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pBTK552

Plasmid

#110618

Purpose

BTK assembled broad-host-range plasmid encoding inducible GFP driven CP25 promoter with lac operator. Also encodes LacI.

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK569

Plasmid

#110614

Purpose

BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by CP25 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

E2-Crimson

Use

Tags

Expression

Bacterial

Mutation

V32M (see comments below)

Promoter

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pDule2-IBBN (G2)

Plasmid

#85501

Purpose

Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

Depositor

Ryan Mehl

Article

Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

Insert

IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system

Use

Tags

Expression

Bacterial

Mutation

Y32G L65E F108W Q109M D158S R162K

Promoter

lpp (constitutive)

Available since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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LTN1_pCSdest

Plasmid

#53855

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

LTN1 (LTN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pKH162_FLAG-APEX2-YIPF3 LIR mut

Plasmid

#214981

Purpose

Flag-APEX2 tagged LIR mutant YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2-YIPF3_mut (YIPF3 Human)

Use

Lentiviral

Tags

Expression

Mutation

LIR mutant

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ROR1-V5/HIS

Plasmid

#205607

Purpose

Expression of human ROR1 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

ROR1 receptor tyrosine kinase (ROR1 Human)

Use

Tags

V5-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pQTEV-AIP

Plasmid

#31575

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

aryl hydrocarbon receptor interacting protein (AIP Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pDule-IBBN (G2)

Plasmid

#85500

Purpose

Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

Depositor

Ryan Mehl

Article

Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

Insert

IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system

Use

Tags

Expression

Bacterial

Mutation

Y32G L65E F108W Q109M D158S R162K

Promoter

lpp (constitutive)

Available since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pBTK601

Plasmid

#110607

Purpose

BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Cas9

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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BRWD1_pCSdest

Plasmid

#53894

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

BRWD1 (BRWD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
CMYA5T2/pDEST15

Plasmid

#98109

Purpose

Express CMYA5 (aa 4039 – 4069) Leu allele (4063) with a N-terminal GST tag in bacteria

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

CMYA5 (CMYA5 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

rs10043986 (NP_705838.3:p.Pro4063Leu)

Promoter

Available since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pYI2206

Plasmid

#228348

Purpose

Constitutive rPhlTA mutant expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

phlTA mutant

Use

Synthetic Biology

Tags

Three copies of VP16 (VP48)-Nuclear localization …

Expression

Yeast

Mutation

P5S, S6P, K86T, F109L, Q117R, E143K

Promoter

KpGAPDH promoter

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pMT478

Plasmid

#228286

Purpose

Constitutive mutant rPhlTA expression with strong promoter

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

rphlTA mutant

Use

Synthetic Biology

Tags

Three copies of VP16 (VP48)-Nuclear localization …

Expression

Yeast

Mutation

P5S, S6P, K86T, F109L, Q117R, E143K

Promoter

KpGAPDH promoter

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-flex-iGABASnFR2n-WPRE

Plasmid

#218881

Purpose

AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2n

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

S99A F104H R168P

Promoter

CAG

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE

Plasmid

#218873

Purpose

AAV-mediated expression of improved GABA sensor control (no change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Has Service

AAV1 and AAV5

Insert

iGABASnFR2(no bind)

Use

AAV

Tags

Expression

Mammalian

Mutation

S99A F102G R168P

Promoter

GFAP

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-iGABASnFR2(no bind)-WPRE

Plasmid

#218876

Purpose

AAV-mediated expression of improved GABA sensor control (no change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Has Service

AAV1 and AAV5

Insert

iGABASnFR2(no bind)

Use

AAV

Tags

Expression

Mammalian

Mutation

S99A F102G R168P

Promoter

Synapsin

Available since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRC2 CTF-FLAG

Plasmid

#217685

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

CELSR2 (CELSR2 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2356

Promoter

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRC2 CTFdeltaTA-FLAG

Plasmid

#217718

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

CELSR2 (CELSR2 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2362

Promoter

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pEG302-HD2C-ZF

Plasmid

#200917

Purpose

Express HD2C-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Article

Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

Insert

HD2C (HD2C Mustard Weed)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEG302-CPL2-ZF

Plasmid

#200918

Purpose

Express CPL2-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Article

Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

Insert

CPL2 (CPL2 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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