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Viral Prep#92385-LVPurposeReady-to-use Lentiviral Prep particles produced from Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set A (#92385). In addition to the viral particles, you will also receive purified Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set A plasmid DNA.DepositorPromoterTagsNoneAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only
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Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (Lentiviral Prep)
Viral Prep#92379-LVPurposeReady-to-use Lentiviral Prep particles produced from Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (#92379). In addition to the viral particles, you will also receive purified Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR activation screening in human cells. Contains 56,762 sgRNAs, targeting 18,885 genes. Concentrated lentiviral particles carrying set A of the Calabrese human CRISPRa sgRNA activation library in backbone XPR_502 (P65 HSF) .DepositorPromoterTagsNoneAvailable sinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
SETD2(SET)-Cas9
Plasmid#186700PurposePlasmid encoding SETD2-Cas9 fusion under CMV promoterDepositorInsertSETD2(SET)-Cas9
UseCRISPRTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-SET-EMD (N-SET-EMD)
Plasmid#217765PurposeFluorescent reporter for ceramide (putative, mammalian expression)DepositorInsertSET (SET Human)
UseTagsEGFPExpressionMammalianMutationEMD domain (aa 70-226)PromoterCMVAvailable sinceJune 12, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSET-EMD-EGFP (C-SET-EMD)
Plasmid#217764PurposeFluorescent reporter for ceramide (putative, mammalian expression)DepositorInsertSET (SET Human)
UseTagsEGFPExpressionMammalianMutationEMD domain (aa 70-226)PromoterCMVAvailable sinceJune 12, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNES-SET-EMD-EGFP (C-NES-SET-EMD)
Plasmid#217770PurposeFluorescent reporter for ceramide (putative, mammalian expression)DepositorInsertSET (SET Human)
UseTagsEGFPExpressionMammalianMutationEMD domain (aa 70-226) plus nuclear export sequen…PromoterCMVAvailable sinceJune 12, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-SET
Plasmid#234708Purposeexpresses His-tagged human SET in E coliDepositorInsertSET (SET Human)
UseTags(His)6ExpressionBacterialMutationPromoterlacIAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET26b-SET
Plasmid#8822DepositorInsertSET (SET Human)
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_IGLL1_C1-set
Plasmid#109991PurposeProtein expression and purification of IGLL1_C1-setDepositorInsertIGLL1_C1-set (IGLL1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
SETDB1
Plasmid#25240PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSETDB1, SETDB1_16 (SETDB1 Human)
UseTags6xHis-TEV and MHHHHHHSSGRENLYFQGExpressionBacterialMutationPromoterT7Available sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRosetta
Plasmid#59700PurposeLentiviral vector for use as a standard for titering experiments when trying to compare vectors with different selection markers or to determine appropriate antibiotic conditions.DepositorInsertsPuro
BlastR
EGFP
UseLentiviralTags2AExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRosetta_v2
Plasmid#136477PurposeLentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycinDepositorInsertHygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SETDB1_WT
Plasmid#82291PurposeGateway Donor vector containing SETDB1, part of the Target Accelerator Plasmid Collection.DepositorInsertSETDB1 (SETDB1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
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CeNL_pRSETb
Plasmid#89510PurposeCyan color variant of bright luminescent protein for bacterial expressionDepositorInsertCeNL
UseTagsHis X 6ExpressionBacterialMutationPromoterT7Available sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
ReNL_pRSETb
Plasmid#89536PurposeRed color variant of bright luminescent protein for bacterial expressionDepositorInsertReNL
UseTagsHis X 6ExpressionBacterialMutationPromoterT7Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRosettav2
Plasmid#115557PurposeTo determine correct antibiotic selection conditions in mammalian cells; standard for titering experimentsDepositorInsertHygromycin-Blasticidin-Puromycin-EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_crGE
Plasmid#172927PurposeCrowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.DepositorInsertCrowdingSensorGE
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
YeNL_pRSETb
Plasmid#89520PurposeYellow color variant of bright luminescent protein for bacterial expressionDepositorInsertYeNL
UseTagsHis X 6ExpressionBacterialMutationPromoterT7Available sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SETD8_WT
Plasmid#82290PurposeGateway Donor vector containing SETD8, part of the Target Accelerator Plasmid Collection.DepositorInsertSETD8 (KMT5A Human)
UseGateway entry vectorTagsExpressionMutationP316RPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only