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pET28b-FOXP2_P3

Plasmid

#122647

Purpose

Expresses truncated variant (aa367-aa714) of human FOXP2 in bacterial cells

Depositor

Hendrik Dietz

Article

Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

Insert

Homo sapiens forkhead box P2 (FOXP2 Human)

Use

Tags

His-tag and Strep-tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-FOXP2_P4

Plasmid

#122648

Purpose

Expresses truncated variant (aa503-aa714) of human FOXP2 in bacterial cells

Depositor

Hendrik Dietz

Article

Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

Insert

Homo sapiens forkhead box P2 (FOXP2 Human)

Use

Tags

His-tag and Strep-tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-H3

Plasmid

#124435

Purpose

Chimeric ETS domain: H3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Use

Tags

6xHis Tag

Expression

Bacterial

Mutation

Residues 225 to 239 replaced with corresponding s…

Promoter

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP1-N

Plasmid

#128310

Purpose

Express hNABP1 N-terminus (1–141 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

hNABP1 N-terminal domain (OB fold, 1–141 aa) (NABP1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

N-terminal domain (OB fold, 1–140 aa)

Promoter

T7

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP2-C

Plasmid

#128309

Purpose

Express hNABP2 C-terminus(122–211 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

hNABP2 C-terminal domain (122–211 aa) (NABP2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

C-terminal domain (122–211 aa)

Promoter

T7

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-INTS3-C

Plasmid

#128642

Purpose

Express INTS3 C-termini (514-1042 aa) in E. coli with N-terminal and C-terminal His tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 C-termini (INTS3 Human)

Use

Tags

His tag on N and C-terminal

Expression

Bacterial

Mutation

INTS3 C-termini (514–1042 aa)

Promoter

T7

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-H3/S3

Plasmid

#124627

Purpose

Chimeric ETS domain: H3/S3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 237 to 241 replaced with corresponding s…

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28B-PU.1-ETS-S3

Plasmid

#124550

Purpose

Chimeric ETS domain: S3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 242 to 246 replaced with corresponding s…

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-PU.1-ETS-H2

Plasmid

#123358

Purpose

Chimeric ETS domain: H2 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 207 to 215 replaced with corresponding s…

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-MetN

Plasmid

#114458

Purpose

Expression of hexahistidine-tagged AceS methylase in E. coli

Depositor

Patrick Caffrey

Article

Sheehan et al Mol Biosyst. 2017 May 2;13(5):866-873. d

Insert

aceS

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-YJR129c

Plasmid

#85118

Purpose

bacterial expression of YJR129c

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EFM3 (EFM3 Budding Yeast)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-Prp2

Plasmid

#111348

Purpose

protein purification

Depositor

Christine Guthrie

Insert

PRP2

Use

Tags

6x His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(WT)

Plasmid

#112720

Purpose

Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(WT)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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Prp39-pET21b

Plasmid

#111286

Purpose

Prp39 expression plasmid

Depositor

Christine Guthrie

Article

Price et al RNA. 2014 Jan;20(1):46-60. doi: 10.1261/

Insert

PRP39

Use

Tags

his6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-Cwc25

Plasmid

#111341

Purpose

his tagged Cwc25

Depositor

Christine Guthrie

Insert

CWC25

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET28B-Cwc25

Plasmid

#111340

Purpose

his tagged Cwc25

Depositor

Christine Guthrie

Insert

CWC25

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Prp40-pET21b

Plasmid

#111287

Purpose

Prp40 expression plasmid

Depositor

Christine Guthrie

Article

Price et al RNA. 2014 Jan;20(1):46-60. doi: 10.1261/

Insert

PRP40

Use

Tags

his6

Expression

Bacterial

Mutation

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-HypD

Plasmid

#110288

Purpose

Expresses t-4-hydroxy-L-proline dehydratase in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

t-4-Hydroxy-L-proline Dehydratase

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-PDAE

Plasmid

#110180

Purpose

Expresses propanediol dehydratase activating enzyme in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase Activating Enzyme

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-PD-H166Q

Plasmid

#110179

Purpose

Expresses propanediol dehydratase mutant H166Q in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase

Use

Tags

His6 and S-tag

Expression

Bacterial

Mutation

H166Q mutant

Promoter

T7

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge