We narrowed to 17,015 results for: EMB

1. RGG-BcLOV-mCh-SOS

   Plasmid

   #208278

   Purpose

   Expresses RGG-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

   Depositor

   Lukasz Bugaj

   Article

   Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

   Insert

   RGG-BcLOV-mCh-SOS
   

   Use

   Lentiviral

   Promoter

   pCMV

   Available Since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Fus-BcLOV-mCh-EGFR

   Plasmid

   #208281

   Purpose

   Expresses Fus-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

   Depositor

   Lukasz Bugaj

   Article

   Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

   Insert

   Fus-BcLOV-mCh-EGFR
   

   Use

   Lentiviral

   Promoter

   pCMV

   Available Since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLP-sfgfp

   Plasmid

   #209994

   Purpose

   Contains Level 0 Part: Coding sequences (sfGFP) for the construction of Level 1 plasmids

   Depositor

   Georg Fritz, Thorsten Mascher

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   sfGFP
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLPT1

   Plasmid

   #209995

   Purpose

   Contains Level 0 Part: Terminator (TB1006) for the construction of Level 1 plasmids

   Depositor

   Georg Fritz, Thorsten Mascher

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   Terminator (TB1006)
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLPS1

   Plasmid

   #209992

   Purpose

   Contains Level 0 Part: Ribosome binding site (RB0034) for the construction of Level 1 plasmids

   Depositor

   Georg Fritz, Thorsten Mascher

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   RBS (RB0034)
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKE011

   Plasmid

   #207912

   Purpose

   Level 0 for gRNA in C4 module (5' GCAG-3' TCAG)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU3-AarI-scaffold-term
   

   Use

   CRISPR

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETmsGFPSyt1(80 JuxtaK-142)

   Plasmid

   #209013

   Purpose

   Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Tags

   6xHis-msfGFP

   Expression

   Bacterial

   Mutation

   aa 80-142 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETSyt1C2AB(143-421)msGFP

   Plasmid

   #209010

   Purpose

   Recombinant protein expression of truncated syt1 tagged with GFP

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Tags

   6xHis and msfGFP

   Expression

   Bacterial

   Mutation

   aa 143-421 only

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETSUMOSyt1C2AB(80 JuxtaK-421)

   Plasmid

   #209008

   Purpose

   Recombinant protein expression of truncated syt1 construct with Juxta K mutations

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFSynWSyt1C2AB(143-421)msGFP

    Plasmid

    #209015

    Purpose

    Overexpression of truncated syt1 tagged with GFP in mammalian cells

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    aa 143-421 only

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS018

    Plasmid

    #207911

    Purpose

    Level 0 for gRNA in C3 module (5' TTCG-3' GCAG)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU6-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS017

    Plasmid

    #207909

    Purpose

    Level 0 for gRNA in C1 module (5' GGCT-3' AGCC)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU6-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCS020

    Plasmid

    #207915

    Purpose

    Level 0 for gRNA in D3 module (5' TCCC-3' CTGC)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU6-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKE012

    Plasmid

    #207914

    Purpose

    Level 0 for gRNA in AD2 module (5' TGAC-3' TCCC)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU3-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKE010

    Plasmid

    #207910

    Purpose

    Level 0 for gRNA in C2 module (5' AGCC-3' TTCG)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU3-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCS019

    Plasmid

    #207913

    Purpose

    Level 0 for gRNA in D1 module (5' TCAG-3' TGAC)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU6-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMx/ATP10D-HA

    Plasmid

    #209234

    Purpose

    Mammalian expression of ATP10D

    Depositor

    Hye-Won Shin

    Article

    Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

    Insert

    ATP10D (ATP10D Human)
    

    Use

    Retroviral

    Tags

    HA

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pL0_DE-Per_sig_pep

    Plasmid

    #202152

    Purpose

    Level 0 plasmid containing a peroxisome signal peptide sequence with DE overhangs used to build level 1 constructs.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    Peroxisome signal peptide
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Oct. 19, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pSC-cUnaG

    Plasmid

    #207631

    Purpose

    A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    photocaged SpyCatcher-cUnaG
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Amber stop codon at SC's critical lysine

    Promoter

    T7

    Available Since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSC-LPETG

    Plasmid

    #207629

    Purpose

    A plasmid encoding photocaged SpyCatcher (pSC) with a C-terminal Sortase recognition sequence.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    photocaged SpyCatcher-LPETG
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Amber stop codon at SC's critical lysine

    Promoter

    T7

    Available Since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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