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pME-Cas9

Plasmid

#64237

Purpose

expresses codon optimized Cas9 in zebrafish

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Insert

codon optimized Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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UAS::PTPRFa.1-DN-GFP

Plasmid

#37110

Depositor

Alvaro Sagasti

Article

Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

Insert

UAS::PTPRFa.1-DN-GFP (ptprf Zebrafish)

Use

Tol2 gateway expression vector

Tags

EGFP

Expression

Mutation

deleted amino acids 1326-1894

Promoter

UAS-E1b

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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UAS::PTPRDb-DN-GFP

Plasmid

#37112

Depositor

Alvaro Sagasti

Article

Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

Insert

UAS::PTPRDb-DN-GFP (ptprdb Zebrafish)

Use

Tol2 gateway destination vector

Tags

EGFP

Expression

Mutation

deleted cytodomain, including phosphatase domains…

Promoter

UAS

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pPEPT1

Plasmid

#195001

Purpose

Production of peptides in E. coli as GB1 fusion

Depositor

Marko Hyvönen

Article

Pantelejevs et al Chem Sci. 2023 Nov 21;14(47):13915-13923

Type

Empty backbone

Use

Tags

His8-tag and StrepTagII-GB1

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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T7-VEE-OKS-iM

Plasmid

#58972

Purpose

RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Inserts

Oct4 (POU5F1 Human)
Klf4 (KLF4 Human)
Sox2 (SOX2 Human)
cMyc (MYC Human)

Use

Venezuelan equine encephalitis (vee) virus rna re…

Tags

Expression

Mammalian

Mutation

Promoter

26S subgenomic promoter and IRES

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pLXI_TRC403 SMARCB1 var 2

Plasmid

#111185

Purpose

Inducible vector (V5 tagged) with SMARCB1 variant 2

Depositor

William Hahn

Article

Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

Insert

BAF47 (SMARCB1 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

TRE

Available since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pQTEV-SCP2

Plasmid

#31336

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

sterol carrier protein 2 (SCP2 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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UAS::PTPRFb-DN-GFP

Plasmid

#37111

Depositor

Alvaro Sagasti

Article

Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

Insert

UAS::PTPRFb-DN-GFP (ptprfb Zebrafish)

Use

Tol2 gateway expression vector

Tags

EGFP

Expression

Mutation

Deleted aa1386-1909 corresponding to phosphatase …

Promoter

10xUAS

Available since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

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pDestTol2CG2-mnx1:ReaChR-citrine

Plasmid

#169046

Purpose

Destination transgenesis vector for expressing ReaChR fused with citrine in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

Depositor

Paola Arlotta

Article

Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

Insert

ReaChR-Citrine

Use

Tags

Citrine

Expression

Mammalian

Mutation

Promoter

3xMnx1

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-natMX6-Purg1-3FLAG

Plasmid

#19355

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Aug 26. ():.

Insert

urg1 promoter

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pFA6a-hphMX6-Purg1-3FLAG

Plasmid

#19356

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Aug 26. ():.

Insert

urg1 promoter

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-KanMX6-Purg1-3FLAG

Plasmid

#19354

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Aug 26. ():.

Insert

urg1 promoter

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pFA6a-bleMX6-Purg1-3FLAG

Plasmid

#19357

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Aug 26. ():.

Insert

urg1 promoter

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pFA6a-his3-P41nmt1-3FLAG

Plasmid

#19340

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Aug 26. ():.

Insert

his3 (his3 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pFA6a-his3-P81nmt1-3FLAG

Plasmid

#19341

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Aug 26. ():.

Insert

his3 (his3 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pFA6a-his3-P3nmt1-3FLAG

Plasmid

#19339

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Aug 26. ():.

Insert

his3 (his3 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pUR-sap1+

Plasmid

#15984

Depositor

Eishi Noguchi

Article

Noguchi et al Genetics. 2007 Feb . 175(2):553-66.

Insert

Sap1 (sap1 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2007

Availability

Academic Institutions and Nonprofits only
pFA6a-5FLAG-hphMX6

Plasmid

#19342

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Aug 26. ():.

Insert

hphMX6

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pMK90

Plasmid

#13323

Depositor

Martin Gellert

Article

Mizuuchi et al J Biol Chem. 1984 Jul 25. 259(14):9199-2

Insert

gyrA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pMK47

Plasmid

#13324

Depositor

Martin Gellert

Article

Mizuuchi et al J Biol Chem. 1984 Jul 25. 259(14):9199-2

Insert

gyrB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only