We narrowed to 2,998 results for: Gateway donor

1. pDD362

   Plasmid

   #137023

   Purpose

   SapTrap HaloTag-GLO donor

   Depositor

   Bob Goldstein, Daniel Dickinson

   Insert

   C. elegans GLO-optimized Halo Tag
   

   Use

   CRISPR; Saptrap donor for cloning repair templat…

   Tags

   C. elegans GLO-optimized Halo Tag

   Expression

   Worm

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF 1-PNH-4

   Plasmid

   #20083

   Depositor

   Antonio Richart

   Insert

   PNH promoter
   

   Use

   Synthetic Biology; Plant gateway donor vector

   Available Since

   March 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC84-pCR8-dCas9

   Plasmid

   #48218

   Purpose

   dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A)
   

   Use

   CRISPR; Gateway donor

   Tags

   HA Tag

   Mutation

   D10A;H840A

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221-rap1-K9R

   Plasmid

   #84527

   Purpose

   This plasmid can be used as a donor for subcloning into Gateway destination vectors.

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Gateway donor plasmid

   Mutation

   K(43,61,69,117,240,246,527,582,651)R

   Promoter

   none

   Available Since

   Jan. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pDONR221-rap1-K462-463-651R

   Plasmid

   #84564

   Purpose

   This plasmid can be used as a donor for subcloning into Gateway destination vectors.

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Gateway donor plasmid

   Mutation

   K462-463-651R

   Promoter

   none

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-rap1-K43-117-118R

   Plasmid

   #84565

   Purpose

   This plasmid can be used as a donor for subcloning into Gateway destination vectors.

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Gateway donor plasmid

   Mutation

   K43-117-118R

   Promoter

   none

   Available Since

   Oct. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR-IAA1_no stop

   Plasmid

   #83847

   Purpose

   Gateway donor vector for IAA1 with no stop codon

   Depositor

   Jennifer Nemhauser

   Article

   Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

   Insert

   IAA1 (IAA1 Mustard Weed)
   

   Use

   Gateway donor vector

   Mutation

   no stop codon

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221-deltaUBA-short-UBASH3A-Ctag

   Plasmid

   #195397

   Purpose

   Human short-form UBASH3A lacking the UBA domain in Gateway pDONR221 (for C-terminal tag)

   Depositor

   Patrick Concannon

   Insert

   UBASH3A (UBASH3A Human)
   

   Use

   Gateway donor vector

   Mutation

   UBASH3A mutant lacking the UBA domain

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221-deltaUBA-short-UBASH3A-Ntag

   Plasmid

   #195398

   Purpose

   Human short-form UBASH3A lacking the UBA domain in Gateway pDONR221 (for N-terminal tag)

   Depositor

   Patrick Concannon

   Insert

   UBASH3A (UBASH3A Human)
   

   Use

   Gateway donor vector

   Mutation

   UBASH3A mutant lacking the UBA domain

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

    Plasmid

    #175573

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

    Plasmid

    #175574

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPV475

    Plasmid

    #42928

    Depositor

    James Lok

    Insert

    attR2::Ss-era-1 terminator (598 bp)::attL3
    

    Use

    Multi-site gateway donor plasmid

    Available Since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAC147-pCR8-dCas9VP64

    Plasmid

    #48219

    Purpose

    dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression 

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP64 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP64

    Mutation

    D10A;H840A

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC148-pCR8-dCas9VP96

    Plasmid

    #48220

    Purpose

    dCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP96 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP96

    Mutation

    D10A;H840A

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC1-pCR8-dCas9VP48

    Plasmid

    #48214

    Purpose

    dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP48 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP48

    Mutation

    D10A;H840A

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. Fragment1 with TLS2

    Plasmid

    #196983

    Purpose

    Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Intermediate fragment for assembly of gRNA-TLS2 construct
    

    Use

    Gateway-compatible entry vector

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Fragment 1 Null

    Plasmid

    #198729

    Purpose

    Gateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    gRNA expression cassette in pENTR4
    

    Use

    Gateway-compatible entry vector

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAC149-pCR8-dCas9VP160

    Plasmid

    #48221

    Purpose

    dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP160

    Mutation

    D10A;H840A nuclease-deficient

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. GK Rab11A p201

    Plasmid

    #44704

    Depositor

    Geri Kreitzer

    Insert

    RAB11A (RAB11A Human)
    

    Use

    Gateway donor vector

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pPV476

    Plasmid

    #42929

    Depositor

    James Lok

    Insert

    attR2::egfp (870 bp)::taa::Ss-era-1 terminator (598 bp)::attL3
    

    Use

    Multi-site gateway donor plasmid

    Available Since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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