We narrowed to 15,732 results for: LEA

1. pBactin-Akna-mEGFP

   Plasmid

   #196866

   Purpose

   Expression of the centrosomal protein Akna fused to enhanced (E)-GFP

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akna-mEGFP (Akna Mouse)
   

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBactin-td-mCherry-Cdk5rap2

   Plasmid

   #196859

   Purpose

   Expression of Cyclin dependent kinase 5 regulatory subunit-associated protein 2 (Cdk5rap2) fused to tandem (td)-mCherry.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   td-mCherry-Cdk5rap2 (Cdk5rap2 Rat)
   

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Control click editor - pCMV-T7-PCV2-nCas9-deadEcKlenow (JO520)

   Plasmid

   #208945

   Purpose

   A control CE1 construct with catalytically attenuated EcKlenow DNA polymerase, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-dEcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A);deadEcKlenow(-exo;D355A,D357A,D705…

   Promoter

   CMV and T7

   Available Since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. ISG15 C-term. Domain intein/chitin binding domain (79-156)

   Plasmid

   #110760

   Purpose

   Bacterial expression of ISG15 C-term.

   Depositor

   David Komander

   Article

   Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

   Insert

   ISG15 (ISG15 Human)
   

   Tags

   intein/chitin binding domain

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4 h-Stx3 resistant to shRNA #304-2xMyc/His

   Plasmid

   #99746

   Purpose

   Expresses human Stx3 with a C-term myc-myc-his that is resistant to shRNA #304 from Sigma Aldrich.

   Depositor

   Thomas Weimbs

   Article

   Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

   Insert

   Stx3 (STX3 Human)
   

   Tags

   myc-myc-his

   Expression

   Mammalian

   Mutation

   Made six (6) silent mutations to confer resistanc…

   Promoter

   CMV/TO

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo

   Plasmid

   #112684

   Purpose

   An AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinase

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   iRFP713
   

   Use

   AAV

   Tags

   2A-Flpo and Nuclear localization signal

   Promoter

   CMV-IE

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBridge-tyrosinase.σ1A

   Plasmid

   #197381

   Purpose

   Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Inserts

   tyrosinase cytosolic tail
   AP-1 σ1A
   

   Tags

   GAL4-DNA binding domain fragment, HA tag, and SV4…

   Expression

   Yeast

   Mutation

   Contains an extra 42 nucleotides encoding 14 resi…

   Promoter

   ADH1 and MET25

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-HA-Nr4a1-AA

   Plasmid

   #160942

   Purpose

   Generation of retrovirus for the overexpression of Nr4a1 mutant (287C 288EAA)

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Nr4a1 (Nr4a1 Mouse)
   

   Use

   Retroviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Change Cys 287 Glu 288 into Ala Ala

   Promoter

   MSCV-LTR

   Available Since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pelB-MBP-RevA-ss

   Plasmid

   #137064

   Purpose

   E. coli expression clone (T7lac promoter) for mature RevA (aa 25-160) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAF07416.1 (revA Borrelia burgdorferi B31)
   

   Tags

   PelB signal peptide + His10 + maltose binding pro…

   Expression

   Bacterial

   Mutation

   lacks the RevA signal peptide and 5 residues (CKA…

   Promoter

   T7lac

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pelB-MBP-DipA-ss

    Plasmid

    #137068

    Purpose

    E. coli expression clone (T7lac promoter) for mature DipA (aa 21-342) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
    

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature DipA: lacks the DipA signal peptide

    Promoter

    T7lac

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-NLS-DNMT3A-L-N-spC9-N-intein-hGH

    Plasmid

    #112212

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    N-terminus of dCas9 and DNMT3A (DNMT3A Human)
    

    Use

    AAV

    Mutation

    H840A,N863A

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFUSEss-CHIg-mM-Asn209Ala_M18

    Plasmid

    #91733

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ala (EU numbering).

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Expression

    Mammalian

    Mutation

    Asn209 changed to Ala (EU numbering)

    Promoter

    hEF1-HTLV prom

    Available Since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOTTC710 - pAAV EF1a DIO Mem-AcGFP

    Plasmid

    #75081

    Purpose

    An AAV packaging vector that expresses Cre-dependent membrane-localized AcGFP under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

    Insert

    Mem-AcGFP
    

    Use

    AAV and Cre/Lox

    Tags

    Palmitylation site

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX459-dCTNNA1

    Plasmid

    #176032

    Purpose

    A knock-out vector for dog CTNNA1 (alpha-1-catenin)

    Depositor

    Michiyuki Matsuda

    Article

    Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

    Insert

    A gRNA targeting the dog CTNNA1 (alpha-1-catenin) gene and the cDNA of Cas9 (CTNNA1 )
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-hAR-R617M,K618M,K632M,K633M

    Plasmid

    #115778

    Purpose

    Expresses human androgen receptor (R617M, K618M, K632M, K633M) in mammalian cells

    Depositor

    Elizabeth Wilson

    Article

    Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

    Insert

    Androgen Receptor (AR Human)
    

    Expression

    Mammalian

    Mutation

    R617M, K618M ,K632M, K633M, insertion of Q81 and …

    Promoter

    CMV

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3-Flag-SPRTN-Y117C

    Plasmid

    #110216

    Purpose

    Expression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.

    Depositor

    Ivana Marinovic-Terzic, Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Y117C, P296L (see depositor comments below)

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-NLS-YFP-CCTd

    Plasmid

    #184325

    Purpose

    Mammalian expression of the nuclear localized YFP-CCTd(peptide of Cav1.3)

    Depositor

    Xiaodong Liu

    Article

    Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

    Insert

    CCTd (CACNA1D Human)
    

    Expression

    Mammalian

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1/TO-myc-BirA*-cCE

    Plasmid

    #112713

    Purpose

    For tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

    Insert

    Rngtt (Rngtt Mouse)
    

    Tags

    BirA* (BirA R118G), HIV Rev nuclear localization …

    Expression

    Mammalian

    Mutation

    Silent mutation at Arg 132 (CGT to CGC), deleted …

    Promoter

    CMV

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. ISG15 C-term. Domain deltaC intein/chitin binding domain (79-154)

    Plasmid

    #110761

    Purpose

    Bacterial expression of proISG15

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    ISG15 (ISG15 Human)
    

    Tags

    intein/chitin binding domain

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75244

    Purpose

    CRISPR/Cas9 plasmid against human Helios

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Helios (IKZF2 Human)
    

    Use

    CRISPR

    Available Since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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