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We narrowed to 14,665 results for: sta

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Showing: 8121 - 8140 of 14665 results

  1. pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2

    Plasmid
    #105801
    Purpose
    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: FLAG-3C; ORF2: mClover3-TEV
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105801-plasmid-map-sequence-id-203395

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  4. pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening))

    Plasmid
    #239448
    Purpose
    Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing.
    Depositor
    Alexis Komor
    Inserts
    S.p. gRNA backbone
    S.a. gRNA backbone
    PuroR-T2A-BFP(C>T_screening)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    BFP: T65S, S72S, V145F, K206K, H231L
    Promoter
    EF1alpha, U6, and mU6
    Available since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239448-plasmid-map-sequence-id-476590

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  9. pGBW-m4164344

    Plasmid
    #153554
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    accatg Kozak-START linker
    Expression
    Mammalian
    Mutation
    Gordon et al recoded
    Available since
    June 16, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153554-plasmid-map-sequence-id-300140

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  10. pEG610

    Plasmid
    #40195
    Depositor
    Geraldine Seydoux
    Publication
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (aa1-355) (RNAi resistant) (mex-5 Nematode)
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    contains aa 1-355; exon 2 recoded to be RNAi-resi…
    Promoter
    4.4 kb mex-5 promoter fragment
    Available since
    Sept. 20, 2012
    Availability
    Academic Institutions and Nonprofits only

  11. pEG585

    Plasmid
    #40192
    Depositor
    Geraldine Seydoux
    Publication
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (S404A) (RNAi resistant) (mex-5 Nematode)
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    S404A; exon 2 recoded to be RNAi-resistant
    Promoter
    4.4 kb mex-5 promoter fragment
    Available since
    Sept. 13, 2012
    Availability
    Academic Institutions and Nonprofits only

  12. pTT3-ecdCD19-ST3-His_withSUMO

    Plasmid
    #223219
    Purpose
    Expression of the extracellular domain of human CD19 fused to Spytag003 + Histag in HEK293(or similar) with SUMO for better protein expression
    Depositor
    Omer Dushek
    Publication
    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.
    Insert
    extracellular domain of B-lymphocyte antigen CD19 (CD19 Human)
    Tags
    Histag, SUMO, and Spytag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    223219-plasmid-map-sequence-id-443124

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  14. pET151-D-TOPO-PARP14-SΔK

    Plasmid
    #216658
    Purpose
    Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.
    Depositor
    Herwig Schüler
    Publication
    Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.
    Insert
    PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Synthetic, Human)
    Tags
    6xHis-V5-TEV
    Expression
    Bacterial
    Mutation
    Deletion starting from Q1390 to D1448 and substit…
    Promoter
    T7
    Available since
    Aug. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    216658-plasmid-map-sequence-id-429340

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  15. pDIV313

    Plasmid
    #204956
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. niger
    Depositor
    Uffe Mortensen
    Publication
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    hph (hygromycin resistance marker)
    sgRNA (CAGCAATGCTTCCATGCAATT) targeting albA locus in A. niger flanked by a tRNA-Gly repeat
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus fumigatus U3 promoter, Aspergillus ni…
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    204956-plasmid-map-sequence-id-415598

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  16. piggyBac_rtTA (4th_Gen)_Gateway_IRES_NGN2-2A-PURO_Synapsin-BirA. (pEHA1618)

    Plasmid
    #209084
    Purpose
    Ngn2 mediated cortical neuron induction, gateway destination, Synapsin promoter BirA ligase expression
    Depositor
    Vikram Khurana
    Publication
    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    NGN2 (Neurog2 Mouse)
    BirA
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    hSyn
    Available since
    Nov. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    209084-plasmid-map-sequence-id-412874

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  19. pAcBac1-Mb-Fluoro-Phe D6

    Plasmid
    #197577
    Purpose
    Expression of Methanosarcina barkeri (Mb) Fluoro-Phe D6 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Publication
    Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10
    Inserts
    Mb Pyl Fluoro-Phe B5 synthetase
    Pyl-tRNA (4 copies)
    Tags
    Nuclear export sequence + FLAG
    Expression
    Mammalian
    Mutation
    N311A, C313M, V366G, W382T
    Promoter
    CMV and U6/H1
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197577-plasmid-map-sequence-id-388240

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  20. pDGB1alpha11_SulR

    Plasmid
    #186424
    Purpose
    transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter; suitable for quintuple assembly
    Depositor
    Lukas Fischer
    Publication
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Insert
    SulR
    Use
    Synthetic Biology; Binary vector for escherichia …
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186424-plasmid-map-sequence-id-369067

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Showing: 8121 - 8140 of 14665 results