
    Timothy Lu
    Article
    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.
    Insert
    S(USF1)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    105202-plasmid-map-sequence-id-201273

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  9. pBS_flGAD

    Plasmid
    #87031
    Purpose
    Expresses GAD (Glutamic Acid Decarboxylase) in Bacteria
    Depositor
    Janice Blum
    Insert
    flGAD (GAD2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Lac-Z
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    87031-plasmid-map-sequence-id-173237

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  13. pGADT7-δ

    Plasmid
    #197259
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-3 δ fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Insert
    AP-3 δ (AP3D1 Human)
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Val 549 Ile substitution; silent substitutions in…
    Promoter
    ADH1
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197259-plasmid-map-sequence-id-387016

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  14. pGADT7-αC

    Plasmid
    #197650
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Insert
    AP-2 αC
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Ser677T substitution
    Promoter
    ADH1
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197650-plasmid-map-sequence-id-388876

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  15. pGADT7-AP-4 β4

    Plasmid
    #198327
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-4 β4 fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.
    Insert
    AP-4 β4
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Contains L480S substitution
    Promoter
    ADH1
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    198327-plasmid-map-sequence-id-387831

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  17. pGADT7-AP-2 σ2

    Plasmid
    #198345
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-2 σ2 fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.
    Insert
    AP-2 σ2
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    R42G substitution, and silent substitutions in co…
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198345-plasmid-map-sequence-id-387832

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  18. pGADT7-γ1

    Plasmid
    #197078
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 γ1 fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Insert
    AP-1 γ1 (Ap1g1 Mouse)
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197078-plasmid-map-sequence-id-387046

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  19. pGADT7-ε

    Plasmid
    #197261
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Insert
    AP-4 ε (AP4E1 Human)
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    silent substitutions in codons 905, 1129 and 1137
    Promoter
    ADH1
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197261-plasmid-map-sequence-id-387043

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Showing: 1 - 20 of 1677 results