We narrowed to 883 results for: golden gate assembly
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Plasmid#130956PurposeEngineered sgRNA-LEB2 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB2
UseSynthetic BiologyExpressionBacterialPromoterAnderson promoter J23100Available SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY132
Plasmid#130960PurposeEngineered sgRNA-LEB3 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB3
UseSynthetic BiologyExpressionBacterialPromoterAnderson promoter J23100Available SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY130
Plasmid#130952PurposeEngineered sgRNA-LEB1 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB1
UseSynthetic BiologyExpressionBacterialPromoterAnderson promoter J23100Available SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBTK151
Plasmid#183127PurposeBTK Type 4 reverse promoter part for golden gate assemblyDepositorInsertReverse CP25 promoter and forward rpoC terminator for dsRNA expression
UseSynthetic BiologyAvailable SinceMay 10, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK150
Plasmid#183126PurposeBTK Type 2 promoter part for golden gate assemblyDepositorInsertReverse rnpB-T1 terminator and forward CP25 promoter for dsRNA expression
UseSynthetic BiologyAvailable SinceMay 10, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYPQ143
Plasmid#69295PurposeGolden Gate recipient and Gateway entry vector; assembly of 3 gRNAsDepositorTypeEmpty backboneUseCRISPRAvailable SinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKG1334_pHarbor-PiggyBac_backbone
Plasmid#239705PurposePaqCI Golden Gate assembly plasmid encoding the backbone for PiggyBac-mediated integrationDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR R4 ATCC LacZ GCTT
Plasmid#173668PurposeDestination vector for Golden Gate Cloning assemblies, blue-white screeningDepositorTypeEmpty backboneExpressionBacterial and InsectAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAcCAST-sgRNA_entry (CJT83)
Plasmid#181785PurposeExpresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertAcTnsB, AcTnsC, AcTniQ, AcCas12k
ExpressionBacterialPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShoCAST-sgRNA_entry (BO1)
Plasmid#181786PurposeExpresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
ExpressionBacterialPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNM3746
Plasmid#154109PurposeloxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vectorDepositorInsertsLoxP-SEC-LoxP
FRT::rpl-28p::GFP::His-58::FRT3
mex-5p::FLP D5::sl2::mNG::glh2 3'
UseCre/Lox; RmceExpressionWormPromoternot applicableAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-BsaI-EGFP
Plasmid#123136PurposeGolden Gate acceptor vector for the assembly of EGFP-based mammalian recombinase reporters.DepositorInsertEGFP, mRFP
UseSynthetic BiologyExpressionMammalianPromotercBAAvailable SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFUS_A8
Plasmid#99882PurposeRecipient vector for Golden Gate TALE cloning system. Used to assemble 8 TALE repeats.DepositorTypeEmpty backboneUseTALENAvailable SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
p1300-dCas9-HV5
Plasmid#239479PurposeAn intermediate vector is used to assemble three gRNAs via Golden Gate cloning.DepositorInsertUBQ-dCAS9
UseCRISPRExpressionPlantAvailable SinceDec. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG1179_pPV1-EFS
Plasmid#239690PurposeBsaI Golden Gate assembly plasmid encoding the EFS promoterDepositorInsertEFS promoter
UseSynthetic BiologyAvailable SinceOct. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG0586_pPV2-mRuby2
Plasmid#239695PurposeBsaI Golden Gate assembly plasmid encoding the mRuby2 CDSDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2039_pPV1-CAG
Plasmid#239686PurposeBsaI Golden Gate assembly plasmid encoding the CAG promoterDepositorInsertCAG promoter
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330-BbsI-PITCh
Plasmid#127875PurposeFor user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assemblyDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEC-red
Plasmid#196040PurposeAllows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate AssemblyDepositorTypeEmpty backboneUseCRISPR and Synthetic Biology; Grna cloningExpressionYeastAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHelper_ShCAST_sgRNA
Plasmid#127921PurposeExpresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only