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LENTICRISPR-TYMS_sgRNA1

Plasmid

#201628

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

TYMS (TYMS Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-TYMS_sgRNA2

Plasmid

#201629

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

TYMS (TYMS Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-UCK1_sgRNA1

Plasmid

#201630

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

UCK1 (UCK1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-UCK1_sgRNA2

Plasmid

#201631

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

UCK1 (UCK1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LENTICRISPR-UCK2_sgRNA1

Plasmid

#201632

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

UCK2 (UCK2 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRetroX-TRE3G_NT-GSDMD_I105N__C192A

Plasmid

#201176

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, I105N, C192A

Promoter

TRE3G

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NT-GSDMD_C192A

Plasmid

#201172

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, C192A

Promoter

TRE3G

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NT-GSDMD_I105N_C39A

Plasmid

#201175

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, I105N, C39A

Promoter

TRE3G

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shCol1a1/Scarlet_Seq1.2

Plasmid

#201404

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2/shCol1a1/Scarlet-Seq1.1

Plasmid

#201401

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2/Col1a1/GFP4_Seq 1.1

Plasmid

#201400

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRetroX-TRE3G_NT-GSDMD_C39A

Plasmid

#201171

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, C39A

Promoter

TRE3G

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pNIC28-HiBIT-WDR5

Plasmid

#200883

Purpose

Bacterial expression of N-terminal HiBIT-tagged human WDR5 protein

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

HiBIT-WDR5 (WDR5 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK BirA* 3xFLAG-CSE1L zeo

Plasmid

#192334

Purpose

Lentiviral expression vector for an inducible 3xFLAG-BirA 3xFLAG-CSE1L

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

BirA*-3xFLAG-CSE1L (CSE1L Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT BirA*-3xFLAG-CSE1L

Plasmid

#192312

Purpose

Gateway entry vector for an inducible 3xFLAG-BirA* 3xFLAG-CSE1L

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

BirA*-3xFLAG-CSE1L (CSE1L Human)

Use

Gateway entry vector

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe NGF-EGFR-FRB-GluGlu neo

Plasmid

#192293

Purpose

Retroviral expression vector for NGF-EGFR-FRB-GluGlu construct

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

NGF-EGFR-FRB-GluGlu (EGFR Human)

Use

Retroviral

Tags

GluGlu

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe NGF-Erbb2-2xFKBP-HA hygro

Plasmid

#192291

Purpose

Retroviral expression vector for NGF-Erbb2-2xFKBP-HA construct

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

NGF-Erbb2-2xFKBP-HA (Erbb2 Rat)

Use

Retroviral

Tags

HA

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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p426-Cup1p-FusionRed

Plasmid

#188392

Purpose

Cu2+-dependent expression of red fluorescent protein in yeast cells

Depositor

Michihiko Kataoka

Article

Utsumi et al PLoS One. 2023 Apr 13;18(4):e0283002. do

Insert

FusionRed

Expression

Yeast

Promoter

CUP1 promoter

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc_S1632X

Plasmid

#198122

Purpose

Express hCOL4A5_S1632X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Tags

Nluc

Expression

Mammalian

Mutation

S1632X

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc_Q1016X

Plasmid

#198119

Purpose

Express hCOL4A5_Q1016X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Tags

Nluc

Expression

Mammalian

Mutation

Q1016X

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge