We narrowed to 9,878 results for: EPO
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Plasmid#84636PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub(TA)-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSI-check2-CUL1-3'UTR
Plasmid#97156PurposeFor dual luciferase report assayDepositorAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
mito-yHS1-M7A
Plasmid#159164PurposeMitochondrial labile heme reporterDepositorInsertmito-yHS1-M7A
UseTagsCOX4 pre-sequenceExpressionYeastMutationMutated Met 7 of the cyt b562 module of mito-yHS1…PromoterGPDAvailable SinceSept. 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
LSB-hsa-miR-101-3p
Plasmid#103165PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-101-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-101-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL4.24-Cd36-enhancer1
Plasmid#138573PurposeFirefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17835541_17837355DepositorAvailable SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-GL-DSCP
Plasmid#83338Purposefor Gateway cloning of enhancer elements upstream of the DSCP and GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsGFP::Luciferase(nls)ExpressionInsectMutationPromoterDrosophila Synthetic Core PromoterAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pRP(Exp)-CMV> ORF_924bp (Azgp1):T2A:dTomato:IRES:Puro
Plasmid#110830PurposeExpresses zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter where both sequences are separated by a T2A self-cleaving peptide sequence as a result, the dT is not fused with the ZAG protein.DepositorInsertAZGP1 (Azgp1 Mouse)
UseNon-viral gene expression vectorTagsT2A and dTomatoExpressionMutationPromoterCMVAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-hDcp2 E147/8Q
Plasmid#72217Purposebacterial expression of GST-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)DepositorInsertDcp2 (DCP2 Human)
UseTagsGSTExpressionBacterialMutationE147Q, E148Q and F404L (please see depositor'…PromotertacAvailable SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1-promoter-mIL-6
Plasmid#109296PurposeIL-6 promoter reporter constructDepositorInsertmurine IL-6 promoter (Il6 Mouse)
UseTagsExpressionMammalianMutationCMV promoter in pEGFP-N1 replaced with murine IL-…Promotermurine IL-6 promoterAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI
Plasmid#140202PurposeCRISPRa proof-of-concept test target with fluorescent reporter. PelcA is fused to mCherry, with low basal expression in Aspergillus nidulans. Fungal vector with AMA1 and pyrG selection marker.DepositorInsertmCherry
UseCRISPR and Synthetic Biology; Proof-of-concept te…TagsExpressionMutationG174DPromoterPelcAAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VPH-T2A-GFP-IRES-Neo
Plasmid#102890PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21c-His-Xa-Gluc-Xa-C9D-TGinsert
Plasmid#124660Purposereporter protein which emits bright blue fluorescenceDepositorInsertGaussia luciferase-C9D-TGinsert
UseTagsIEGRTMGDDDGDDDGDDD(FactorXa,TMG,C9D-Tag) and MHHH…ExpressionBacterialMutationchanged glutamic acid 100 to alanine, and glycine…PromoterT7 promoterAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo
Plasmid#102892PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-516b-5p
Plasmid#103601PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-516b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-516b-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Tet1 promoter A
Plasmid#63881PurposeLuciferase reporter plasmid used to study transcriptional control of murine Tet1DepositorInsertTet1 promoter fragment A
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable SinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Tet1 promoter B
Plasmid#63882PurposeLuciferase reporter plasmid used to study transcriptional control of murine Tet1DepositorInsertTet1 promoter fragment B
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-mR2-TUBA1B_sgRNA
Plasmid#183888PurposepX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.DepositorAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1Gh
Plasmid#44507DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo
Plasmid#102891PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-P300-T2A-GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only