We narrowed to 14,442 results for: NTS

1. pBSFI-myr-akt-T308D/S473D

   Plasmid

   #49192

   Purpose

   cDNA of Akt1 with myristyolation signal and point mutation from T to D at position 308 and S to D at position 473 in cloning vector

   Depositor

   Peter Vogt

   Article

   Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Adaptor plasmid

   Tags

   HA tag and myristolation signal

   Mutation

   T308D; S473D

   Promoter

   T7

   Available Since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. tetO-GATA4

   Plasmid

   #46030

   Depositor

   John Gearhart

   Article

   Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

   Insert

   GATA4 (GATA4 Human)
   

   Use

   Lentiviral

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   R43Q, G50S and N197S

   Promoter

   tetO

   Available Since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-myc-HLA-A24

   Plasmid

   #135507

   Purpose

   Mammalian expression of myc-tagged HLA-A*024:02

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*24:02 (HLA-A Human)
   

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. PET-Sniper SpCas9-NLS-6xHis

   Plasmid

   #207374

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Sniper SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F539S, M763I, K890N

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGreenFire_blastocidin

   Plasmid

   #174103

   Purpose

   pGreenFire MPRA plasmid for cloning

   Depositor

   Paul Khavari

   Article

   Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. HiBiT-FZD8

   Plasmid

   #195846

   Purpose

   Expresses human FZD8 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD8 (FZD8 Human)
   

   Tags

   HiBiT

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 His-NLRP1 codon-optimized

   Plasmid

   #166833

   Purpose

   Mammalian codon-optimized NLRP1 with N-terminal and C-terminal HIS tags

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Tags

   HIS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. 5_T7-GAP43-mScarlet3

   Plasmid

   #225932

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the GAP43 membrane localisation signal

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   GAP43-mScarlet3
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti_PGK-Cas9-P2A-HygR

   Plasmid

   #164135

   Purpose

   Lentiviral construct for the expression of SpCas9 driven by PGK promoter in mammalian cells

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Insert

   SpCas9 (cas9 Synthetic)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ-ABE-Cas9n-Act3.0

    Plasmid

    #178956

    Purpose

    It consists of a Cas9 nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Insert

    ABE8e-zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-eNme2-C-BE4max

    Plasmid

    #183679

    Purpose

    Expresses eNme2-C-BE4 in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    rAPOBEC1-neNme2-C-2xUGI
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. SPOP-V5 (BTB+BACK)

    Plasmid

    #169723

    Purpose

    Mammalian Expression of mouse SPOP-BTB+BACK (L186D, L190D, L193D, I217K, Y353E)

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    SPOP (Spop Mouse)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    L186D, L190D, L193D, I217K, Y353E

    Promoter

    CMV

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-MPRA-MluI-SpeI-EcoRI

    Plasmid

    #190196

    Purpose

    Empty vector for inserting MPRA libraries into AAV

    Depositor

    Hyejung Won

    Article

    McAfee et al Cell Genom. 2023 Sep 15;3(10):100404. do

    Type

    Empty backbone

    Use

    AAV; Massively parallel reporter assay (mpra)

    Expression

    Mammalian

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCK341

    Plasmid

    #192640

    Purpose

    Expresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Inserts

    Sp.pCas9-dSpRY
    BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    SoxS has R93A and S101A mutations and dSpRY has s…

    Promoter

    BBa_J23107 and Sp.pCas9

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV N-term HA-FLAG puro BRD7

    Plasmid

    #41934

    Depositor

    Stephen Elledge

    Article

    Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    BRD7 (BRD7 Human)
    

    Use

    Retroviral

    Tags

    HA-Flag

    Expression

    Mammalian

    Available Since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pIRES-hrGFP II-TET1

    Plasmid

    #83568

    Purpose

    Expresses TET1 in mammalian cells

    Depositor

    Jean-Pierre Issa

    Article

    Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

    Insert

    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    

    Tags

    3X FLAG tag and hr GFP II

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. LV_EF1a_PTEN(C124S)-P2A-Hygro_Barcode

    Plasmid

    #170248

    Purpose

    Barcoded lentiviral vector to express PTEN (C124S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Mutation

    C124S

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Rat Alpha2 delta-1 HA pMT2

    Plasmid

    #58729

    Purpose

    expression of HA tagged rat Alpha2 delta-1 in mamalian cells

    Depositor

    Annette Dolphin

    Article

    Kadurin et al J Biol Chem. 2012 Sep 28;287(40):33554-6

    Insert

    alpha2 delta-1 (Cacna2d1 Rat)
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1_Zeo_ARRB2-1-383-CTEV-2xHA

    Plasmid

    #194382

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    ARRB2-CTEV-2xHA (ARRB2 Human, Synthetic)
    

    Tags

    2xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S

    Plasmid

    #166794

    Purpose

    Mammalian cell expression vector for Flag-tagged DPP9S

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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