We narrowed to 9,521 results for: try

1. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

   Plasmid

   #133793

   Purpose

   U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   CjeCas9 sgRNA entry vector
   

   Expression

   Mammalian

   Mutation

   V2-TGAA linker sgRNA architecture from Kim et al.…

   Promoter

   U6

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-TAP(FLAG-HA) CYLD

   Plasmid

   #60031

   Purpose

   CYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintained

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   Cylindromatosis (CYLD Human)
   

   Use

   Retroviral

   Tags

   FLAG, HA, and IRES Puro

   Expression

   Mammalian

   Promoter

   LTR-driven expression

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pRSET-A2-77p-Venus

   Plasmid

   #64856

   Purpose

   Expresses aa 1594-1670 of A2 Domain of VWF protein, TEV cleavage site and Venus followed by 7X His tag

   Depositor

   Sriram Neelamegham

   Article

   Gogia et al PLoS One. 2015 May 18;10(5):e0126556. do

   Insert

   VWF A2 domain aa 1594-1670_TEV_Venus_7X His (VWF Synthetic, Human)
   

   Tags

   7X His and Venus

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCherry-3xNLS-OptoJNKi

   Plasmid

   #89740

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   NLS-OptoJNKi
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDF-casBCDE(g8)

   Plasmid

   #89727

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing 33-nt spacer (referred to as 'wt') matching a protospacer in the M13 phage genome.

   Depositor

   Konstantin Kuznedelov, Konstantin Severinov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. KB501: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; CMV promoter

   Plasmid

   #121813

   Purpose

   pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKCAG-mCherry-OptoJNKi

   Plasmid

   #89738

   Purpose

   Expression of light-regulated JNK inhibitor, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   OptoJNKi
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. CSK_HUMAN_D0

   Plasmid

   #79683

   Purpose

   This plasmid encodes the kinase domain of CSK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, Markus Seeliger, John Chodera

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   CSK (CSK Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pmCherry-NES-OptoJNKi

   Plasmid

   #89739

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   NLS-OptoJNKi
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKCAG-H2BmCherry-OptoJNKi

    Plasmid

    #89741

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    OptoJNKi
    

    Use

    Optogenetics

    Tags

    Histone2B and mCherry

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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