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pTEI161

Plasmid

#126211

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP11

Use

Binary, module 1

Tags

Expression

Plant

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-K295TAG-W340A-S233H-T237H.pcDNA3-k

Plasmid

#127400

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon, W340A,…

Promoter

CMV

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z19

Plasmid

#124791

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry-mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry-mNeonGreen

Use

Pcr template

Tags

mCherry-mNeonGreen

Expression

Mutation

Promoter

None

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pN_35S/CTP-fluoA

Plasmid

#117990

Purpose

Chloroplast-targeted expression of CURT_fluoA controlled by the CaMV 35S promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CTP-CURT_fluoA

Use

Synthetic Biology; Plant/bacteria binary vector

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pN_35S/CTP-fluoB

Plasmid

#117991

Purpose

Chloroplast-targeted expression of CURT_fluoB controlled by the CaMV 35S promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CTP-CURT_fluoB

Use

Synthetic Biology; Plant/bacteria binary vector

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pN_35S/CTP-fluoE

Plasmid

#117992

Purpose

Chloroplast-targeted expression of CURT_fluoE controlled by the CaMV 35S promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CTP-CURT_fluoE

Use

Synthetic Biology; Plant/bacteria binary vector

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pN_35S/CURT_flouB

Plasmid

#117995

Purpose

Expression of CURT_flouB controlled by the CaMV 35S promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CURT_flouB

Use

Yeast/bacteria binary vector

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pIVT/Acrosin-GFP-GPI

Plasmid

#122141

Purpose

For making acrosin-GFP-GPI RNA

Depositor

Carmen Williams

Article

Winuthayanon et al Elife. 2015 Dec 1;4:e10453. doi: 10.7554

Insert

acrosin-GFP-GPI

Use

For in vitro transcription

Tags

Expression

Mutation

Promoter

T7

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z32

Plasmid

#120075

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with S-Tag

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

S-Tag

Use

Pcr template

Tags

S-Tag

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z30

Plasmid

#120073

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with VSV-G

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

VSV-G

Use

Pcr template

Tags

VSV-G

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z27

Plasmid

#120070

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 1xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

1xHA

Use

Pcr template

Tags

1xHA

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z24

Plasmid

#120067

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TAP

Use

Pcr template

Tags

TAP

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z10

Plasmid

#120053

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKOk

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKOk

Use

Pcr template

Tags

mKOk

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z01

Plasmid

#120044

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxBFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxBFP

Use

Pcr template

Tags

moxBFP

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P20

Plasmid

#120031

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 10xSuntag

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

10xSuntag

Use

Pcr template

Tags

10xSuntag

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P16

Plasmid

#120027

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Dronpa3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Dronpa3

Use

Pcr template

Tags

Dronpa3

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAL829

Plasmid

#119697

Purpose

precursor slow-folding maltodextrin binding protein, MBP or malE, with mutations Y283D, G289C and V347C

Depositor

Linda Randall

Article

Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

Insert

slow-folding maltose ABC transporter periplasmic binding protein, malE, with mutations Y283D, G289C and V347C

Use

Tags

none

Expression

Bacterial

Mutation

mutations Y283D, G289C and V347C

Promoter

T5

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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LI F-G Gent

Plasmid

#104522

Purpose

LI resistance gene module. Please note that the plasmid contains Apramycin selection, not Gentamycin.

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Apramycin resistance gene

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pQE80L TriCatcher-MMP

Plasmid

#112632

Purpose

Two SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcher

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

TriCatcher-MMP

Use

Tags

His6 and TEV cleavage site

Expression

Bacterial

Mutation

central matrix metalloproteinase-cleavable sequen…

Promoter

T5

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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GST-GNU-N

Plasmid

#112972

Purpose

Expresses fusion of GST and N terminal amino acids of GNU

Depositor

Terry Orr-Weaver

Article

Lee et al Genes Dev. 2003 Dec 1;17(23):2979-91. do

Insert

gnu (gnu Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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