We narrowed to 60,203 results for: cloning
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Plasmid#44489DepositorInsertMinimal Mos1 [Peft-3 GFP H2B tbb-2 UTR, NeoR]
UseGateway expression cloneAvailable SinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF
Plasmid#72949PurposeEntry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction siteDepositorInsertLevel 0 - ORFs
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNP1 OR sfGFP
Plasmid#107364PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 OR input A and pNP1 OR input B are co-expressedDepositorInsertOR gate + sfGFP
ExpressionBacterialPromoterT7Available SinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
BB1_23_eGFP
Plasmid#98518PurposeBB1 with insert: CDS eGFPDepositorInserteGFP
ExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAPi
Plasmid#127548PurposeEmpty control for pGAPi-based RNAi silencing in tandem with APT transcript segmentDepositorInsertAdenine Phosphorybosyltransferase transcript segment
UseRNAi; Empty controlPromoterUbiquitinAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
Act5C-pDONR221
Plasmid#124242PurposeGateway entry clone of Drosophila Actin5C cDNA.DepositorInsertDrosophila Actin 5C (Act5C Fly)
ExpressionInsectAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
BB3aK_14*
Plasmid#98529Purposeempty BB3 for insertion and overexpression of 1 gene in P. pastorisDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pLAT1
Plasmid#98509PurposeBB1 with insert: promoter PLAT1DepositorInsertLAT1 promoter
ExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pPFK300
Plasmid#98510PurposeBB1 with insert: promoter PPpPfkDepositorInsertPpPfk promoter
ExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pDAS2
Plasmid#98513PurposeBB1 with insert: promoter PDAS2DepositorInsertDAS2 promoter
ExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
MCV-HF LTS220A/S239A
Plasmid#112191PurposeMerkel cell polyomavirus molecular cloneDepositorInsertMCV-HF LTS220A/S239A
MutationT to G mutations at 3313 (nt) and 3370 (nt) of fu…PromoterNone from vector. Insert contains native promoter…Available SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pRPL2A
Plasmid#98508PurposeBB1 with insert: promoter PRPL2ADepositorInsertRPL2A promoter
ExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
kank4_pJet1.2
Plasmid#121982Purposefor generating kank4 riboprobes for WISH in zebrafishDepositorAvailable SinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Rae1-pDONR221
Plasmid#124240PurposeGateway entry clone of Drosophila Rae1 cDNA.DepositorInsertDrosophila Rae1 (Rae1 Fly)
ExpressionInsectAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRDA_103
Plasmid#133460PurposeH1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.DepositorInsertcontrol guide
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pDAS1
Plasmid#98515PurposeBB1 with insert: promoter PDAS1DepositorInsertDAS1 promoter
ExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNP1 AND gate sfGFP
Plasmid#107361PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 AND input A and pNP1 AND input B are co-expressedDepositorInsertAND gate + sfGFP
ExpressionBacterialPromoterT7Available SinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag D16A+H588A
Plasmid#109033PurposeD16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
TagsFlag TagExpressionBacterialPromoterT7Available SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK025
Plasmid#105415PurposeSynthetic biologyDepositorInsertHormone-binding domain and regulatory sequences (estrogen receptor)
UseSynthetic BiologyMutationBsaI/ BpiI restriction sites removedAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB3rN_AH
Plasmid#98556Purposeempty BB3 for insertion and overexpression of 7 genes in P. pastorisDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only