We narrowed to 20,483 results for: Cre

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pCSIIbsr-6013x

Plasmid

#108654

Purpose

An HRas biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

Raichu-EV

Use

Lentiviral

Tags

XFP

Expression

Mammalian

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a_SPIB_P2A_Hygro_Barcode

Plasmid

#120483

Purpose

Barcoded lentiviral vector to express SPIB in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SPIB (SPIB Human)

Use

Lentiviral

Promoter

EF1a

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB509

Plasmid

#74851

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 6xHis (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3_5UTR_Myc_FLuc

Plasmid

#229502

Purpose

Myc 5' untranslated region firefly luciferase reporter.

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

Myc 5'UTR

Expression

Mammalian

Promoter

SV40

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Consensus

Plasmid

#176678

Purpose

Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB420

Plasmid

#74814

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-BFP

Plasmid

#106282

Purpose

A version of the pHAGE vector with BFP and dsRed expressed separated by an IRES. Note that dsRed is not expressed.

Depositor

Jay Shendure

Article

Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

Insert

CMV-BFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-Decoy

Plasmid

#80324

Purpose

Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

Decoy sgRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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SAE1

Plasmid

#175241

Purpose

Bacterial expression and purification, combined with SAE2 to form the E1 enzyme heterodimer

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

SAE1 (SAE1 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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SAE2-mGarnet

Plasmid

#175240

Purpose

Bacterial expression and purification, combined with SAE1 to form the E1 enzyme heterodimer

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBA2 (UBA2 Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Aalb_743

Plasmid

#176662

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAG306-GPD-VMA1 chr I

Plasmid

#41896

Purpose

Yeast expression of VMA1.

Depositor

Dan Gottschling

Article

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Insert

VMA1 (VMA1 Budding Yeast)

Expression

Yeast

Mutation

Contains silent mutation at 342A-G and L87S mutat…

Promoter

GPD

Available Since

April 17, 2013

Availability

Academic Institutions and Nonprofits only

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Lenti-sgArid1a-sg3

Plasmid

#192169

Purpose

Lenti-sgArid1a-sg3

Depositor

Sidi Chen

Article

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

Lentiviral

Mutation

NA

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6020nes

Plasmid

#108656

Purpose

A JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-JNK-EV

Tags

XFP

Expression

Mammalian

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 Y3F uGFP

Plasmid

#14558

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F (Y142, Y160, Y174) in DH domain

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-LMO11 (GeNL_SS-ChRmine)

Plasmid

#213034

Purpose

fusion protein of mNeonGreen, SSLuc, and ChRmine for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Slaviero et al Neurophotonics. 2024 Apr;11(2):024208. d

Insert

mNeonGreen, ISN-IE, ChRmine

Use

AAV

Tags

Gaussia luciferase signal sequence

Promoter

hSyn

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV Ef1a DIO GeNL_SS hGtACR1

Plasmid

#233511

Purpose

Luciferase opsin fusion protein (luminopsin) for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Ikefuama et al iScience. 2025 Feb 13;28(3):112022. doi:

Inserts

mNeonGreen
ISN-IE
hGtACR1

Use

AAV

Tags

Gaussia luciferase signal sequence

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-LOx-IRES-EGFP

Plasmid

#166877

Purpose

Express the bacterial lactate oxidase (LOx) enzyme in mammalian cells

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

Insert

Lactate oxidase

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-PAK2 wt

Plasmid

#31671

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 (PAK2 Human)

Tags

GST

Expression

Bacterial

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pVk7-33G1-Flag

Plasmid

#58950

Purpose

backbone for mouse recombinant antibody with added C-terminal FLAG tag

Depositor

Gavin Wright

Article

Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

Type

Empty backbone

Tags

FLAG tag (DYKDDDDK)

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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