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pTO136_pADH1-tRNA-Ala-gRNA-tracrRNA-HDV-tAgTEF2

Plasmid

#171104

Purpose

Expresses sgRNA (ENO1 gRNA) in Candida auris.

Depositor

Teresa O'Meara

Article

Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

Insert

sgRNA

Expression

Yeast

Promoter

ADH1

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRS414-ATG41

Plasmid

#113080

Purpose

Contain 1,000 nucleotides of the ATG41 5'UTR, the ATG41 open reading frame and 500 nucleotides of the 3' UTR.

Depositor

Daniel Klionsky

Article

Yao et al Autophagy. 2015;11(12):2288-99. doi: 10.

Insert

ATG41

Expression

Bacterial and Yeast

Promoter

ATG41 endogenous promoter

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYEXNHG3

Plasmid

#49025

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pSFS2A-CaHygB

Plasmid

#189564

Purpose

CaHygB hygromycin resistance gene in the pSFS2A flipper backbone for genetic manipulation of Candida albicans

Depositor

Richard Bennett

Article

Park et al mSphere. 2022 Aug 15:e0034722. doi: 10.1

Insert

HygB

Expression

Yeast

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pRS415-GPD

Plasmid

#183940

Purpose

Control vector that has a GPD promoter with no insert

Depositor

Michael Downey

Article

McCarthy et al mBio. 2022 Aug 30;13(4):e0039022. doi: 1

Type

Empty backbone

Expression

Yeast

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pRS424GFP-2xPH(Osh2)

Plasmid

#36095

Depositor

Scott Emr

Article

Stefan et al Cell. 2011 Feb 4;144(3):389-401.

Insert

2xPH(Osh2) fusion

Tags

GFP

Expression

Yeast

Promoter

PRC1

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
pyEvolvR (enCas9-PolI5M)

Plasmid

#158073

Purpose

Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber

Article

Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

Insert

enCas9-PolI5M

Expression

Bacterial and Yeast

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pSFS2A-CaKan

Plasmid

#189565

Purpose

Codon optomized Kan resistance gene in the pSFS2A flipper backbone for genetic manipulation of Candida albicans using G418 selection

Depositor

Richard Bennett

Article

Park et al mSphere. 2022 Aug 15:e0034722. doi: 10.1

Insert

CaKan

Expression

Yeast

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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FRP2370

Plasmid

#127576

Purpose

Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Leu2MX selection marker

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1

Use

Synthetic Biology; Shuttle vector

Expression

Yeast

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pHIS3p:yomWasabi-Tub1+3'UTR::URA3

Plasmid

#50637

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

yomWasabi-Tub1

Use

Yeast expression

Tags

yomWasabi

Promoter

HIS3p

Available Since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pHIS3p:yomWasabi-Tub1+3'UTR::HPH

Plasmid

#50631

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

yomWasabi-Tub1

Use

Yeast expression

Tags

yomWasabi

Promoter

HIS3p

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pHIS3p:yomWasabi-Tub1+3'UTR::LEU2

Plasmid

#50643

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

yomWasabi-Tub1

Use

Yeast expression

Tags

yomWasabi

Promoter

HIS3p

Available Since

Dec. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pJW1670

Plasmid

#112044

Purpose

CEN/ARS plasmid for GAL-inducible OLE1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

OLE1

Tags

V5-TEV-EGFP

Expression

Yeast

Mutation

P319T

Promoter

GAL1

Available Since

May 28, 2022

Availability

Academic Institutions and Nonprofits only

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pPTK021-7-PARS

Plasmid

#84990

Purpose

Type 7, Pichia autonomously replicating sequence

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Pichia autonomously replicating sequence

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pYD1-mSA

Plasmid

#39865

Depositor

Sheldon Park

Article

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin

Tags

Aga2, FLAG, and HA

Expression

Yeast

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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HSP104-b/Leu(WT)

Plasmid

#1156

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

HSP104 (HSP104 Budding Yeast)

Tags

GAL1:10 promoter

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pWS3865

Plasmid

#185742

Purpose

Sub-array backbone 1/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pFS554

Plasmid

#242854

Purpose

This plasmid will be used to replace the promoter of a gene with the PenotetSW3 promoter at its endogenous locus

Depositor

Nick Rhind

Article

Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791

Inserts

TetR
mECitrine

Expression

Yeast

Promoter

CMV promoter and enotetSW3 promoter

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pFS553

Plasmid

#242853

Purpose

This plasmid will be used to replace the promoter of a gene with the PenotetSW2 promoter at its endogenous locus

Depositor

Nick Rhind

Article

Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791

Inserts

TetR
mECitrine

Expression

Yeast

Promoter

CMV promoter and enotetSW2 promoter

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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HIP2

Plasmid

#31436

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Huntingtin Interacting protein 2 (UBE2K Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only