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pSicoR-SLC17A5i2

Plasmid

#59599

Purpose

Expression of shRNA against human SLC17A5

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

SLC17A5 (SLC17A5 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-UBE2D3i2

Plasmid

#59589

Purpose

Expression of shRNA against human UBE2D3

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

UBE2D3 (UBE2D3 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-myc-Rem2 S69A/S241A/S308A/S334A

Plasmid

#51594

Purpose

expresses RNAiR myc-tagged Rem2 S-A mutant

Depositor

Suzanne Paradis

Article

Ghiretti et al J Neurosci. 2013 Apr 10;33(15):6504-15.

Insert

Rem2 (Rem2 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Serines 69, 241, 308, 334 to Alanines; also RNAi …

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
GST-30A&B

Plasmid

#1847

Depositor

Philip Leder

Article

Bedford et al J Biol Chem 2000 Apr 7;275(14):10359-69.

Insert

FBP30 WW-A WW-B (Fnbp4 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA2

Plasmid

#133341

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the template strand in Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_gcrA

Use

CRISPR

Tags

Expression

Mutation

Promoter

constitutive

Available since

Availability

Academic Institutions and Nonprofits only

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pLX401-INK4A

Plasmid

#121919

Purpose

Doxycycline-inducible expression of p16-INK4A product of CDKN2A

Depositor

William Hahn

Article

Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

Insert

p16-INK4A (CDKN2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE Tet ON

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCdrA-gfpC

Plasmid

#111614

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(Mut3)-T0-T1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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M50 Super 8x TOPFlash

Plasmid

#12456

Purpose

Beta-catenin reporter. TCF/LEF sites upstream of a luciferase reporter.

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

TCF/LEF binding sites (Ctnnb1 )

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 13, 2007

Availability

Academic Institutions and Nonprofits only

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STAT3-GFP

Plasmid

#110495

Purpose

reporter of STAT3 signaling activity

Depositor

Michael Lewis

Article

Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

Insert

4 repeats of STAT3 binding site M67 at GFP promoter region

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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MT1E

Plasmid

#105702

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 1e

Depositor

Artur Krężel

Article

Peris-Diaz et al J Proteome Res. 2021 Jan 1;20(1):776-785

Insert

Metallothionein 1e (MT1E Human)

Use

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pHRSin_1G4 TCRα

Plasmid

#187572

Purpose

Lentiviral expression TCR alpha(1G4)

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

1G4 TCR alpha (TRA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMK290 (mAID-mClover-Hygro)

Plasmid

#72828

Purpose

C-terminal tagging with mAID-mClover using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mClover-Hygro

Use

CRISPR

Tags

mAID-mClover

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Smad2-3'UTR

Plasmid

#61775

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad2

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad2 3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Smad5-3'UTR

Plasmid

#61777

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad5

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad5 3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
MSGV Mouse Acceptor PGK NGFR

Plasmid

#128482

Purpose

A TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR expressing NGFR ref Biotechniques. 2015 Mar 1;58(3):135-9

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

MSCV

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMK289 (mAID-mClover-NeoR)

Plasmid

#72827

Purpose

C-terminal tagging with mAID-mClover using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mClover-Neo

Use

CRISPR

Tags

mAID-mClover

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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sgLenti

Plasmid

#105996

Purpose

single sgRNA expression vector

Depositor

Mike McManus

Article

Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

Type

Empty backbone

Use

CRISPR and Lentiviral; S.pyogenes sgrna expressio…

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMK286 (mAID-NeoR)

Plasmid

#72824

Purpose

C-terminal tagging with mAID using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-Neo

Use

CRISPR

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMK292 (mAID-mCherry2-NeoR)

Plasmid

#72830

Purpose

C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mCherry2-Neo

Use

CRISPR

Tags

mAID-mCherry2

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMM0646

Plasmid

#129007

Purpose

Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

KanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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