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We narrowed to 15,882 results for: LEA

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Showing: 7721 - 7740 of 15882 results

  1. AAVS1-eTLR

    Plasmid
    #214737
    Purpose
    encodes the enhanced traffic light reporter (eTLR) flanked by homology arms for integration into the AAVS1 safe harbor locus
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4
    Insert
    AAVS1-eTLR (PPP1R12C CMV, Anguilla japonica, influenza, Bovine, Human, Synthetic, Rabbit, Chicken)
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214737-plasmid-map-sequence-id-425005

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  3. pAAV-hSyn-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

    Plasmid
    #220610
    Purpose
    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-KORD-P2A-ArgiNLS-AausFP1
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    220610-plasmid-map-sequence-id-441133

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  4. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

    Plasmid
    #228253
    Purpose
    pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    U6
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    228253-plasmid-map-sequence-id-451024

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  7. pAAV-EF1a-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

    Plasmid
    #220613
    Purpose
    Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only

  8. pAAV-EF1a-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

    Plasmid
    #220614
    Purpose
    Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
    Use
    AAV and Cre/Lox
    Tags
    3x HA; ArgiNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    220614-plasmid-map-sequence-id-441114

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  9. pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3

    Plasmid
    #105802
    Purpose
    Concomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-mRuby3; ORF2: TEV-mClover3
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105802-plasmid-map-sequence-id-203396

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  11. pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF)

    Plasmid
    #182652
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & 4x M15 tRNACUA used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    4xU6-tRNAM15
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available Since
    May 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182652-plasmid-map-sequence-id-440860

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  12. pcDNA3.1-EF1A-mNeonGreen2x-SREBP1-P2A-Puro

    Plasmid
    #228401
    Purpose
    Encodes a N-terminal truncated version of SREBP1 lacking part of the transcriptional activation domain and fused to a tandem of mNeongreen to visualize the import of SREBP1
    Depositor
    Dirk Daelemans
    Article
    Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:
    Insert
    SREBP1 (SREBF1 Human)
    Tags
    mNeonGreen2x
    Expression
    Mammalian
    Mutation
    deleted N terminal activation domain (amino acids…
    Promoter
    EF1a
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    228401-plasmid-map-sequence-id-457257

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  15. pelB-MBP-HtrA-ss

    Plasmid
    #137066
    Purpose
    E. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature HtrA: lacks the HtrA signal peptide
    Promoter
    T7lac
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137066-plasmid-map-sequence-id-291956

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  19. ATX3-13Q

    Plasmid
    #184248
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Synthetic, Human)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    C-terminal codon optimization for protein expres…
    Promoter
    T7 promoter
    Available Since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    184248-plasmid-map-sequence-id-370322

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  20. pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP

    Plasmid
    #176279
    Purpose
    Viral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.
    Depositor
    Martin Riccomagno
    Article
    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    Kir2.1MUT-P2A-EGFP (Kcnj2 Synthetic, Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    GYG to AAA (aa144-146)
    Promoter
    human Synapsin I
    Available Since
    Jan. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    176279-plasmid-map-sequence-id-350684

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Showing: 7721 - 7740 of 15882 results