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Plasmid#207064PurposeExpressing GFP-Pex3-4V5-APEX2 under ADH3 promoter. Insufficient signal for EM.DepositorInsertPex3
UseTags4xV5-APEX2 and GFPExpressionYeastMutationPromoterpADH3Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-OM14-V5-APEX2-Ura
Plasmid#207073PurposeExpressing OM14-V5-APEX2. Marker for mitochondrial outer membrane.DepositorInsertOM14
UseTagsV5-APEX2ExpressionYeastMutationPromoterpOM14Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-Cox4-V5-APEX2-Ura
Plasmid#207061PurposeExpressing Cox4-V5-APEX2. Marker for mitochondrial inner membrane. Tends to generate facture in thin resin sections.DepositorInsertCox4
UseTagsV5-APEX2ExpressionYeastMutationPromoterpCOX4Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-Cox4-GFP-APEX2-Ura
Plasmid#207062PurposeExpressing Cox4-GFP-APEX2. GFP interferes with APEX staining.DepositorInsertCox4
UseTagsGFP-APEX2ExpressionYeastMutationPromoterpCOX4Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pADH3-Snf7-4V5-APEX2-URA
Plasmid#207065PurposeModerate overexpression of Snf7-4V5-APEX2 under ADH3 promoter. Marker for late endosomes. Only partially functional.DepositorInsertSnf7
UseTags4xV5-APEX2ExpressionYeastMutationPromoterpADH3Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pERG6-Pex3-4V5-APEX2-URA
Plasmid#207068PurposeModerate overexpression of Pex3-4V5-APEX2 under ERG6 promoter. Marker for peroxisomes.DepositorInsertPex3
UseTags4xV5-APEX2ExpressionYeastMutationPromoterpERG6Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pLSP1-Pex3-4V5-APEX2-URA
Plasmid#207069PurposeModerate overexpression of Pex3-4V5-APEX2 under LSP1 promoter. Marker for peroxisomes.DepositorInsertPex3
UseTags4xV5-APEX2ExpressionYeastMutationPromoterpLSP1Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pTPI1-Emc1-GFP-APEX2-Ura
Plasmid#207074PurposeExpressing Emc1-GFP-APEX2. GFP interferes with APEX staining.DepositorInsertEmc1
UseTagsGFP-APEX2ExpressionYeastMutationPromoterpTPI1Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pTPI1-Cox4-GFP-APEX2-Ura
Plasmid#207075PurposeExpressing Cox4-GFP-APEX2. GFP interferes with APEX staining.DepositorInsertCox4
UseTagsGFP-APEX2ExpressionYeastMutationPromoterpTPI1Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-RfA-HA
Plasmid#186374PurposeDestination vector for the expression of a fusion of an N-ter protein of interest with an HA tag into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-HA-RfA
Plasmid#186373PurposeDestination vector for the expression of a fusion of N-ter HA tag and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-RfA-Venus
Plasmid#186372PurposeDestination vector for the expression of a fusion of an N-ter protein of interest with Venus into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-Venus-RfA
Plasmid#186371PurposeDestination vector for the expression of a fluorescent fusion of N-ter Venus and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-fCLIP-GS
Plasmid#188939PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-fCLIP-GS
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-fSNAP-GS
Plasmid#188938PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-fSNAP-GS
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-DHFR-3xHA
Plasmid#188934PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-DHFR-3xHA
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCK26
Plasmid#105429PurposeSynthetic biologyDepositorInsertBs3
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGM16841
Plasmid#105416PurposeSynthetic biologyDepositorInsertSTOP codon and nos terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG131
Plasmid#105337PurposeGateway entry clone generationDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG267
Plasmid#105339PurposeSynthetic biology; gateway destination vector assemblyDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only