We narrowed to 9,521 results for: try

1. pENTR-2B-Gata4

   Plasmid

   #98615

   Purpose

   Gateway entry vector for Gata4

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   Gata4 (Gata4 Mouse)
   

   Use

   Entry vector

   Available Since

   July 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKK-NoTag

   Plasmid

   #105767

   Purpose

   Control vector for pKK series (encodes only SLIC arms (TEV-L and TEV-R)); expression without tag. Useful to design other vectors; any tag can be added.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Expression

   Mammalian

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffold

   Plasmid

   #121841

   Purpose

   pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven SaCas9 gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to dCas9 w/ gRNA expression

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKK-FLAG-TEV

   Plasmid

   #105768

   Purpose

   Expression of your protein of interest in fusion with FLAG at the N-terminus. The tag is cleavable by TEV protease or enterokinase.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mCherry-hPMCA2xb

   Plasmid

   #47751

   Purpose

   mammalian expression of mCherry tagged hPMCA2, xb splice variant

   Depositor

   Emanuel Strehler

   Article

   Paszty et al Sci Signal. 2015 Feb 17;8(364):ra19. doi

   Insert

   PMCA2x/b (ATP2B2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   xb splice variant

   Promoter

   CMV

   Available Since

   Nov. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

6. pYPQ239-RR

   Plasmid

   #108862

   Purpose

   FnCpf1 Gateway entry plasmid with N623R and K687R mutations

   Depositor

   Yiping Qi

   Article

   Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

   Insert

   FnCpf1-RR (FnCpf1 with N623R and K687R mutations)
   

   Use

   CRISPR; Gateway compatible cpf1 entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   N623R and K687R, Cpf1 is rice codon optimized

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MO91-ORAI1N223A-TEV-pHluorin (ORAI1-EC-pHluorin)

   Plasmid

   #174336

   Purpose

   The ORAI1N223A mutant containing-TEV-pHluorin-TEV sequence in the extracellular loop 2

   Depositor

   Sonal Srikanth

   Article

   Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

   Insert

   human Orai1 (ORAI1 Human)
   

   Use

   Retroviral

   Tags

   pHluorin inserted in the extracellular loop 2 of …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LG002: pMAGIC (L1-R5) mU6::xCas9(3.7) gRNA scaffold

   Plasmid

   #121816

   Purpose

   pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKK-TEV-mEGFP

   Plasmid

   #105794

   Purpose

   Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-mEGFP

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRK5 beta4 1355T

    Plasmid

    #16038

    Depositor

    Filippo Giancotti

    Article

    Nikolopoulos et al Mol Cell Biol. 2005 Jul . 25(14):6090-10

    Insert

    beta4 (ITGB4 Human)
    

    Expression

    Mammalian

    Mutation

    1355T: amino acids 1-1355

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only