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Plasmid#154048PurposeExpresses PKA in E. coliDepositorPublicationInsertProtein kinase cAMP-activated catalytic subunit alpha (PKA) (PRKACA Human)
TagsHis, MBPExpressionBacterialPromoterT7Available sinceDec. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDRM98 mKate2 Blast
Plasmid#174720PurposeExpresses the NLS-tagged mKate2 gene, P2A and the blasticidin resistance gene.DepositorInsertsmKate2
BSD
UseLentiviralTagsP2A linker and SV40 NLS with GGS linkerExpressionMammalianPromoterMND and MND (off mKate-P2A)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX311-GFP-MEKDD
Plasmid#194882PurposeExpression of dominant negative MEK1DepositorAvailable sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pICH-nuc-roGFP2-synORP1
Plasmid#191759PurposeTi plasmid pICH86988 harbouring nuc-roGFP2-synORP1 for H2O2 detection in the nuclei of plant cells.DepositorInsertnuc-roGFP2-synORP1
UseBinary agrobacterium ti plasmidExpressionPlantPromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-mRFP-; gcry1:BFP -1
Plasmid#173887PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-mRFP; gcry1: BFP
UseSynthetic BiologyAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p667-UBC-CDK12-V5-TurboID_IDG-K
Plasmid#135244PurposeGateway destination clone of CDK12 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertCDK12-V5-TurboID (CDK12 Human)
UseLentiviral; Gateway destinationTagsV5-TurboIDExpressionMammalianPromoterUbiquitinAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX302 ID2-V5 puro
Plasmid#83096PurposeLentiviral vector for constitutive expression of human ID2 with C-terminal V5 tagDepositorAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TET-pLKO.1 PURO shSMAD4 #2
Plasmid#83091PurposeLentiviral shRNA vector for inducible knockdown of human SMAD4DepositorAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR
Plasmid#108104PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation)PromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-AR-1-333
Plasmid#104194PurposeExpresses human AR aa 1-333 in pGEX-5x-1 bacteral expression vector with GSTDepositorAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
iMb-Notch-Mosaic (IR99.40)
Plasmid#99749PurposeRosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteins and Notch signalling genesDepositorInsertMbYFP, MbTomato, MbKate2, DN-Rbpj and NICD-PEST
UseCre/Lox and Mouse TargetingExpressionMammalianAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pN3FS-CYC1
Plasmid#85783PurposeN-ICE plasmid pH3FS-CYC1DepositorInsertloxP-NatMX-loxP-CYC1p-3FLAG
UseCre/LoxExpressionYeastMutation*(see below)Available sinceSept. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSico-shRaptor
Plasmid#81086Purposeconditional expression of an shRNA targeting murine RaptorDepositorInsertRaptor shRNA (Rptor Mouse)
UseCre/Lox, Lentiviral, and RNAiExpressionMammalianPromoterU6Available sinceSept. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB119
Plasmid#68573PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsModified EAR motif plant-specific repression doma…ExpressionPlantPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
Neo1.a-Fc-His
Plasmid#72089PurposeExpresses the extracellular region of the Neogenin 1, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable sinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
VRK1
Plasmid#39067PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertVRK1 (VRK1 Human)
TagsHis TevExpressionBacterialMutationSurface mutant for crystallisation K34A, K35A, E3…Available sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSYN-PC7-006
Plasmid#42502DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPC7-SOL CDS (PCSK7 Human)
ExpressionMammalianPromotercmvAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-S109A
Plasmid#33088DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertYAP (YAP1 )
TagsFlagExpressionMammalianMutationS109APromoterCMVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSCV-ERK5-AEF
Plasmid#31816DepositorInsertErk5 (Mapk7 Mouse)
UseRetroviralExpressionMammalianMutationT219A and Y221FPromoterPCMV-LTRAvailable sinceSept. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFB-HTB-ASK1
Plasmid#29517DepositorInsertASK1 (SKP1 Mustard Weed)
ExpressionInsectAvailable sinceApril 14, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by