We narrowed to 15,733 results for: LEA

1. pCMV-T7-TadCBEd-SpCas9(MKRCKS)-P2A-EGFP (RAS3445)

   Plasmid

   #223130

   Purpose

   pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA-CDd-SpCas9(MKRCKS)-2xUGI-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

   Expression

   Mammalian

   Mutation

   TadA-CDd mutations; nSpCas9(D10A/MKRCKS)=D1135M, …

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-TadCBEd-SpCas9(ARGIMR)-P2A-EGFP (BKS1036)

   Plasmid

   #223132

   Purpose

   pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with ARGIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA-CDd-SpCas9(ARGIMR)-2xUGI-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

   Expression

   Mammalian

   Mutation

   TadA-CDd mutations; nSpCas9(D10A/ARGIMR)=D1135A, …

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-TadCBEd-SpCas9(MRKCRS)-P2A-EGFP (AHK545)

   Plasmid

   #223133

   Purpose

   pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA-CDd-SpCas9(MRKCRS)-2xUGI-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

   Expression

   Mammalian

   Mutation

   TadA-CDd mutations; nSpCas9(D10A/MRKCRS)=D1135M, …

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. t1-405(151-154AAAA)

   Plasmid

   #166131

   Purpose

   Expression of human talin-1 head (aa1-405) in E. coli. Four substitutions in the F1-loop (aa151-154 all mutated to Al). N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site.

   Depositor

   Vesa Hytönen

   Article

   Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

   Insert

   T1head1-405(151-154AAAA) (TLN1 Human)
   

   Tags

   His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

   Expression

   Bacterial

   Mutation

   aa1-405 only. Contains 4 amino acid substitutions…

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL4-eIF3B MBE3 mutant

   Plasmid

   #133310

   Purpose

   Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 3

   Depositor

   Josep Domingo-Domenech

   Article

   Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

   Insert

   eIF3B promoter MBE3 mutant (EIF3B Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   ccacgtgacc changed to cAaAAAAacc

   Promoter

   eIF3B

   Available Since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4-eIF3B MBE2 mutant

   Plasmid

   #133309

   Purpose

   Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 2

   Depositor

   Josep Domingo-Domenech

   Article

   Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

   Insert

   eIF3B promoter MBE2 mutant (EIF3B Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   gccacatgcacc changed to gcAaAaAAcacc

   Promoter

   eIF3B

   Available Since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. px330 Gatad2a sgRNA (for flox targeting)

   Plasmid

   #110815

   Purpose

   Used with pNTK Gatad2a flox allele targeting construct in mouse cells

   Depositor

   Jacob Hanna

   Article

   Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

   Insert

   mGataD2a (Gatad2a Mouse)
   

   Expression

   Mammalian

   Available Since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL4.23-IL2RA CaRE2 (minus strand)

   Plasmid

   #91847

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0624)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE2 (minus strand) (IL2RA Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   rs6602425 A>C, G>A mutation at chr10:611706…

   Available Since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4.23-IL2RA CaRE1 (minus strand)

   Plasmid

   #91838

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0615)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE1 (minus strand) (IL2RA Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Perfect match to reference sequence

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLCAR-CD19-28z

    Plasmid

    #135991

    Purpose

    Modular CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Scott McComb

    Article

    Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

    Insert

    FMC63-28-3z-P2A-EGFP (CD28, CD247 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a-short

    Available Since

    June 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-EF1A-CD19.28H.28TM.28.3z

    Plasmid

    #200678

    Purpose

    Modular CD28hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD28HD-CD28TM-CD28-CD3z-P2A-EGFP (CD28, CD247 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Promoter

    EF1a-short

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-EF1A-CD19.28H.28TM.BB.3z

    Plasmid

    #200679

    Purpose

    Modular CD28hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD28HD-CD28TM-41BB-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Promoter

    EF1a-short

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLCAR-CD19-CD3z

    Plasmid

    #135993

    Purpose

    Modular CD3z alone CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Scott McComb

    Article

    Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

    Insert

    FMC63-3z-P2A-EGFP (CD247, CD28 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a-short

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE2-TetOminiCMV-OSKM

    Plasmid

    #136541

    Purpose

    Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4, cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Sox2, Pou5f1, Myc, Klf4 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUltra-MDH1-ME1

    Plasmid

    #184465

    Purpose

    Lentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Inserts

    Malate dehydrogenase 1 (MDH1 Human)
    Malic Enzyme 1 (ME1 Human)
    

    Use

    Lentiviral

    Tags

    fused to T2A

    Expression

    Mammalian

    Mutation

    deletion of stop codon

    Promoter

    UbC

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLPC-puromycin-binary-MDH1-3xFLAG-ME1- HA

    Plasmid

    #184549

    Purpose

    Retroviral vector to co-express human MDH1 with 3xFLAG tag and human ME1 with HA tag

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Inserts

    Malate dehydogenase 1 (MDH1 Human)
    Malic Enzyme 1 (ME1 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG and HA tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEMS1353

    Plasmid

    #29155

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple137 (NR2E1 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Available Since

    Nov. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEMS1352

    Plasmid

    #29154

    Purpose

    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple136 (NR2E1 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Available Since

    Nov. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEMS1351

    Plasmid

    #29153

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple135 (NR2E1 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Available Since

    Nov. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBIG1a-PAF1CdeltaWDR61

    Plasmid

    #231745

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CTR9 (CTR9 Human)
    CDC73 (CDC73 Human)
    PAF1 (PAF1 Human)
    LEO1 (LEO1 Human)
    

    Tags

    3C-Strep(II) and Flag-3C

    Expression

    Insect

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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