We narrowed to 13,271 results for: sequence
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Plasmid#51287Purposeexpresses the rodent SINE, B2, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassetteDepositorInsertsine B2
TagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianPromoterenhancer from 7SL geneAvailable SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
tet_pLKO.1_puro_shLuc
Plasmid#136587PurposeExpresses an inducible short hairpin targeting firefly luciferase sequenceDepositorInsertshLuc
UseLentiviralExpressionMammalianAvailable SinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTrc99a-NoSS-TolC
Plasmid#155179PurposeExpresses signal sequence-less TolC as inclusion bodiesDepositorInsertTolC (tolC )
ExpressionBacterialMutationdeleted residues 1-20, residue 21 mutated to Met …PromotertrcAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Emi LC
Plasmid#113666PurposeFor transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with anti-FIX(a) and anti-FX(a) Emi HC's.DepositorInsertEmicizumab light chain
ExpressionMammalianPromoterCMV PromoterAvailable SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGM202T7
Plasmid#69993PurposeE. coli/Streptomyces expression vector containing the T7-promoterDepositorInsertsaphII
tsr
rep
sso
T7 promoter
dso
His-Orf
TagsN-terminal and C-terminal His tag encoding sequen…ExpressionBacterialAvailable SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-FLAG-hEcto/Tif1g
Plasmid#20902DepositorInsertEctodermin (TRIM33 Human)
TagsFLAGExpressionMammalianMutationN-terminal deletion of 37 aminoacids; Ectodermin …Available SinceApril 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDS275
Plasmid#34972PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations).DepositorInsertMid2(SS/TM)-GFP-LOVpep
ExpressionYeastMutationT406A, T407A, I532AAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFH2.30_Gag-MCP-dpol-T2A-eGFP
Plasmid#205538PurposeExpress HIV Gag-MCP-dpol-T2A-eGFPDepositorInsertHIV Gag-MCP-dpol
ExpressionMammalianMutationpol frameshift sequence recodedPromoterCMVAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MBII85-5'SS mut
Plasmid#67631PurposeMBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons. To improve snoRNA expression efficiency 5' splice site was mutated to consensus sequence.DepositorInsertMBII85 snoRNA
ExpressionMammalianMutationmutation in 5'ssAvailable SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCD/NL-BH∆1
Plasmid#41791PurposePackaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding RevDepositorInsertHIV-1 Gag/Pol and RRE sequences
ExpressionMammalianAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-I_peroxisome_C1
Plasmid#85065PurposeIn vivo visualization of the peroxisome (can be used for colocalization studies)DepositorInsertPeroxisomal targeting sequence
TagsmScarlet-iExpressionMammalianPromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-blueAKAR(T/A)
Plasmid#118471PurposeNegative-control mutant for blueAKAR biosensor.DepositorInsertblueAKAR(T/A)
Tags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTS2311-Tier1-spacer-PPGK-NLuc_TB
Plasmid#169552PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPPGK-NanoLuc Luciferase - mTagBFP fusion
ExpressionMammalianPromoterPPGKAvailable SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRelA'
Plasmid#175595PurposeTet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.DepositorInsertCatalytic domain of E.coli relA gene.
ExpressionBacterialMutationOnly the catalytic domain of the enzyme is used (…Available SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-C
Plasmid#183160PurposePlant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of mCherry.DepositorTypeEmpty backboneExpressionPlantAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV)
Plasmid#140160PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)
TagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianPromoterEF-1aAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH_SBP-EGFP-Ecadherin
Plasmid#65290PurposeEcadherin reporter only (no hook) (RUSH system)DepositorInsertEcadherin
UseLentiviralTagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…PromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC6A3
Plasmid#132160PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC6A3 (SLC6A3 Human)
ExpressionMammalianAvailable SinceOct. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pscAluYNF1
Plasmid#50934PurposeExpresses scAluY RNA in mammalian cells.DepositorInsertscAluYNF1
ExpressionMammalianMutationthe poly(A) linker and the right arm of the dimer…Promoter7SL RNA promoter (pol III)Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC19/eCFP-C
Plasmid#183156PurposePlant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eCFPDepositorTypeEmpty backboneExpressionPlantAvailable SinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only