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Plasmid#62816PurposeBacterial constitutive gRNA expression plasmidDepositorInsertgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPN25Available sinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
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mU6-sgSik1_2nd-hU6-sgSik3_2nd
Plasmid#177219PurposeExpresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinaseDepositorInsertsgSik1_2nd/sgSik3_2nd
UseLentiviralTagsExpressionMutationPromotermU6/hU6Available sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
mU6-sgSik1_2nd-hU6-sgSik3_1st
Plasmid#177218PurposeExpresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinaseDepositorInsertsgSik1_2nd/sgSik3_1st
UseLentiviralTagsExpressionMutationPromotermU6/hU6Available sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRHOXF2B.1.0-gDNA
Plasmid#132462PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertRHOXF2B (RHOXF2B Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBsacA
Plasmid#158650PurposeConstitutive transcription of sacA crRNADepositorInsertsacA crRNA
UseCRISPR and Synthetic Biology; Shuttle vector baci…TagsExpressionBacterialMutationPromoterPvegAvailable sinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfb13193
Plasmid#219868PurposeThe base plasmid of TUNEYALI for TF03DepositorInsertContains gRNA targeting TF03 (YALI1_B05350g) and homologous arm matching TF03
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13205
Plasmid#219880PurposeThe base plasmid of TUNEYALI for TF15DepositorInsertContains gRNA targeting TF15 (YALI1_B29562g) and homologous arm matching TF15
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT2/shαSMA/GFP4_Seq2/clone1
Plasmid#201402PurposeKnockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertalpha-SMA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SGEP-sh-Hs-PHGDH-1968
Plasmid#188673PurposeshRNADepositorInsertshPHGDH (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
SGEP-sh-Hs-PHGDH-1967
Plasmid#188672PurposeshRNADepositorInsertshPHGDH (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
mU6-sgSik1_2nd-hU6-sgNT
Plasmid#177213PurposeExpresses a Sik1-targeting (mU6) and a non-targeting (hU6) gRNAs and Cre recombinaseDepositorInsertsgSik1_2nd/sgNT1
UseLentiviralTagsExpressionMutationPromotermU6/hU6Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pADsacA
Plasmid#158649PurposeConstitutive transcription of sacA crRNA and GFPDepositorInsertsacA crRNA
UseCRISPR and Synthetic Biology; Shuttle vector baci…TagsExpressionBacterialMutationPromoterPvegAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHTganA-Cpf1
Plasmid#158647PurposeConstitutive transcription of FnCpf1 and ganA crRNADepositorInsertCpf1, ganA crRNA
UseCRISPR and Synthetic Biology; Shuttle vector baci…TagsExpressionBacterialMutationPromoterP43Available sinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
ipUSEPR-sg-Hs-PHGDH-int2
Plasmid#188682Purposecontrol sgRNADepositorInsertsgPHGDH intron control (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICSL4723_ CUL3
Plasmid#126891PurposeGenome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.DepositorInsertWheat_live_Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTRY4_ CUL3
Plasmid#126903PurposeGateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.DepositorInsertWheat_live_Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
1781_pAAV-U6-Ai9-Sa-gRNA1-CB-SACas9-HA-OLLAS-spA_C
Plasmid#135979PurposegRNA against the left loxP site of the Ai9 alleleDepositorInsertAi9 gRNA1
UseAAV, CRISPR, and Mouse TargetingTagsHAExpressionMutationPromoterU6Available sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pML107-RER2
Plasmid#232884PurposePlasmid expressing Cas9 and gRNA AGAATCGCATCTCTACACGG which targets the RER2 gene.DepositorInsertRER2 gRNA (RER2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_TDH2
Plasmid#166082PurposePlasmid for constituive spCas9 and tet-inducible TDH2 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertTDH2 (TDH2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only