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Plasmid#104991PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only
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lenti-SpCas9 hygro
Plasmid#104995PurposeThis lentiviral construct delivers hSpCas9 and hygromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 neo
Plasmid#104996PurposeThis lentiviral construct delivers hSpCas9 and G418 resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 blast
Plasmid#104997PurposeThis lentiviral construct delivers hSpCas9 and blasticidin S resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pF CAG luc IRES neo
Plasmid#98294PurposeFor constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance geneDepositorInsertsFirefly luciferase
IRES
Neomycin resistance gene (NeoR)
UseLentiviral and LuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA neo
Plasmid#104992PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJF3H
Plasmid#63897PurposeFor phage display of cDNA librariesDepositorInsertcDNA cloning region
UsePhage displayTagsFosExpressionBacterialMutationPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHelper_TS_V2_ampR
Plasmid#214467PurposeHelper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Includes temperature sensitive origin for plasmid curing.DepositorArticleInsertCspRecT / Bxb-1
UseSynthetic BiologyTagsExpressionMutationPromoterXylS / AraCAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMR150
Plasmid#32292DepositorInsertgenomic methylation probe-minor satellite repeat
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBSG01
Plasmid#37558DepositorInsertPreScission protease cleavage site-S tag-TEV site-Z
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL3_MSR1_bothneg0topos3pos1
Plasmid#232387PurposeMSR1 enhancer, left buffering Coordinator converted to sensitizing double Ebox and right buffering Coordinator to sensitizing CoordinatorDepositorInsertMSR1 (MSR1 Human)
UseLuciferaseTagsExpressionMutationMSR1 enhancer, left buffering Coordinator convert…PromoterAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSEVA83-GG
Plasmid#208016Purposefor cloning homology arms, with GFP as counter selection markerDepositorInsertmsfGFP
UseTagsExpressionMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTrc-CysGA-Im7 WT
Plasmid#201081PurposeIm7 WT inserted into CysGA at G364 via GS linkerDepositorInsertCysGA-Im7
UseTagsExpressionBacterialMutationPromoterpTrcAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTrc-CysGA-Linker only
Plasmid#201080PurposeGS linker inserted into CysGA at G364DepositorInsertCysGA-Linker only
UseTagsExpressionBacterialMutationPromoterpTrcAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEVA13-GG
Plasmid#208002Purposefor cloning homology arms, with GFP as counter selection markerDepositorInsertmsfGFP
UseTagsExpressionMutationPromoter14GAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEVA43-GG
Plasmid#208014Purposefor cloning homology arms, with GFP as counter selection markerDepositorInsertmsfGFP
UseTagsExpressionMutationPromoter14GAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEVA63-GG
Plasmid#208015Purposefor cloning homology arms, with GFP as counter selection markerDepositorInsertmsfGFP
UseTagsExpressionMutationPromoter14GAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEVA23-GG
Plasmid#208013Purposefor cloning homology arms, with GFP as counter selection markerDepositorInsertmsfGFP
UseTagsExpressionMutationPromoter14GAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
YN2_1_LT58_X2site
Plasmid#177705PurposeExpresses Cas9 and sgRNA cassette for targeting site X2 in yeast Saccharomyces cerevisiaeDepositorInsertCas9
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
LT1_36_QUEEN-2m
Plasmid#177708PurposePlasmid for genome integration in X2 site expressing QUEEN-2m (ATP biosensor)DepositorInsertpTEF1-QUEEN2m
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only