We narrowed to 14,584 results for: pET

1. pET29b-mVenus-dspB(E184Q W330Y)-6xHis

   Plasmid

   #176577

   Purpose

   Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Use

   Tags

   Hexahistidine tag and mVenus

   Expression

   Bacterial

   Mutation

   E184Q W330Y

   Promoter

   T7

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-anticalin-FLN-ELP-His-ybbr

   Plasmid

   #168039

   Purpose

   Expresses anticalin targeting CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   3xELP linker, 6x Histag, ddFLN4 (C18S), and ybbr …

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-SdrG-FLN-ELP-His-ybbr

   Plasmid

   #168047

   Purpose

   Expresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
   

   Use

   Tags

   3xELP linker, 6x Histag, ddFLN4, and ybbr tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)

   Plasmid

   #91818

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutations

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed lysine 1355 and lysine 1435 to alanines; …

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-Synapsin-YPET-3XNLS-optoNES-WPRE

   Plasmid

   #159947

   Purpose

   optical regulation of nuclear export

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   YPET-3XNLS-optoNES
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-Synapsin-NLS-YPET-optoNES-WPRE

   Plasmid

   #159942

   Purpose

   optical regulation of nuclear export

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   NLS-YPET-optoNES
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-Synapsin-NLS-YPET-optoNES.F434L-WPRE

   Plasmid

   #159975

   Purpose

   optical regulation of nuclear export with shorter relaxation time

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   NLS-YPET-optoNES.F434L
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   F434L

   Promoter

   hSyn1

   Available since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-Synapsin-NLS-YPET-optoNES.V416I-WPRE

   Plasmid

   #159973

   Purpose

   optical regulation of nuclear export with extended relaxation time

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   NLS-YPET-optoNES.V416I
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   V416I

   Promoter

   hSyn1

   Available since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-Synapsin-NLS-YPET-optoNES.I427V-WPRE

   Plasmid

   #159974

   Purpose

   optical regulation of nuclear export with shorter relaxation time

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   NLS-YPET-optoNES.I427V
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   I427V

   Promoter

   hSyn1

   Available since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-45b-TBP(38Q)-IDR-mCherry

    Plasmid

    #145283

    Purpose

    Bacterial Expression of 6xHis TBP(38Q) IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    TBP(38Q)-IDR (TBP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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