Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 12,028 results for: LEA

Clear keywords
Showing: 7721 - 7740 of 12028 results

  1. pMIG-hNFATc2/C(1-686)AVITEV

    Plasmid
    #74054
    Purpose
    retroviral expression plasmid for human NFATc2/C (without C-terminal region) with C-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc2, isoform C (NFATC2 AS 1-686 (N terminus deleted), Human)
    Use
    Retroviral
    Tags
    AVI-TEV
    Expression
    Mammalian
    Mutation
    silent mutation A1723C in the sequence of NM_1730…
    Promoter
    pMSCV-LTRs
    Available Since
    June 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    74054-plasmid-map-sequence-id-142271

    Enlarge

  2. pAAV/CMV-SCLM

    Plasmid
    #216758
    Purpose
    AAV2 transfer vector with the CMV promoter for ubiquitous expression of SuperClomeleon
    Depositor
    George Augustine
    Insert
    SuperClomeleon followed by WPRE and human growth hormone polyA terminator
    Use
    AAV
    Expression
    Mammalian
    Mutation
    S30R and 11 AA deletion in the Cerulean CFP moiet…
    Promoter
    CMV (cytomegalovirus)
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216758-plasmid-map-sequence-id-429489

    Enlarge

  3. pαH-S-GSAS-2P-N317P

    Plasmid
    #196378
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein with mutation N317P with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Article
    Manrique et al iScience. 2023 Jan 20;26(1):105855. doi:
    Insert
    S-GSAS-2P-N317P (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only

  4. pSN-A112C-1A32

    Plasmid
    #191241
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available Since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191241-plasmid-map-sequence-id-384667

    Enlarge

  5. pSN-A112C-1A31

    Plasmid
    #191242
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available Since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191242-plasmid-map-sequence-id-382360

    Enlarge

  6. pSN-A112C-CP8B1

    Plasmid
    #191240
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191240-plasmid-map-sequence-id-380472

    Enlarge

  7. pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS

    Plasmid
    #224697
    Purpose
    Bacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Gly-Gly-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    July 2, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224697-plasmid-map-sequence-id-444276

    Enlarge

  8. pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEV

    Plasmid
    #74055
    Purpose
    retroviral expression plasmid for human NFATc2/C(1-686, RIT) (without C-terminal region, with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc2, isoform C (NFATC2 AS 1-686 (N terminus deleted), Human)
    Use
    Retroviral
    Tags
    AVI-TEV and FLAG
    Expression
    Mammalian
    Mutation
    silent mutation A1723C in the sequence of NM_1730…
    Available Since
    June 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    74055-plasmid-map-sequence-id-142272

    Enlarge

  9. pET21b(+)_SARS-CoV-2_3CLpro_C145A-HIS

    Plasmid
    #224698
    Purpose
    Bacterial Expression and Purification of SARS-CoV-2 3CLpro inactive protease with His-tag
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Gly-Gly-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    July 2, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224698-plasmid-map-sequence-id-444264

    Enlarge

  10. pcDNA4/TO-bio-myc-cCE

    Plasmid
    #112711
    Purpose
    For tetracycline-inducible expression of bio-myc-NES-mCE ΔNLS ("bio-myc-cCE") in mammalian cells
    Depositor
    Daniel Schoenberg
    Article
    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1
    Insert
    Rngtt (Rngtt Mouse)
    Tags
    HIV Rev nuclear localization signal (NES), Stop c…
    Expression
    Mammalian
    Mutation
    Silent mutation at Arg 132 (CGT to CGC), deleted …
    Promoter
    CMV with Tet repressor binding sites
    Available Since
    Sept. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    112711-plasmid-map-sequence-id-217051

    Enlarge

  11. C13m

    Plasmid
    #120960
    Purpose
    MoClo golden gate assembly CD part for Cerulean (modified CFP; variant of https://www.fpbase.org/protein/cerulean/) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Cerulean
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120960-plasmid-map-sequence-id-245422

    Enlarge

  16. pN_empty

    Plasmid
    #140404
    Purpose
    Empty backbone with nourseothricin selection in plants
    Depositor
    Mathias Pribil
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    140404-plasmid-map-sequence-id-292691

    Enlarge

  18. C95m

    Plasmid
    #121001
    Purpose
    MoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    T7 RNA polymerase (Andy please check!)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121001-plasmid-map-sequence-id-236130

    Enlarge

Showing: 7721 - 7740 of 12028 results