We narrowed to 15,953 results for: grna

1. pBWB424

   Plasmid

   #140638

   Purpose

   RSF1010-Cas9-vanAB gRNA. Vector with constitutive Cas9 and gRNA targeting vanAB locus.

   Depositor

   Keith Tyo

   Article

   Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

   Insert

   Cas9
   

   Expression

   Bacterial

   Available Since

   May 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330A-FBL/PITCh

   Plasmid

   #63671

   Purpose

   Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pBWB419

   Plasmid

   #140637

   Purpose

   RSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.

   Depositor

   Keith Tyo

   Article

   Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

   Insert

   Cas9
   

   Expression

   Bacterial

   Available Since

   May 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cas9-GFP_sg_mAMPKa1

   Plasmid

   #79004

   Purpose

   Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Prkaa1 (Prkaa1 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pXSE901BG

   Plasmid

   #91714

   Purpose

   CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance

   Depositor

   Qi-Jun Chen

   Article

   Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHSE901

   Plasmid

   #91708

   Purpose

   CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, HygR

   Depositor

   Qi-Jun Chen

   Article

   Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSAG1::CAS9-U6::sgUPRT

   Plasmid

   #54467

   Purpose

   Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.

   Depositor

   David Sibley

   Article

   Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.

   Insert

   SAG1 5' UTR
   

   Use

   CRISPR; Toxoplasma expression

   Available Since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPEG

   Plasmid

   #162466

   Purpose

   Entry vector for gRNA insertion in plant prime editing expression vector

   Depositor

   Adam Bogdanove

   Article

   Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

   Type

   Empty backbone

   Use

   Entry vector for grna

   Promoter

   CmYLCV

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEn-Chimera

   Plasmid

   #61432

   Purpose

   Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone

   Depositor

   Holger Puchta

   Article

   Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

   Insert

   AtU6-26:sgRNA
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   AtU6-26

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEP163

    Plasmid

    #137881

    Purpose

    PiggyBac donor vector for expression of mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    mRFP-T2A-BlasticidinR
    

    Expression

    Mammalian

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ141B2.0

    Plasmid

    #99897

    Purpose

    Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU3

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRGE32

    Plasmid

    #63159

    Purpose

    Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast

    Depositor

    Yinong Yang

    Article

    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    Rice snoRNA U3 promoter for gRNA/PTG expression a…

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRN227

    Plasmid

    #127222

    Purpose

    BeYDV replicon with 35S::Cas9, WUS2 and AtSTM, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Cas9, WUS2, AtSTM, sgRNA
    

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ141D2.0

    Plasmid

    #99906

    Purpose

    Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    OsU3

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXPR_501

    Plasmid

    #96922

    Purpose

    for CRISPRa, lentiviral expression of gRNA scaffold using activator VP64

    Depositor

    David Root, John Doench

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCP-tRNA

    Plasmid

    #133812

    Purpose

    CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis

    Depositor

    Geraldine Butler

    Article

    Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

    Insert

    cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
    

    Use

    CRISPR

    Available Since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYN2_1

    Plasmid

    #184757

    Purpose

    Plasmid for genome editing by CRISPR/Cas9

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    Cas9
    sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
    

    Use

    Synthetic Biology

    Tags

    2xNLS

    Expression

    Bacterial and Yeast

    Promoter

    PGK1 and tRNA-Phe-HDV Ribozyme

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMM114

    Plasmid

    #127211

    Purpose

    BeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    WUS2, IPT, sgRNA, Luc
    

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBSE901

    Plasmid

    #91709

    Purpose

    CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistance

    Depositor

    Qi-Jun Chen

    Article

    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMM113

    Plasmid

    #127210

    Purpose

    BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    WUS2, AtSTM, sgRNA, Luc
    

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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