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We narrowed to 20,285 results for: ACE;

Showing: 11611 - 11640 of 20285 results

  2. pNICKclos1.0

    Plasmid
    #73639
    Purpose
    Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824
    Depositor
    Sheng Yang
    Article
    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/
    Inserts
    Cas9 nickase
    sGRNA to pyrE
    Use
    E.coli-clostridium shuttle vector
    Tags
    Expression
    Mutation
    D10A
    Promoter
    j23119 and ptb
    Available Since
    June 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    73639-plasmid-map-sequence-id-148603

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  4. mbYFPQS

    Plasmid
    #80742
    Purpose
    membrane-targeted biosensor for monitoring changes in intracellular chloride
    Depositor
    Susan Ingram, Susan Amara
    Article
    Watts et al PLoS One. 2012;7(4):e35373. doi: 10.1371
    Insert
    YFPQS
    Use
    Tags
    palmitoylation signal sequence of neuromodulin
    Expression
    Mammalian
    Mutation
    H148Q (CAC to CAG) and V163S (GTG to AGC).
    Promoter
    CMV
    Available Since
    Aug. 3, 2016
    Availability
    Academic Institutions and Nonprofits only

  5. pdCASclos

    Plasmid
    #73640
    Purpose
    Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824
    Depositor
    Sheng Yang
    Article
    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/
    Inserts
    dCas9
    sgRNA to spo0A
    Use
    E.coli-clostridium shuttle vector
    Tags
    Expression
    Mutation
    D10A, H840A
    Promoter
    Pj23119 and ptb
    Available Since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73640-plasmid-map-sequence-id-148604

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  9. His-NavMs

    Plasmid
    #100004
    Purpose
    Electrophysiology in mammalian cells: Mammalian expression of voltage gated sodium channel and GFP
    Depositor
    Paul DeCaen
    Article
    Sula et al Nat Commun. 2017 Feb 16;8:14205. doi: 10
    Insert
    His-NavMs, Voltage gated sodium channel
    Use
    Tags
    Hexa-His tag followed by a thrombin cleavage site
    Expression
    Mammalian
    Mutation
    C-terminus from a codon-optimized synthetic gene,…
    Promoter
    CMV
    Available Since
    Aug. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100004-plasmid-map-sequence-id-192452

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  14. pT7-AsCas12a_crRNA-site4 (MSP3511)

    Plasmid
    #160139
    Purpose
    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Insert
    AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
    Use
    In vitro transcription of sgrna from t7 promoter
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available Since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    160139-plasmid-map-sequence-id-333510

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  16. pCH6 - pBait-ChiX

    Plasmid
    #174662
    Purpose
    arabinose-induced synthesis of a hybrid RNA containing a single MS2 hairpin followed by E coli ChiX inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.
    Depositor
    Katherine Berry
    Article
    Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507
    Insert
    ChiX
    Use
    Tags
    1xMS2 hairpin
    Expression
    Bacterial
    Mutation
    Promoter
    pBad
    Available Since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    174662-plasmid-map-sequence-id-361921

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  18. shNTC

    Plasmid
    #73549
    Purpose
    Non-targeting control shRNA cloned in retroviral vector pMLP (MSCV-based vector expressing shRNA in a mir30 context).
    Depositor
    Gerardo Ferbeyre
    Article
    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d
    Insert
    shNTC
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    LTR
    Available Since
    Aug. 8, 2016
    Availability
    Academic Institutions and Nonprofits only

  27. pONSY

    Plasmid
    #111873
    Purpose
    "Capsaspora owczarzaki" expression vector (backbone) modified from the pCR2.1. It bears the promoter and terminator regions from the endogenous Elongation Factor 1 alpha (EF1a) gene (CAOG_07807).
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Type
    Empty backbone
    Use
    Capsaspora owczarzaki
    Tags
    Expression
    Mutation
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available Since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    111873-plasmid-map-sequence-id-217304

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  28. pWL502-PAM

    Plasmid
    #174382
    Purpose
    Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei
    Depositor
    Jing Han
    Article
    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:
    Insert
    PAM-spacer
    Use
    CRISPR; Archaeal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    174382-plasmid-map-sequence-id-344136

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  29. pT7-AsCas12a_crRNA-site3 (RTW549)

    Plasmid
    #160138
    Purpose
    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Insert
    AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)
    Use
    In vitro transcription of sgrna from t7 promoter
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available Since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    160138-plasmid-map-sequence-id-333508

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Showing: 11611 - 11640 of 20285 results