We narrowed to 21,714 results for: ESC;

1. csr-1_son-1_sGFP

   Plasmid

   #191561

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Use

   Tags

   NCU04502 promoter and sGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. csr-1_son-1_mRuby3

   Plasmid

   #191744

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Use

   Tags

   NCU04502 promoter and mRuby3

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. csr-1_son-1_mNeptune2.5

   Plasmid

   #191748

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Use

   Tags

   NCU04502 promoter and mNeptune2.5

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5/FRT/TO_STf

   Plasmid

   #182000

   Purpose

   CMV overexpression of SNAP-tag fast in mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   STf
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO_STf_SKL

   Plasmid

   #182007

   Purpose

   CMV overexpression of SNAP-tag fast in the peroxisome of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   STf_SKL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBS-SKII-3XHA-eDHFR-Kan

   Plasmid

   #188928

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-eDHFR-Kan
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS-SKII-URA-GAL1-DHFR-3xHA

   Plasmid

   #188934

   Purpose

   Integration cassette to create N-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   URA-GAL1-DHFR-3xHA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pfMDH_K381A

   Plasmid

   #67732

   Purpose

   Expresses K381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Use

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed lysine 381 to alanine

   Promoter

   T7

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pfMDH_N300D

   Plasmid

   #67728

   Purpose

   Expresses N300D mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Use

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed asparagine 300 to aspartate

   Promoter

   T7

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pfMDH_R373A

    Plasmid

    #67731

    Purpose

    Expresses R373A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed arginine 373 to alanine

    Promoter

    T7

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pfMDH_N300S

    Plasmid

    #67729

    Purpose

    Expresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed asparagine 300 to serine

    Promoter

    T7

    Available since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCVD437

    Plasmid

    #63758

    Purpose

    7 Kb Bg1II I fragment of pCVD 436 cloned into BamHI site of pTTQ181 (incorrect orientation for expression off of pTTQ181 promoter)

    Depositor

    James Kaper

    Article

    Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20

    Insert

    eae
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCVD434

    Plasmid

    #63757

    Purpose

    EPEC eae probe. 1.1 kb Sa1I/StuI fragment of E2348/69 (from gene bank cosmid II E1) cloned into Sa1I/SmaI site of pUC18.

    Depositor

    James Kaper

    Article

    Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20

    Insert

    eae probe
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. mCerulean-Zyxin-6

    Plasmid

    #55398

    Purpose

    Localization: Focal Adhesions, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Zyxin (ZYX Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-smURFP-IRES-eGFP

    Plasmid

    #80343

    Purpose

    Expresses smURFP and eGFP in mammalian cells.

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    smURFP
    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and IRES

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQE30-mBFP

    Plasmid

    #112682

    Purpose

    Expression of NADPH-dependent blue fluorescent protein

    Depositor

    Geun-Joong Kim

    Article

    Hwang et al Biochem Biophys Res Commun. 2012 Mar 23;

    Insert

    His-mBFP
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMV306hsp+LuxG13

    Plasmid

    #26161

    Depositor

    Siouxsie Wiles, Brian Robertson

    Article

    Andreu et al PLoS One. 2010 May 24;5(5):e10777.

    Insert

    Bacterial luciferase operon + G13 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    It contains a gram-positive enhanced translation …

    Promoter

    Available since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. tdTomato-N1

    Plasmid

    #54642

    Purpose

    Localization: N1 Cloning Vector, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson, Nathan Shaner, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-MCN-10His-mStayGold (E138D)

    Plasmid

    #211361

    Purpose

    Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    mStayGold
    

    Use

    Tags

    10xHis-tag

    Expression

    Bacterial

    Mutation

    E138D

    Promoter

    T7

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EGFP-Tubulin-6

    Plasmid

    #56450

    Purpose

    Localization: Microtubules, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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