We narrowed to 159,258 results for: addgene

1. attB53_SNCB-_attB53

   Plasmid

   #183615

   Purpose

   RMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Inserts

   LaG17 GFP nanobody SynNotch tTA
   TRE-mCherry-SV40pA
   tagBFP
   

   Use

   Rmce

   Tags

   Myc and human CD8a signal sequence

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFUGW-shRIIα

   Plasmid

   #183454

   Purpose

   sgRNA targeting rat PKA-RIIα subunits

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   sgRNA targeting rat PKA-RIIα (Prkar2a Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFUGW-scrambled

   Plasmid

   #183455

   Purpose

   This control vector contains a scrambled version of the targeting sequence used in the pFUGW-shRIIα construct

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   scrambled sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330_gRNA_IG-DMR-1

   Plasmid

   #159931

   Purpose

   Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse IG-DMR
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330_gRNA_IG-DMR-2

   Plasmid

   #159932

   Purpose

   Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse IG-DMR
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLIK_hygro_p53(R280K)_V5

   Plasmid

   #136540

   Purpose

   Lentiviral expression vector for an inducible p53(R280K)-V5

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   p53(R280K) (TP53 Human)
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Tags

   V5

   Expression

   Mammalian

   Mutation

   p53(R280K)V5 was cloned from pLenti6-p53-R280K-V5…

   Promoter

   Available since

   June 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEN_TTmiRc2_p53(R280K)_V5

   Plasmid

   #136525

   Purpose

   Used as a donor vector to clone into pSLIK

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   p53(R280K) (TP53 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   V5

   Expression

   Mutation

   p53(R280K)V5 was cloned from pLenti6-p53-R280K-V5…

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Ef1a-fDIO-JRGECO1a

   Plasmid

   #128317

   Purpose

   Expresses JRGECO1a under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

   Depositor

   Patricia Jensen

   Has Service

   AAV1

   Insert

   NES-JRGECO1a
   

   Use

   AAV

   Tags

   His Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. meGFP-hTRAK1

   Plasmid

   #188664

   Purpose

   human TRAK1 with N-terminal meGFP tag

   Depositor

   Thomas Schwarz

   Insert

   OIP106 (TRAK1 Human)
   

   Use

   Tags

   meGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-BE4max-P2A-EGFP (pRZ33)

    Plasmid

    #140083

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP

    Depositor

    Keith Joung

    Insert

    Human codon optimized pCAG BE4max with P2A-EGFP
    

    Use

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A (SpCas9)

    Promoter

    CAG

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

    Plasmid

    #154868

    Purpose

    Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry

    Depositor

    Ulrik Gether

    Has Service

    AAV Retrograde and AAV8

    Insert

    hM3D(Gq)-mCherry
    

    Use

    AAV; Flp-frt

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin-1

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUltra-Plus-H2B-mTagBFP2

    Plasmid

    #161790

    Purpose

    3rd generation lentiviral bicistronic vector for mammalian expression of H2B-mTagBFP2 and one gene of interest

    Depositor

    Jorge Luis Arias-Arias

    Insert

    H2B-mTagBFP2
    

    Use

    Lentiviral; Bicistronic

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    I174A in the mTagBFP2 protein according to PLoS O…

    Promoter

    UbC and UbC promoter

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. 7TFC

    Plasmid

    #24307

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    7xTcf-FFluc // SV40-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. PERK 6: PERK.9E10.pBABE

    Plasmid

    #21819

    Depositor

    David Ron

    Insert

    PERK (Eif2ak3 Mouse)
    

    Use

    Retroviral

    Tags

    Myc (9E10)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CMV-FLEX-SaCas9-U6-sgOprk1

    Plasmid

    #159903

    Purpose

    Mutagenesis of Oprk1

    Depositor

    Larry Zweifel

    Insert

    Oprk1 (Oprk1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)

    Plasmid

    #176932

    Purpose

    Donor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hGEM(1-110)_mNG_hSLBP(18-108)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK1

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CMV-FLEX-SaCas9-U6-sgNts

    Plasmid

    #159909

    Purpose

    Mutagenesis of Nts

    Depositor

    Larry Zweifel

    Insert

    Nts (Nts Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-HA_human_BAP1

    Plasmid

    #158109

    Purpose

    Expresses tagged BAP1, neomycin selection

    Depositor

    Emanuela Felley-Bosco

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. XLone-BSD SPI1 P2A mCherry

    Plasmid

    #140031

    Purpose

    Tunable and temporal expression control of SPI1 and mCherry

    Depositor

    Xiaoping Bao

    Insert

    SPI1 (SPI1 Human)
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSCV-Jarid1c-IRES-GFP

    Plasmid

    #166009

    Purpose

    Retroviral biscistronic expression of Jarid1c and eGFP

    Depositor

    Manu Rangachari

    Insert

    Jarid1c (Kdm5c Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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