We narrowed to 17,163 results for: EMB

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pCI5-CasRx-NLS-HA (pKW919)

Plasmid

#229047

Purpose

Mammalian expression of CasRx-NLS-HA

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Insert

CasRx (RfxCas13d)

Use

CRISPR

Tags

NLS and NLS-HA

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PM-deltaCKAR

Plasmid

#222429

Purpose

FRET-based reporter for monitoring deltaPKC activity at the plasma membrane in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Plasma membrane delta-selective C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pRSET_SynPspA

Plasmid

#223812

Purpose

Bacterial expression of SynPspA with N-terminal 10xHis-tag and EK cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Cell. 2021 Jul 8;184(14):3674-3688.e18.

Insert

SynPspA

Tags

10xHis and NusA

Expression

Bacterial

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-PKP3(305-797)

Plasmid

#202599

Purpose

Expresses ARM domain of plakophilin3

Depositor

Tina Izard

Article

Gupta et al Int J Mol Sci. 2023 May 29;24(11):9458.

Insert

plakophilin3 (PKP3 Human)

Tags

His8 tag with precision protease cleavage site

Expression

Bacterial

Mutation

Deleted amino acids 1 to 304

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAG66

Plasmid

#226751

Purpose

Expresses exogenous GFP

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

GFP

Use

Lentiviral

Tags

sfGFP

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJB53

Plasmid

#226309

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with V(T/S)G to AAA CsoS2 MR1-3

Expression

Bacterial

Mutation

VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

Promoter

pTRC

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLPP4

Plasmid

#209966

Purpose

Contains Level 0 Part: constitutive Promoter (P40) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P40)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLP-EV

Plasmid

#209973

Purpose

Contains Level 0 Part: mock coding sequence (EV) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

CDS (mock)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPC53

Plasmid

#209962

Purpose

Contains Level 0 Part: 5' Connector (5C1CSN with double terminator Tcat+sai) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

5' Connector (5C1CSN_Tcat+sai)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCI-SMN2-inclusion-GLuc

Plasmid

#218671

Purpose

Alternative splicing signaling: signal increased with Ex7 inclusion increasing

Depositor

Jingxin Wang

Article

Tang et al Chembiochem. 2022 May 4;23(9):e202200012

Insert

SMN2 (SMN2 )

Expression

Mammalian

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyA-GFP

Plasmid

#220189

Purpose

sgRNA expression vector - pEM7 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pEM7 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pEM7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyD-GFP

Plasmid

#220192

Purpose

sgRNA expression vector - pBG28 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG28 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG28

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRS-GFP

Plasmid

#220197

Purpose

mini-Tn7 GFP expression vector

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

promoterless

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMK468 (BSD-P2A-mAID-dTAG)

Plasmid

#214394

Purpose

mAID-dTAG for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BSD-P2A-mAID-dTAG

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK452 (BSD-P2A-dTAG-mClover)

Plasmid

#214382

Purpose

dTAG-mClover for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BSD-P2A-dTAG-mClover

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-IvfChr-citrine

Plasmid

#221614

Purpose

A recombinant AAV2 plasmid encoding vfChrimson-citrine with trafficking enhancement.

Depositor

John Lin

Article

Mermet-Joret et al eLife 2023 12:RP90327

Insert

vfChrimson-citrine with membrane trafficking enhancement

Use

AAV

Mutation

vfChrimson with membrane trafficking enhancement

Promoter

Human Synapsin

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-39S Light chain

Plasmid

#216297

Purpose

Expresses anti-HER2 39S Light chain in mammalian cells. To be paired with 39S heavy chain to form the anti-HER2 39S Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

39S Light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MF3958 Light chain

Plasmid

#216304

Purpose

Expresses anti-HER2 MF3958 Light chain in mammalian cells. To be paired with MF3958 heavy chain to form the anti-HER2 MF3958 Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

MF3958 Light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SnoopTag-SpyTag-(AffiHER2)x3

Plasmid

#216281

Purpose

Three affibodies to HER2 linked in a chain for bacterial expression. SnoopTag and SpyTag allow isopeptide bond formation to their cognate Catcher

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

SnoopTag-SpyTag-(AffiHER2)x3

Use

Affinity Reagent/ Antibody

Tags

His6, SnoopTag, and SpyTag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTP489

Plasmid

#216366

Purpose

xNup155-Nb1t (+3Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup155-Nb1t (+3Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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