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pCLIP9

Plasmid

#125141

Purpose

General purpose cloning vector

Depositor

Chris Boyd, David Sherratt

Article

Boyd et al Gene. 1995 Feb 3;153(1):57-62.

Type

Empty backbone

Expression

Bacterial

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pXf20pemIK-GW

Plasmid

#126576

Purpose

Vector for gene expression in Xylella fastidiosa, includes pemI/pemK toxin-antitoxin system for plasmid stability, Gateway cloning compatible

Depositor

Lindsey Burbank, Drake Stenger

Article

Burbank et al Phytopathology. 2016 Aug;106(8):928-36.

Type

Empty backbone

Expression

Bacterial

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCLIP19

Plasmid

#125143

Purpose

General purpose cloning vector

Depositor

Chris Boyd, David Sherratt

Article

Boyd et al Gene. 1995 Feb 3;153(1):57-62.

Type

Empty backbone

Expression

Bacterial

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pSR357.27

Plasmid

#125110

Purpose

Expression of narL(1-120aa)-ydfI(115-213aa) chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-ydfI(DBD)120
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.28

Plasmid

#125087

Purpose

Expression of ompR(1-138aa)-ccaR(128-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)138
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.26

Plasmid

#125086

Purpose

Expression of ompR(1-113aa)-ccaR(110-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

OmpR(REC)-ccaR(DBD)113
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pKD283.2

Plasmid

#125101

Purpose

Expression of SO_4487(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4487(REC)-psdR(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pKD275

Plasmid

#125097

Purpose

Expression of SO_0622(1-129aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_0622(REC)-psdR(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.30

Plasmid

#125089

Purpose

Expression of ompR(1-142aa)-ccaR(132-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)142
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Mutation

L119M- see depositor comments below

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.18

Plasmid

#125084

Purpose

Expression of ompR(1-150aa)-ccaR(140-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)150
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.14

Plasmid

#125081

Purpose

Expression of ompR(1-122aa)-ccaR(119-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)122
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.13

Plasmid

#125080

Purpose

Expression of ompR(1-118aa)-ccaR(115-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)118
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSR300.3

Plasmid

#125079

Purpose

Expression of uhpA under PLtetO-1, output Promoter PuhpT99

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

uhpA (uhpA )
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AX

Plasmid

#122726

Purpose

pSKAP plasmid containing the unique DNA barcode "AX" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AX

Expression

Bacterial

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AW

Plasmid

#122725

Purpose

pSKAP plasmid containing the unique DNA barcode "AW" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AW

Expression

Bacterial

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AV

Plasmid

#122724

Purpose

pSKAP plasmid containing the unique DNA barcode "AV" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AV

Expression

Bacterial

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AT

Plasmid

#122722

Purpose

pSKAP plasmid containing the unique DNA barcode "AT" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AT

Expression

Bacterial

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AS

Plasmid

#122721

Purpose

pSKAP plasmid containing the unique DNA barcode "AS" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AS

Expression

Bacterial

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AQ

Plasmid

#122719

Purpose

pSKAP plasmid containing the unique DNA barcode "AQ" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AQ

Expression

Bacterial

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AP

Plasmid

#122718

Purpose

pSKAP plasmid containing the unique DNA barcode "AP" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AP

Expression

Bacterial

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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