We narrowed to 10,134 results for: ada
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Plasmid#51436PurposeThis plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.2 enhancerDepositorUseLuciferaseExpressionMammalianMutationThe ME.2 enhancer is fused upstream of the ApoE g…Available SinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only
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AP1muA-Halo-ALFA-polyA-G418 HR
Plasmid#229681PurposeHomology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.DepositorAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
loxP-G418-loxP-3xALFA-Halo-Rab11 HR
Plasmid#229676PurposeHomology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cellsDepositorAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-puro-NLS-mCherry
Plasmid#185474PurposeMammalian Expression, Lentiviral vector expressing mCherry for two-colour competitive growth assay. Clone guide oligos into BsmBI site.DepositorTypeEmpty backboneUseLentiviralTagsmCherryExpressionMammalianPromoterCMVAvailable SinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-EGFP-NLS-FLAG- cGAS E225A/D227A
Plasmid#127660PurposeOver-express catalytically inactive EGFP-NLS-FLAG-cGAS E225A/D227ADepositorAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV2-ITR-pEFS-NLS_hfCas13d(RfxCas13d_N2V8)_FLAG_NLS-pA-U6-DR-Pcsk9-sg5-DR-Pcsk9-sg6-DR-AAV2-ITR
Plasmid#191795Purposevector for encoding and AAV packaging a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by EFS promoter, Pcsk9-target guide RNAs compatible with RfxCas13d driven by hU6DepositorInserthumanized hfCas13d
UseAAV and CRISPRExpressionMammalianMutationA134V, A140V, A141V, A143VPromoterEFS, hU6Available SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
loxP-Puro-loxP-3xV5-SNAP-Rab11 HR
Plasmid#229677PurposeHomology repair plasmid for endogenous tagging of Rab11 at the N-terminus with SNAP tag and a 3x V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.DepositorAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-PARP1-E988K
Plasmid#211580PurposeCMV driven PARP1-E988K -eGFP expressing construct with IRES-Neomycin selectionDepositorAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-RUNX1
Plasmid#192928PurposeBarcoded piggybac transposon vector with Dox-inducible expression of RUNX1DepositorAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA
Plasmid#191797Purposevector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoterDepositorInserthumanized dCas13d
UseCRISPRExpressionMammalianMutationR239A, H244A, R858A, H863APromoterCBh, SV40, hU6Available SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-hMLH1dn
Plasmid#174827PurposeMammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)DepositorInsertPE2-P2A-hMLH1dn
TagsSV40 bpNLSExpressionMammalianMutationDetailed in manuscript; deletion of residues 754-…PromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-puro-NLS-GFP
Plasmid#185473PurposeMammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.DepositorTypeEmpty backboneUseLentiviralTagsGFPExpressionMammalianPromoterCMVAvailable SinceJuly 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPB-cT3G-cERP2-GATA4
Plasmid#192896PurposeBarcoded piggybac transposon vector with Dox-inducible expression of GATA4DepositorInsertGATA4 (GATA4 Human)
TagsHAExpressionMammalianMutationCodon-optimizedPromoterTET3G-miniCMVAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eGFP-uTEV3
Plasmid#234320PurposeMammalian expression of uTEV3 tagged with an eGFP markerDepositorInserteGFP-uTEV3
UseAAVTagseGFPExpressionMammalianMutationMutations respective to the wild type TEV: S219V …PromoterCMVAvailable SinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA
Plasmid#191796Purposevector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoterDepositorInserthumanized dCas13X
UseCRISPRExpressionMammalianMutationR84A, H89A, R739A, R740A, H745A, H746A, H747APromoterCBh, SV40, hU6Available SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2
Plasmid#217635PurposeAAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.DepositorInsertmU6-sgRNA, Handle sequence, EF1a-mTagBFP2
UseAAVPromoterEF1alpha and mU6Available SinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHDR-FOXL2-T2A-tdTomato-PuroTK
Plasmid#192892PurposeHDR donor for inserting FOXL2-T2A-tdTomato reporterDepositorInsertFOXL2-tdTomato (FOXL2 Human)
UseCRISPRMutationT2A-tdTomato fluorescent reporterPromoterPGKAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-GW
Plasmid#60323PurposeThis is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).DepositorTypeEmpty backboneUseLuciferaseTagsLuciferaseExpressionMammalianAvailable SinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-ARID1A-deltaIDR1-FLAG
Plasmid#242210PurposeExpression of human ARID1A ∆IDR1 (deletion of a.a. 2–970) using piggyBac transpositionDepositorInsertARID1A (ARID1A Human)
UsePiggybacTagsFLAGExpressionMammalianMutationDeleted IDR1 from ARID1A (deletion of a.a. 2–970)PromoterCAGAvailable SinceSept. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
PB-PARP1-del.p119K120S–eGFP
Plasmid#211554PurposeA piggyBac vector containing CMV-PARP1-del.p119K120S-eGFP-IRES-Neo cassette.DepositorInsertPARP1 (PARP1 Human)
UsePiggybacTagseGFPExpressionMammalianMutationdeletion of amino acids 119K 120SPromoterCMVAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only