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actc1b-mKate2-rab14

Plasmid

#109600

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab14

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab14 (rab14 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-CDC42

Plasmid

#109531

Purpose

Muscle specific zebrafish expression of mKate2 fused to CDC42

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CDC42 (cdc42 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-CDC42-DN

Plasmid

#109532

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative CDC42

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CDC42 (cdc42 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

DN

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDK480

Plasmid

#134197

Purpose

Mammalian expression of Flip in SKAP and IRES GFP

Depositor

Iain Cheeseman

Article

Kern et al J Cell Biol. 2016 May 9;213(3):315-28. d

Insert

untagged AltSKAP (hardened to G5siRNA) (KNSTRN Human)

Tags

IRES-GFP

Expression

Mammalian

Mutation

Alternate Start, G5 siRNA hardened

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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IX601: pMVP (L3-L2) FLAG epitope tag + polyA

Plasmid

#121751

Purpose

pMVP L3-L2 entry plasmid, contains FLAG epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

FLAG epitope tag + polyA

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-1gamma

Plasmid

#124141

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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FB030

Plasmid

#119714

Purpose

Terminator paf from P. chrysogenum domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

Terminator paf

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSG75-β2AR-T4L-sfGFP

Plasmid

#102461

Purpose

Expresses a fusion protein of β2 adrenergic receptor/T4 lysozyme chimera and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.

Depositor

Richard Murray

Article

Guo et al bioRxiv 104455

Insert

β2AR

Tags

His tag and sfGFP

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-SMAD3

Plasmid

#79507

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human SMAD3 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

SMAD3 (SMAD3 Human)

Use

Gateway Cloning

Tags

FLAG

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 sgATXN1L-1

Plasmid

#74970

Purpose

sgATXN1L-1, puromycin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ATXN1L (ATXN1L Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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Ntn5.z-Fc-His

Plasmid

#72109

Purpose

Expresses the entire Netrin 5, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntn5 (Ntn5 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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hEBI3 pEF6-Flag (MJS_003)

Plasmid

#72491

Purpose

Expression of human EBI3 in mammalian cells (Flag tag)

Depositor

Matthew Sweet

Article

Ramnath et al Immunol Cell Biol. 2015 Oct;93(9):771-9.

Insert

EBI3 (EBI3 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Ntn5.y-AP-His

Plasmid

#71982

Purpose

Expresses the entire Netrin 5, isoform y protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntn5.y (Ntn5 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2016

Availability

Academic Institutions and Nonprofits only

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Ntn5.z-AP-His

Plasmid

#71983

Purpose

Expresses the entire Netrin 5, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntn5.z (Ntn5 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 YWHAB

Plasmid

#67063

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

YWHAB (YWHAB Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
MXS_PGK::tTA2-bGHpA

Plasmid

#62448

Purpose

MXS_chaining vector with PGK::tTA2-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the tetracycline transactivator with PGK promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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Wnt1 Non-STOP

Plasmid

#35887

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt1 (WNT1 Human)

Use

Gateway Cloning

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNAFlagMenin(S543A)

Plasmid

#32083

Depositor

Matthew Meyerson

Article

Francis et al PLoS One. 2011 . 6(1):e16119.

Insert

Menin isoform 2 (MIR302B Human)

Tags

Flag

Expression

Mammalian

Mutation

S543A

Promoter

CMV

Available Since

Feb. 24, 2012

Availability

Academic Institutions and Nonprofits only
SG-3'REL

Plasmid

#27257

Depositor

Thomas Gilmore

Article

Starczynowski et al Oncogene. 2003 Oct 9. 22(44):6928-36.

Insert

REL (REL Human)

Tags

GAL4

Expression

Mammalian

Mutation

Contains aa 278-587 of REL

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only
GST HP1 gamma deltaC

Plasmid

#24077

Depositor

Naoko Tanese

Article

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 gamma (CBX3 Human)

Tags

GST

Expression

Bacterial

Mutation

Missing the C-terminal 25 amino acids

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only